Mining of saline-alkali resistant elements with multi-group learning
When six kinds of saline-alkali-tolerant bacteria are faced with saline-alkali stress environment, they show consistent changes in gene expression, protein translation and metabolic regulation. This common gene is the universal stress-resistant element we are looking for. After analyzing the genes and expressed proteins of their difference table, we obtained the most likely universal element of salt resistance. See Table 5.1
< img src="https://static.igem.wiki/teams/4835/wiki/shiyan4/tb5-1.png" style="width: 30%;">
Table 5.1 the most likely universal components for saline-alkali resistance
qPCR test
We then performed qPCR tests and the up- and down-regulation results were consistent with the transcriptomics results.
< img src="https://static.igem.wiki/teams/4835/wiki/shiyan5/fig5-3.png" style="width: 30%;">