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Polycobead Cell-free Production
Legal analysis Proposal T.O.M.A.T.O.

Overview

Plant fungal diseases are one of the main culprits affecting the quality and yield of crops. Their existence has greatly impacted food health and agricultural development, and may even further increase the degree of hunger. These diseases are caused by fungi, which secrete a variety of pathogenic factors to attack the host when they infect crops, and can spread widely before effective measures are taken.


Botrytis cinerea is a kind of worldwide pathogen of gray mold. It can infect the roots, leaves, flowers and fruits of plants, causing diseases on more than 500 plant species, including more than 200 crop species. B. cinerea is a plant pathogen suitable for growing in low temperature and high humidity. In agricultural production, the temperature in the greenhouse is usually maintained at 15-20°C, with high relative humidity, which is easy to cause a large range of gray mold. B. cinereaa is one of the most damaging post-harvest pathogens in many agricultural products. In the process of storage and transportation after harvest, B. cinerea will accelerate the deterioration of crops, resulting in serious logistics losses.


Products and application

PolycoBead

POLYCOBEAD is an efficient composite green biopesticide that employs RNAi technology for gene silencing of B. cinerea, and uses engineered bacteria to induce immune responses in plants. It is designed to treat and prevent various forms of gray mold disease in over 200 crops, including tomatoes, strawberries, grapes, and more. PolycoBead aims to provide your plants with optimal growth and protection.


PolycoBead

Step 1: Preparation


  • -Ensure that the plants are growing under suitable conditions and use during the high-risk period for plant fungal diseases. It is recommended to use during the flowering and fruiting stages of the crops.

  • -Calculate the size of the farmland and crop planting density using the following formula: Mix 2 PolycoBeads with 10 liters of water to treat approximately 100 square meters of tomato fields with a density of approximately 100-120 plants per square meter, each plant having 50-60 fruits. The point concentration of CPP-shRNAs is approximately 10 μg.

Step 2: Application


  • - Mix 2 PolycoBeads with 10 liters of water using a clean stick until the PVA outer membrane ruptures, and the contents are released and thoroughly mixed.

  • - Spray the liquid evenly in the field using a sprayer or a drone while evenly distributing the calcium alginate beads near the plant roots.

Step 3: Maintenance


  • - Monitor the plant's growth over time.

  • - Based on the plant's growth, apply PolycoBeads every 8-10 days.



Cell-free Production


Automatic Hardware System

An Automated Equipment for Large-Scale Production of shRNA Molecules through a Cell-Free Method: We have designed and built an automated hardware system suitable for the large-scale synthesis of shRNA molecules. By programming a microcontroller, we have established an automated production process for shRNA molecules, allowing for their convenient manufacturing without the need for manual intervention. This system can be operated by non-scientists and can be applied in non-laboratory environments. It aims to enhance the convenience of shRNA production while reducing associated costs and opens up the possibility of further scaling up production.


The equipment primarily comprises three peristaltic pumps, a reaction container, and a temperature control module. Our equipment has demonstrated the successful synthesis of shRNA molecules on a significantly larger scale when compared to traditional PCR instruments. This automated equipment presents a cost-effective, user-friendly, and scalable solution for shRNA molecule synthesis, thereby enhancing the accessibility and efficiency of RNA interference technology across diverse applications.




Promotion

Legal analysis

New composite RNAi biopesticide are relatively advanced and have not yet formed a considerable scale in today's markets. Therefore we consider that there may not be appropriate laws for this emerging products. Before a large number of new products flood into the market, we read lots of relevant laws in advance to investigate the applicability of existing laws to newly emerging products.


We have extensively read the relevant laws and regulations that our products need to comply with, especially the classification standards and policy trends involving cutting-edge technologies such as RNAi technology and plant immune agents. We summarized them and wrote an overall review of relevant laws, which may serve as comprehensive reference and reliable consultant for this industry.



The analysis of laws and regulations will help to ensure that our products can be commercialized more smoothly, and lay a solid legal and regulatory foundation for the future development of our Polycobead.


The Proposal of Chinese RNA Pesticide Industry Standard

Through a large number of laws and regulations analysis, we found that the existing laws for new products still have some defects and imperfection. For cutting-edge technologies and emerging products, there is always a certain lag in the current law. Before new products flood into the market, there is an urgent need to bridge the gap by establishing applicable regulations and standards to ensure safety and sustainability in this field.


Out of a sense of safety and social responsibility, we have drawn up The Proposal of Chinese RNA Pesticide Industry Standard based on our own experience, expert consultation and the results of field investigations, and have standardized and identified various safety indicators, functions and responsibilities.



We also presented the draft to Director Li of Shenzhen Agricultural Promotion Center. We discussed the content of the draft, absorbed the teacher's suggestions and put forward our opinions. This attempt has been praised and affirmed by the government, and it is also of great significance for the future pesticide industry.


T.O.M.A.T.O.

The laws and regulations together with the industry standards create an orderly market environment for the new composite RNAi biopesticides.



We analyze the needs and market background of each units, and put forward a strategic plan FROM UNIT TO UNION. We unit each units to form a union called the T.O.M.A.T.O Alliance to promote the transformation and upgrading of the pesticide industry.



This alliance will consists of two parts: technical alliance and thought alliance. We are paving the way for the future of RNAi biopesticide from two perspectives: technological transformation and social recognition.



We found that the emergence of new products will inevitably pose a threat to the traditional chemical pesticide industry. In order to avoid conflicts between new and old products, we put forward the concept of Technology-Oriented Modernization of Agriculture-Targeted Organizations (Technology lliance). We call on advanced biotechnology enterprises to join the biopesticide research, but also guide traditional enterprises to transform and upgrade.

By combining the technical support of biotech companies with the market foundation of traditional enterprises, we believe that we can promote the steady progress of the pesticide industry and accelerate its transformation and upgrading.


Besides products markets, we form the concept of Thought-Oriented Modernization of Agriculture-Targeted Organizations (Thought Alliance). We hope to gather the power of different people to arouse people's awareness of green agriculture and ecological protection, as well as removing obstacles for the landing and promotion of our products.

We hope to rally the power of knowledgeable individuals from various sectors to promote awareness of using biopesticides. Besides focusing on product and market aspects, we aim to raise people's attention towards green agriculture and ecological protection, forging ahead towards a blueprint for zero pollution.


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