"What we are doing, why we are doing it, how the world might affect our work, and how our work might affect the world."
              
                Our core tenet of human practice is to bridge the gap between
                our projects and the real world and by continuously interacting
                with the world. During the process, we improve our products
                through close contact with stakeholders by gaining a deep
                understanding of their needs and opinions.
              
              
              
              Our team put forward an innovative approach to execute our
              integrated human practice --
              
                "5I cycle" (Inspiration, Ideation, Information collecting,
                Improvement and interaction, Implementation and imagination).
              
              This cycle helps ensure that we engage respectfullly and
              responsibility with stakeholders.
              
              
            
            Inspiration
The experience of HIGREEN
"Tomato Crisis"
                
                  Higreen, a major agricultural wholesale market in Shenzhen,
                  supplies over 90% of the city's fruits and vegetables. One day
                  when we were buying tomatoes in HIGREEN as usual, we
                  encountered Mr. Cheng uploading crates of tomatoes covered
                  with strange white spots. We inquired about the situation, and
                  Mr. Cheng, told us that the tomatoes was suffering gray mold
                  disease. As a result, they even affected other tomatoes that
                  were not infected during the transportation process, and led
                  to significant financial loss.
                  
                  
                  
                    Mr. Cheng expressed deep concern and frustration as he
                    talked about the significant threat posed by gray mold
                    disease to his tomato planting base -- Dacheng Xin Base. He
                    highlighted that this disease posed the most severe
                    challenge. Regrettably, it had even led to devastating
                    consequences, resulting in yield reductions of up to 50%.
                    Moreover, he lamented the lack of an effective solution to
                    combat this persistent and damaging disease.
                  
                  
                
              
              After listening to Mr. Cheng's concerns, our team were eager to
              assist him in solving this challenging issue. We conducted further
              research on gray mold disease in tomatoes :
              
              
              Significant Threat of Gray Mold Disease :
              Based on 2020 FAO data, global tomato production reached
              approximately 189 million metric tons, with China contributing a
              significant 68 million metric tons. However, the devastating
              impact of tomato gray mold disease led to production losses
              ranging from 20% to 60%.Based on statistics from 2016, tomato gray
              mold disease annually caused more than 30 million yuan losses in
              China worth
              
              
            
            
              Current Control Measures: physical and chemical control.
              Physical control methods include proper crop rotation and
              cultivation management practices. Chemical control is the most
              widely used method, involving the use of pesticides such as
              dimethomorph and pyrimethanil to manage the spread of the disease.
              
              
              
              Shortcomings and Drawbacks:
              
            
- 1. Overusing chemical pesticides tend to make the fungus resistant to drug , reducing drug effectiveness.
 - 2. Pesticide residues and emissions threaten the environment, potentially harming soil and water quality.
 - 3. The destroyed environment cost extra money to undo the effects.
 
              
              
            Ideation and project forming
Discussing with the PI
"RNAi, Efficient and Eco-friendly"
                    After learning about the harmful result caused by gray mold
                    disease, we wanted to
                    find an efficient and environmentally friendly way
                    to cure the disease. So, we consulted our PI, Professor Mo
                    Beixin, who made great achievements in botanical research.
                    Upon hearing our needs and ideas, Professor Mo shared us
                    with valuable insights, suggesting that RNA interference
                    (RNAi) technology could be a potential solution. Also it has
                    many advantages:
                    
                    
                    1.Precision:
                    RNAi technology precisely targets and silences harmful
                    pathogen genes for highly focused control.
                    
                    2.Efficacy:
                    It effectively reduces disease severity by inhibiting
                    crucial pathogen genes.
                    
                    3.Non-Genetic:
                    RNAi avoids genetic alterations, relying on natural
                    mechanisms for temporary gene silencing.
                    
                    4.Safety:
                    It is safe, as it doesn't introduce foreign genes or alter
                    host genomes, minimizing risks.
                    
                    5.Environmentally Friendly:
                    RNAi naturally degrades, leaving no lasting environmental
                    residues.
                    
                  
                  
                  Communicatng with Farmers
"Early Prevention is Crucial"
                  
                  
                    
                    In March, Mr. Cheng invited us to visit the Dacheng Xin
                    base. We went on a field trip, learning from tomato grower
                    Ms Wang that farmers rely heavily on field observation. They
                    predict potential plant diseases by closely monitoring
                    weather, environmental conditions and plant growth. This
                    allows them to take actions to spray pesticide in advance.
                    
                    
                    
                      For producers, the more yielding, the better. They not
                      only hope to effectively treat diseases, but also hope to
                      achieve early prevention. This approach safeguards crop
                      health, reduces disease impact on yield and quality,
                      ensuring better economic returns.
                    
                    
                  
Brainstorming
"Establishing Our Initial Project Design"
                    After in-depth discussions with PI Professor Mo and field
                    trips to the Dacheng Xin Tomato Base, we did broad
                    brainstorm to put forward our initial disease control
                    strategy.
                    
                      Our initial strategy combines prevention and treatment
                      measures to effectively solve the probelm of tomato gray
                      mold disease, ensuring both yield and quality.
                    
                    
                    
                    By integrating preventive actions and timely treatment, we
                    took every stages of gray mold disease into consideration,
                    minimizing the risk for tomatoes to be infected by the gray
                    mold disease. This approach ensures the and economic returns
                    and sustainability of agriculture. We will develop specific
                    plans based on this strategy to tackle the issue of tomato
                    gray mold disease.
                    
                  
                  
                  Expert Interviewing
"Positive Response Towards Initial Project Design"
                  
                  
                    Once we formed our initial disease control strategy, we
                    sought advice form Professor Xiaojie Li, vice president of
                    Shenzhen Graduate school Chinese Academy of Agricultural.
                    Sciences. Professor Li highly commended our project, noting
                    that:
                    'RNA pesticides are the future of the pesticide
                      industry.'
                    He highlighted that RNA pesticides, known for their
                    efficiency and environmental friendliness, are considered
                    the 'third revolution in the pesticide field,' receiving
                    extensive attention and support from national and
                    agricultural stakeholders.
                    
                    
                    Professor Li also fully endorsed the plant immunity
                    component of our project. He emphasized the pivotal
                    principle in plant protection,
                    
                      'prevention is better than cure.', underscoring the
                      importance of proactively prevention to minimize their
                      impact on crops.
                    
                    Professor Li's opinions encouraged us and showed that our
                    initial strategy is of great significance in the
                    agricultural field.
                  
Information collecting
                
                After developing our initial project, we want to assess the
                practical feasibility of our disease control strategy in
                real-world scenarios. Consequently, we conducted interviews with
                stakeholders in the tomato industry to learn about their needs
                and worries regarding the gray mold disease control strategy.
                
                
                
                We hightlight what each stakeholder most concern with towards
                  the usage of pesticides below.
                
              
                  
                    Agricultural Producers
                    
                    
                      Farmers are responsible for tomato cultivation, pest
                      control, and harvesting, and they are the users of
                      pesticides.
                    
                    
                    
                    what they call for:
                    Convenient to Use
                    
                    Efficiency
                    
                    Cheap
                    
                  
                  
                    Tomato Sellers
                    
                    
                      They serve as a bridge within the supply chain,
                      facilitating the transfer of agricultural products from
                      producers to end consumers.
                    
                    
                    
                    what they call for:
                    
                    Cheap
                  
                  
                    Ministry of Agriculture
                    
                    
                      The Ministry of Agriculture are responsible for disease
                      prevention, food safety oversight, and supporting farmers
                      with technology .
                    
                    
                    
                    what they call for:
                    
                    Safe
                    
                    Effective
                    
                    Environmentally Friendly
                    
                    Convenient to Use
                    
                  
                  
                    Consumers
                    
                    Consumers' selective purchasing decisions drive producers
                      and the supply chain towards meeting consumer
                      demands.
                    
                    
                    what they call for:
                    
                    No pesticide residues
                    
                    Environmentally Friendly
                    
                    Safe
                  
                
                 Agricultural Producers
              
              
                  
                  We visited many planting bases and communicated with local
                  farmers to understand the current commonly used pest control
                  methods, as well as their attitudes and needs regarding
                  pesticide use.
                  
                
                    Ms. Wang
a farmer at the Dacheng Xin Tomato Base
                        Ms. Wang shared valuable insights with us. She explained
                        how farmers rely on careful observations of weather,
                        environmental conditions, and especially plant growth to
                        anticipate the potential onset of plant diseases. They
                        take measures like applying pesticides in advance to
                        minimize the chances of plants being affected by
                        diseases.
                        
                        
                        
                          Ms. Wang's proactive approach provided a valuable
                          perspective for our initial project design, leading us
                          to consider the use of plant immunity for early
                          prevention.
                        
                      
Ms. Wang
                        About Gray Mold Disease:
                        Ms. Wang mentioned that gray mold disease is a common
                        issue in the tomato fields she manages. Additionally,
                        diseases like early blight frequently occur, causing her
                        significant distress.
                        
                        
                        Prevention and Control:
                        When facing gray mold disease, Ms. Wang primarily relies
                        on chemical pesticides for control. She informed us that
                        she typically relies on her experience for early
                        prevention. For example, since gray mold disease tends
                        to occur in low-temperature and high-humidity
                        conditions, she proactively sprays pesticides under such
                        weather conditions to prevent it. This proactive
                        prevention approach is crucial because once gray mold
                        disease breaks out, it spreads rapidly. At that stage,
                        pesticide application may not effectively control it,
                        leading to significant crop reduction and economic
                        losses. Ms. Wang's emphasis on early prevention provided
                        us with valuable insights.
                        
                        
                        Concerns About Chemical Pesticides:
                        Ms. Wang also mentioned that most pesticides develop
                        resistance after a few applications, and she cannot use
                        the same pesticide repeatedly. She further explained
                        that some pesticides require mixing, which is
                        challenging for her as she is unsure of the correct
                        blending ratios. Additionally, some pesticides have
                        strong odors that can cause dizziness if she is exposed
                        to them for an extended period.
                      
                    Mr. Liang
                        a farmer in Boluo County
                        
                        
                        He mentioned that the main diseases in his tomato
                        orchard are gray mold and powdery mildew. In disease
                        control, crop rotation and chemical methods are
                        primarily employed. Chemical pesticides need to be used
                        alternately to reduce pests' resistance to them
                        
                        
                        
                          Regarding our treatment plan, Liang emphasized that
                          farmers are most concerned about its effectiveness,
                          followed by cost considerations.
                        
                      
Mr. Liang
                        Regarding gray mold disease:
                        Mr. Liang is a farmer who manages a five-acre tomato
                        field, which operates as a pick-your-own farm during the
                        tomato season. He faces common issues with diseases like
                        gray mold and powdery mildew in his crops. To tackle
                        these problems, he uses methods like crop rotation and
                        high-temperature sterilization.
                        
                        
                        Regarding pesticides:
                        When it comes to pesticides, Mr. Liang and other farmers
                        prioritize safety. They prefer using low-toxicity
                        pesticides and follow advice from trusted suppliers.
                        They also rotate between different types of pesticides
                        to prevent resistance.Mr. Liang shared that after
                        pesticide spraying, there's usually a waiting period
                        before harvesting. This waiting time can lead to
                        economic losses, so he values effective treatments that
                        minimize this downtime.
                        
                        
                        Regarding our treatment solution:
                        Mr. Liang's main concern is its effectiveness in
                        managing diseases. He's willing to invest a bit more if
                        a treatment can provide good results, prioritizing
                        effectiveness over cost. This aligns with our goal of
                        offering practical and efficient solutions to farmers
                        like Mr. Liang, ensuring healthy crops and successful
                        harvests.
                      
                    Hong Chuangjiang
                        Technician at Jiangnong Ecological Agriculture
                          Company
                        
                        
                        Hong's innovative disease management concept emphasizes
                        "treat when there is a disease, prevent when there is
                        none." He focuses on prevention, using microbial agents
                        and organic fertilizers early on, and later, biological
                        agents to adjust soil microbes. Regarding chemical
                        pesticides, he limits the use of the same pesticide to
                        three times due to resistance development.
                        
                        
                        
                          He strongly supports the use of biological pesticides
                          and appreciates our treatment plan, aiming for low or
                          zero pesticide residues in his agricultural products.
                        
                      
Hong Chuangjiang
                        Preventive Measures:
                        Greenhouses, insect-proof nets, and smoke control are
                        physical agricultural measures that help protect crops
                        from pests and diseases. These methods are widely used
                        in agricultural production to ensure the healthy growth
                        of crops.
                        
                        
                        Pest and Disease Management:
                        Mr. Hong Chuangjiang has his own philosophy, which is
                        "treat when diseased, prevent when healthy, and combine
                        Eastern and Western medicine." This philosophy
                        emphasizes the importance of timely treatment for
                        infected plants and proactive prevention of diseases.
                        Preventive measures are considered crucial because they
                        can determine whether crops will be threatened by
                        diseases in the later stages.
                        
                        
                        During the early stages of crop growth, microbial agents
                        and fertilizers are mainly used for prevention, which
                        helps enhance the plants' immune systems. In the later
                        stages of crop growth, microbial agents are employed to
                        adjust the microbial community in the soil, maintaining
                        soil health.
                        
                        
                        Regarding the use of chemical pesticides, a restrictive
                        strategy is typically followed, limiting the number of
                        times a particular pesticide is used. This strategy aims
                        to reduce the development of pesticide resistance and
                        enhance the effectiveness of pesticides.
                        
                        
                        For plants already infected with diseases, isolation
                        methods are employed for management. However, due to the
                        large-scale cultivation, inspection work becomes
                        relatively challenging. Developing an effective
                        identification system could potentially improve the
                        efficiency of disease detection and management.
                        
                        
                        Smart agriculture
                        Smart agriculture is also widely adopted in orchards,
                        utilizing techniques such as greenhouse cultivation,
                        drip irrigation, and digital smart management.
                        Data-driven agriculture management has enabled
                        integrated water and fertilizer management,
                        significantly reducing labor costs. Integrated water and
                        fertilizer systems dissolve fertilizers in irrigation
                        water, enhancing fertilizer efficiency. Despite current
                        challenges related to data accuracy, this field still
                        holds tremendous potential for improving agricultural
                        production efficiency and crop quality.
                      
                    Mr.Cheng
                        Guide and Grower at Dacheng Xin Tomato Planting
                          Base
                        
                        
                        Mr. Cheng discussed tomato cultivation and the key issue
                        of gray mold disease in his fields. They currently rely
                        on chemical pesticides for control, but it's not very
                        effective and leads to substantial losses. He
                        highlighted the need for expert guidance in pesticide
                        use. Farmers often mix pesticides, reducing their
                        effectiveness, and the precise dilution is a challenge
                        for them.
                        
                        
                        
                          Mr. Cheng emphasized his concern about the importance
                          of having user-friendly pesticide application methods
                          that farmers can easily use. Secondly, he is highly
                          concerned about the effectiveness of pesticides to
                          controll tomato gray mold disease.
                        
                      
Mr. Cheng
                        The Millennium Tomato Farm at the Dachengxin Company
                        covers an extensive area of six hundred acres, with two
                        hundred acres personally managed and cultivated by Mr.
                        Cheng. Additionally, Mr. Cheng imparts his extensive
                        cultivation experience to farmers in neighboring
                        villages, providing them with valuable technical
                        guidance for growing cherry tomatoes.
                        
                        
                        Regarding the issue of gray mold disease:
                        Mr. Cheng explained the tomato cultivation process and
                        the primary diseases affecting his tomato fields. Gray
                        mold disease is the predominant ailment in his tomato
                        fields, and they currently employ chemical pesticides
                        for control. However, the effectiveness of this method
                        is somewhat limited, resulting in substantial losses.
                        
                        
                        Concerning pesticide application:
                        Mr. Cheng informed us that pesticide application usually
                        requires the assistance of knowledgeable technicians. He
                        often provides technical guidance to local farmers in
                        nearby villages. He observed that some farmers lack an
                        understanding of pesticide usage rules. Despite the
                        recommended application rates on packaging or in
                        instruction manuals, certain farmers occasionally add
                        pesticides excessively based on their subjective
                        preferences. This leads to increased pesticide residue
                        in crops and, in some cases, contamination of the
                        farmland environment. Furthermore, for pesticide
                        mixtures, farmers lack clear guidance on appropriate
                        proportions for mixing.
                        
                        
                        Regarding Polycobead:
                        We explained our product design and treatment plan to
                        Mr. Cheng. He appreciated the user-friendliness of our
                        product, particularly the convenience of the
                        quantitative encapsulation method using beads. As for
                        our treatment plan, he expressed that his primary
                        concern was its effectiveness. As long as it can provide
                        effective pest control, he would be very willing to use
                        our product.
                      
                
                 Tomato Sellers
              
              
                  
                  We visited many markets and communicated with sellers to learn
                  about consumer's habits and preferences, as well as their
                  views on pesticide residues.
                  
                
                    Cheng Jiabo
                        Sales Representative at Dachengxin Agricultural
                          Company
                        
                        
                        He discussed the challenges in tomato procurement and
                        transportation. Tomatoes often develop gray mold disease
                        before transport, risking the infection of the entire
                        batch during transit. Additionally, agricultural
                        products must undergo pesticide residue testing in the
                        market, exceeding limits can lead to unsellable batches
                        and economic losses.
                        
                        
                        
                          He hopes to effectively control gray mold disease in
                          tomatoes or prevent its spread during transportation.
                          Regarding pesticides, he seeks low residue levels and
                          cost-effective solutions
                        
                      
Cheng Jiabo
                        Regarding gray mold disease:
                        Mr. Cheng informed us that he not only sells tomatoes
                        but also has his tomato farm upstream in the industry.
                        Gray mold disease is a significant issue faced by his
                        tomato farm. It can manifest both before and after the
                        fruits set, making detection and treatment extremely
                        challenging. Currently, there are no effective methods
                        for detection or treatment. The primary approach is to
                        prevent its spread by burning infected plants.
                        
                        
                        Once gray mold disease appears after fruit setting, it
                        spreads rapidly within the crop. While greenhouse
                        cultivation can reduce the incidence of the disease, it
                        comes at a high cost and takes two years to recoup the
                        investment.
                        
                        
                        Although harvested fruits may not initially exhibit
                        symptoms, there is still a risk of fruit rot during
                        transportation due to the presence of gray mold spores.
                        This can lead to significant losses. Furthermore, gray
                        mold disease is highly contagious, exacerbating the
                        potential damage to crops.
                        
                        
                        Regarding biopesticides:
                        We also inquired about Mr. Cheng's views on
                        biopesticides. He mentioned that his primary concern is
                        the cost. While biopesticides are environmentally
                        friendly, their higher prices can lead to an increase in
                        the overall production cost of tomatoes.
                      
                    Mr. Huang
                        The owner of Shenzhen Jinsui Vegetable Wholesale
                          Store
                        
                        
                        Mr. Huang shared that he sells tomatoes from greenhouses
                        and open fields. Greenhouses provide year-round harvests
                        and disease prevention. He also mentioned organic
                        vegetables, grown with organic fertilizers and strict
                        quality standards, which come at higher costs but appeal
                        to environmentally conscious consumers in the growing
                        economy.
                        
                        
                        
                          Regarding our treatment solution,He believes that an
                          eco-friendly pest control method can promote the
                          growth of organic vegetable production, making them
                          more affordable to consumers.
                        
                      
Mr. Huang
                        Regarding tomatoes:
                        Mr. Huang primarily sells vegetable tomatoes, which he
                        sources from various locations based on the tomato
                        season. He informed us that these tomatoes are grown
                        both in greenhouses and open fields. Greenhouse
                        cultivation allows for year-round harvesting and is less
                        susceptible to diseases.
                        
                        
                        About organic vegetables:
                        Mr. Huang also discussed organic produce with us. He
                        explained that organic vegetables cannot be sprayed with
                        pesticides, which can sometimes result in less visually
                        appealing appearance. Organic farming relies heavily on
                        organic fertilizers, and the certification standards are
                        stringent, leading to higher production costs and
                        selling prices. However, as the economy continues to
                        develop, organic vegetables are gaining popularity due
                        to their environmentally friendly nature and superior
                        taste, and the consumer base for organic produce is
                        expanding.
                        
                        
                        Opinions on our treatment solution:
                        An effective green treatment approach can further
                        promote the development of organic vegetables, making
                        more people willing to invest in such produce.
                      
                
                 Ministry of Agriculture
              
              
                  
                  We visited the agricultural department and communicated with
                  government officials to learn about the regulations for
                  pesticide management and the government's views and support
                  for new technologies.
                  
                
                    Li Yonghong
                        Chief Researcher at Shenzhen Agricultural Science and
                          Technology Promotion Center
                        
                        
                        Ms. Li provided insights on tomato diseases, pesticide
                        usage, policies, and pesticide residue testing
                        regulations. She discussed biopesticides and challenges
                        in agriculture. She also explained technology adoption
                        and government innovation promotion efforts.
                        
                        
                        
                          Ms. Li's belief in biopesticides as the future of
                          agriculture aligns with our project direction. She
                          also discussed essential procedures for successful
                          implementation of new agricultural technologies,
                          offering valuable guidance for our project planning
                          and execution.
                        
                      
Li Yonghong
                        Shenzhen Agricultural Science and Technology Promotion
                        Center has primary responsibilities encompassing
                        agricultural technology advancement, crop quarantine,
                        seed management, biotechnology safety, new variety
                        promotion, and pesticide/fertilizer/seed regulations.
                        
                        
                        The Agricultural Development in Shenzhen:
                        Professor Li Yonghong informed us that agriculture in
                        Shenzhen and its surrounding areas is diverse, with a
                        focus on leafy vegetables. Various cultivation models
                        such as greenhouses and pick-your-own farms are
                        prevalent. Tomato cultivation exists in the region but
                        on a smaller scale, with a majority of pick-your-own
                        farms and small-scale farmers. Pest and disease issues,
                        including gray mold, sour rot, and powdery mildew, are
                        common, especially during the fruiting stage of
                        tomatoes. Typically, meteorological monitoring and
                        empirical predictions are used, with limited direct
                        detection methods.
                        
                        
                        Management of Pesticides by Agricultural
                          Authorities:
                        The sale and usage of pesticides are subject to
                        government regulations, with specific qualifications
                        required for pesticide sales. Different categories of
                        pesticides with varying toxicity levels are subject to
                        different management measures. The government promotes
                        the scientific use of pesticides and provides guidance
                        to farmers on the appropriate timing and methods of
                        application. Additionally, the government is concerned
                        about the impact of pesticides on soil and takes
                        measures to reduce soil pollution. For large-scale
                        cultivation, the government entrusts specialized
                        agencies to provide agricultural pest control services
                        to improve efficiency and effectiveness.
                        
                        
                        Views on Biopesticides:
                        Professor Li Yonghong mentioned that the advantages of
                        biopesticides lie in their environmental friendliness
                        and safety. However, they come with higher costs and
                        slower effects, requiring advance prediction and
                        forecasting. The government can establish certification
                        standards and collaborate with agricultural enterprises
                        to conduct large-scale cultivation experiments, using
                        data to prove the safety and effectiveness of
                        biopesticides, thereby gaining consumer trust.
                        
                        
                        Significance of Promoting Biopesticides:
                        The significance of promoting biopesticides lies in
                        meeting modern society's demands for environmental
                        protection and the quality of agricultural products,
                        with promising market prospects. Through government
                        guidance, promotion, the establishment of certification
                        standards, and collaboration with the market, consumers
                        are more willing to purchase agricultural products
                        cultivated using biopesticides.
                        
                        
                        Regarding Genetically Modified Organisms (GMOs):
                        Professor Li also shared her views on GMOs, stating that
                        GMO technology is part of technological advancement.
                        Public concerns about GMOs primarily result from
                        inadequate public awareness. GMO technology can be
                        beneficial for improving crop traits but must be used
                        correctly. In China, strict regulations and labeling
                        requirements are in place. Agricultural authorities
                        conduct GMO awareness campaigns. The approval timeline
                        for GMO crops varies depending on multiple stages, and
                        policy constraints exist.
                      
                    Ma Haifeng
                        Chief Researcher at Shenzhen Agricultural Science and
                          Technology Promotion Center
                        
                        
                        Mr. Ma shared insights into tomato diseases, their
                        treatments at the demonstration field, and discussed the
                        timing and weather conditions influencing disease
                        occurrence. He also offered on-site crop growth
                        observations, cultivation techniques, and shared his
                        experiences on plant protection concepts.
                        
                        
                        
                          Mr. Ma is optimistic about our project, viewing RNA
                          pesticides as a promising new technology. He believes
                          our plant immune-based treatment approach aligns with
                          plant protection principles and stressed the need for
                          user-friendly products, ensuring farmers' ease of use.
                        
                      
Ma Haifeng
                        Regarding the demonstration field:
                        The center oversees various agricultural functions such
                        as strategy development, policy formulation, quality
                        monitoring, disaster prevention, and crop disease and
                        pest control. Mr. Wang specializes in crop disease and
                        pest management, offering farmers technical support
                        through field observations, and possesses expertise in
                        prevalent crop diseases, pests, and control measures.
                        Mr. Ma guided us on a tour of the demonstration field
                        and explained in detail the process of breeding new
                        varieties and the implementation of demonstration trials
                        for new technologies at this location.
                        
                        
                        About plant protection:
                        Mr. Ma shared with us common tomato diseases and the
                        medications used for treatment. He also explained the
                        timing of disease occurrence and the corresponding
                        weather conditions. He led us to observe the growth of
                        some crops and planting methods on-site and shared some
                        principles and experiences related to plant protection.
                        He emphasized the importance of using a diversified
                        approach to manage plant diseases and pests, maintaining
                        ecological balance, prioritizing prevention over
                        treatment, and using pesticides rationally to achieve
                        sustainable agricultural production. Additionally, he
                        mentioned the significance of regulating pesticide use
                        in accordance with the law, improving farmers' technical
                        skills, and ensuring food safety and environmental
                        health.
                        
                        
                        Regarding our treatment plan:
                        After reviewing our treatment plan, Mr. Ma expressed
                        agreement, stating that it aligns with the principles of
                        plant protection and represents a comprehensive approach
                        to pest control.
                      
                    Wang Rifang
                        Senior Horticulturist at Boluo County Agricultural
                          and Rural Service Center
                        
                        
                        Mr. Wang emphasized that low temperatures and high
                        humidity can trigger gray mold concerns, which can be
                        mitigated through late-season planting. Triazole-based
                        pesticides are commonly used against fungal diseases,
                        but addressing resistance requires ongoing research.
                        Farmers often use experience and weather cues for
                        prevention, but disease prediction models show
                        potential.
                        
                        
                        
                          Regarding our treatment solution, Mr. Wang believes
                          that there are obstacles to enhancing immunity with
                          biopesticides due to farmer skepticism. So, proving
                          the product's effectiveness is crucial.
                        
                      
Wang Rifang
                        Tomato Production Challenges:
                        The main issues in tomato production are bacterial and
                        fungal diseases, with a focus on bacterial wilt.
                        Bacterial wilt spreads rapidly in hot and humid
                        conditions and is transmitted through the soil, leading
                        to vascular blockage and wilting.Mr. Wang mentioned that
                        grafted tomatoes are primarily used to prevent
                        soil-borne diseases such as bacterial wilt and
                        Phytophthora early blight, while diseases transmitted
                        through the air, such as late blight and gray mold,
                        still require timely control through conventional
                        planting measures.
                        
                        
                        Regarding gray mold:
                        Gray mold is a common disease in hot and humid
                        conditions. Tomatoes can be divided into early and late
                        crops, with late planting helping to reduce the impact
                        of gray mold. For fungal diseases like gray mold,
                        triazole-class pesticides are currently the main method
                        of control, providing effective and economical results.
                        
                        
                        Best Time for Pesticide Application:
                        Mr. Wang pointed out that pesticides can be applied
                        after the disease has occurred, but based on the basic
                        principles of farming, farmers typically observe
                        seedlings daily. Once a disease is detected, they
                        immediately perform appropriate pruning and apply
                        pesticides, which generally yields good results. Leaves
                        affected by the disease, usually young leaves, need to
                        be pruned because the disease can affect photosynthesis.
                        However, it's important to note that pesticides are not
                        a universal solution, and improper use can lead to
                        reduced yields. In winter, the likelihood of disease
                        infection may increase, especially under abnormal
                        weather conditions.
                        
                        
                        Regarding Disease Prediction and Warning Models:
                        Currently, disease prevention is primarily based on
                        experience and meteorological factors, with early
                        preventive pesticide use being a common practice.
                        
                        
                        About Biopesticides:
                        Currently, there is a market for immunostimulants like
                        aminooligosaccharides that enhance immunity. However,
                        farmers tend to use these products less due to the lack
                        of visible effects and cost considerations. Farmers are
                        more inclined to use treatments for diseases that have
                        already occurred and focus on effectiveness.
                        Nevertheless, from a scientific development perspective,
                        there is still a pressing demand for new technologies
                        like biopesticides. Biopesticides may have some
                        limitations compared to chemical pesticides, such as the
                        environmental and storage requirements for Bacillus
                        thuringiensis. Additionally, they require specific
                        testing parameters.
                      
                    Li Li
                        
                          Department of Agriculture and Seed Promotion Boluo
                          County Agricultural and Rural Service Center, Huizhou
                          City
                        
                        
                        
                        Ms. Li explained the process of promoting new
                        technologies with government support, involving
                        technology selection, validation, and demonstration for
                        effectiveness. Government policies offer financial aid,
                        research funding, and tax incentives. Authorities
                        recommend technologies to farmers and provide training
                        for correct adoption.
                        
                        
                        
                          Director Li emphasized the importance of both product
                          effectiveness and product user-friendliness for
                          successful promotion. She expressed her willingness to
                          support further promotion efforts if our technology
                          proves to be mature.
                        
                      
Li Li
                        The center's responsibilities encompass various aspects
                        of agricultural production, including formulating
                        agricultural development strategies, planning, and
                        policies, monitoring agricultural product quality and
                        safety, as well as disaster prevention and crop disease
                        and pest control.Ms. Li's primary responsibility
                        involves conducting on-ground trials and promoting new
                        varieties and technologies.
                        
                        
                        Regarding technology promotion:
                        Ms. Li emphasized the strong support from the government
                        for agricultural development and new technologies. He
                        mentioned that technologies like RNAi need to go through
                        a series of processes within agricultural departments,
                        including approvals and field trials, to be implemented.
                        The government actively supports the development of new
                        technologies by providing financial aid, research
                        funding, and tax incentives, among other measures.
                        During the promotion phase, relevant departments
                        introduce and provide training to farmers to ensure the
                        correct use and widespread adoption of these new
                        technologies.
                        
                        
                        As for our treatment plan:
                        Ms. Li expressed that the government highly supports the
                        use of biopesticides, which align with environmental
                        conservation principles and have substantial growth
                        potential. He emphasized the importance of the
                        treatment's efficacy, as farmers are more inclined to
                        use products that deliver good results. Additionally, he
                        highlighted the convenience of product usage as a
                        crucial factor in successful promotion. Ms.Li also
                        mentioned his willingness to assist us in further
                        promotion if our technology matures.
                      
                    Chen Wenhui
                        Director of Huizhou Boluo County Agricultural Science
                          and Technology Demonstration Center
                        
                        
                        Mr. Chen discussed pesticide sales requirements,
                        regulatory oversight, penalties, and agricultural
                        product quality and safety monitoring. He also
                        emphasized the vital role of technology in rural
                        revitalization and its significant contribution to
                        agriculture.
                        
                        
                        
                          Mr. Chen inquired about the treatment plan's
                          validation, its long-term plant protection, and
                          pesticide residues. We explained, and he stressed that
                          actual effectiveness matters most.
                        
                      
Chen Wenhui
                        The Huizhou Boluo County Agricultural Science and
                        Technology Demonstration Center's primary functions
                        include experimenting with new pesticides, introducing
                        new crop varieties and technologies, and promoting
                        agricultural research and demonstrations.
                        
                        
                        Technology dissemination plays a crucial role in rural
                        revitalization. Currently, many rural areas are
                        promoting the concept of "one village, one product,"
                        with the core idea being to unite farmers and support
                        agricultural industries to achieve prosperity in
                        villages. Continuously exploring new industrial models
                        is the inevitable choice for rural revitalization.
                        Director Chen stated that our products are a vivid
                          example of technology assisting agriculture.
                        
                        
                        Regarding pesticide usage:
                        the current situation is that most pesticides and
                        fertilizers are inorganic chemical products. Although
                        there are some organic fertilizer products available in
                        the market, their effectiveness is not ideal. Therefore,
                        many farmers prefer to purchase inorganic fertilizers
                        rather than choosing free organic ones. Consequently, we
                        may need to focus on improving the effectiveness of our
                        products, especially if the cost can be relatively high
                        while ensuring excellent results. Currently, the country
                        is actively promoting the development of organic and
                        biological pesticides, as well as organic fertilizers.
                        
                        
                        promotion:
                        Director Chen mentioned that when our products are ready
                        for practical experimentation, we can use a
                        demonstration field approach. This allows farmers to
                        witness the product's effectiveness firsthand, and they
                        can spread the word among themselves. This is considered
                        the most effective way of promotion.
                        
                        
                        
                      
                    Yin Zaobin
                        Director of Pesticide Residue Detection Center, Boluo
                          County Agriculture Bureau
                        
                        
                        Director Yin emphasized the strict requirements and
                        procedures for pesticide sales. These measures help
                        ensure the quality of agricultural products and
                        safeguard consumer health.
                        
                        
                        
                          Director Yin highly appreciates our biopesticide,
                          seeing great potential in it due to its approximately
                          30% profit margin in the pesticide industry. Effective
                          results and farmer trust are essential for success,
                          and biopesticides offer a safer alternative to
                          chemical pesticides.
                        
                      
Yin Zaobin
                        The main responsibility of Boluo County Agriculture
                        Bureau's Pesticide Residue Detection Center is to test
                        pesticide residues to ensure the quality and safety of
                        agricultural products, thereby ensuring compliance with
                        regulations and consumer health. We would like to
                        inquire with Director Yin about the current pesticide
                        residue detection standards and sampling criteria.
                        
                        
                        Views on biopesticides:
                        The teacher believes that biopesticides offer greater
                        safety. For example, while herbicides like Paraquat are
                        highly effective, they pose significant health risks to
                        humans. Consequently, liquid formulations have been
                        adjusted to have an irritating odor to prevent misuse.
                        However, these measures have not completely eliminated
                        incidents of self-harm, leading to a gradual phase-out.
                        In contrast, our biopesticides have promising prospects.
                        Unlike fertilizers and other products, our pesticides
                        still hold high value.
                        
                        
                        Requirements for pesticide sales:
                        Pesticide sales require a license and compliance with
                        various conditions and training. Purchasing pesticides
                        involves identity registration through a specialized
                        platform. Licenses typically need to be renewed every
                        five years, and past penalties may necessitate
                        reapplication. Additional requirements may include
                        maintaining records, township-level inspections, and
                        regular enforcement checks.
                      
                
                 Consumers
              
              
                  
                  We communicated with consumers at supermarkets to understand
                  their consumption habits and their views and attitudes towards
                  pesticide residues. In the process, we also discovered that
                  consumers have misconceptions about new technologies. This has
                  prompted us to carry out educational campaigns to eliminate
                  these misunderstandings.
                  
                
                    Ms. Guo
                        28 years old
                        
                        
                        Ms. Guo mentioned that younger consumers prioritize
                        vegetable and fruit prices and convenience, while older
                        consumers are more concerned about food safety and
                        quality, especially pesticide residues.
                        
                          These differing considerations affect their purchasing
                          choices, inspirating the importance of surveying
                          different age groups.
                        
                        
                        
                        We also introduced our biopesticide to Ms. Guo. She
                        expressed that she would be very willing to purchase
                        such products if they didn't significantly raise the
                        prices of agricultural products and if they improved the
                        taste of the produce.
                      
Ms. Guo
                        Ms. Guo was randomly interviewed during our visit to the
                        Dabanqiao Market in Nanshan District, Shenzhen.
                        Dabanqiao Market is a daily market where local residents
                        purchase vegetables and fruits. It is home to more than
                        100 vendors.
                        
                        
                        Shopping Habits:
                        The individual usually uses Meituan for grocery shopping
                        but decided to visit the farmer's market on this
                        occasion for its convenience and cost-effectiveness.The
                        shopper evaluates groceries based on factors like value
                        for money, pricing, and the taste of the products.
                        
                        
                        Priorities:
                        There is no specific emphasis on organic or
                        pesticide-free produce during this visit. The primary
                        concern is to make practical and budget-conscious
                        purchases.
                        
                        
                        Age Differences:
                        The shopper acknowledges that different age groups may
                        have varying priorities. Older individuals may place
                        more importance on pesticide residues in groceries but
                        will still consider price when making their purchases.
                        
                        
                        Inspriration:
                        This also provides us with a valuable insight, which is
                        to pay special attention to the perspectives and needs
                        of different age groups when conducting consumer
                        surveys. In our future consumer surveys, we have been
                        mindful of asking about their age to observe the focus
                        areas of various age demographics.
                      
                    Ms.Lin
                        40 years old
                        
                        
                        Ms. Lin expressed that pesticide residues and similar
                        concerns should be addressed by market regulations
                        rather than consumers. She mainly considers
                        affordability and freshness when shopping for groceries.
                        She has concerns about genetically modified crops and
                        avoids purchasing them. As for organic produce, she is
                        not very familiar with it since it's only available in a
                        few expensive supermarkets, and she doesn't actively
                        seek it out.
                        
                        
                        We introduced the concept of biopesticides to Ms. Lin
                        and explained that our products don't carry the risk of
                        genetic modification.
                      
Ms.Lin
                        We met Ms. Lin during a random interview at the
                        Dabanqiao Market in Nanshan District, Shenzhen. This
                        market is where local residents buy groceries and has
                        over 100 vendors selling fruits and vegetables.
                        
                        
                        Primary Purchase Considerations:
                        Ms. Lin mentioned that she typically doesn't include
                        factors related to pesticide residues and similar issues
                        in her purchasing decisions, as she considers it the
                        responsibility of market regulation. When selecting food
                        items, she places greater emphasis on affordability and
                        the freshness of the ingredients. Regarding genetically
                        modified crops, she usually opts not to purchase them
                        due to concerns.
                        
                        
                        View on Organic Food:
                        Ms. Lin believes that organic food is relatively
                        expensive. She has limited knowledge about the benefits
                        of organic food and may perceive no significant
                        difference in taste and quality compared to regular
                        food. As a result, she is not very inclined to purchase
                        organic food.
                      
                    Mr.Qiu
                        62 years old
                        
                        
                        Mr.Qiu believes that market regulation is necessary for
                        pesticide residue issues. He thinks it's difficult for
                        consumers to know the pesticide usage on the vegetables
                        they consume. He believes that there are many
                        genetically modified fruit tomatoes in the market(which
                        is actually a misconception on his part) and worries
                        about the presence of genetically modified organisms.
                        
                        
                        After hearing about our product, Uncle Qiu expressed
                        interest.His misconceptions about genetically modified
                        products in the market also motivated us to consider
                        providing education to address consumer misconceptions.
                      
Mr.Qiu
                        We met Mr.Qiu in Huaren Wanjia, a large chain
                        supermarket in Shenzhen known for its rigorous
                        inspection process that ensures the quality and safety
                        of produce.
                        
                        
                        Attitude towards pesticide residues:
                        Mr. Qiu believes that the issue of pesticide residues
                        requires the support of market supervision because
                        consumers often find it difficult to proactively select
                        vegetables that meet safety standards. They lack
                        relevant information and struggle to determine whether
                        the vegetables they purchase are affected by pesticide
                        residues. Therefore, he believes that promotion and the
                        establishment of relevant standards are crucial.
                        
                        
                        Attitude towards GMOs:
                        Holds a skeptical attitude towards genetically modified
                        organisms (GMOs) and has misconceptions, believing that
                        most tomatoes in the market are genetically modified
                        
                        
                        Inspiration:
                        Mr. Qiu's misconceptions have made us realize the lack
                        of knowledge and understanding among the public
                        regarding new technologies. To dispel public doubts and
                        misunderstandings about new technologies, especially
                        when it comes to emerging technologies, scientific
                        outreach becomes crucial. This underscores the need for
                        broader dissemination of relevant knowledge to ensure
                        that people have a correct understanding of new
                        technologies and are better equipped to accept and apply
                        them.
                        
                        
                        
                        
                      
                    Ms.Fan and Ms. Li
                        73 years old
                        
                        
                        Huaren Wanjia, a prominent Shenzhen supermarket chain,
                        is renowned for its rigorous inspection of fruits and
                        vegetables, offering consumers greater confidence in
                        quality and safety. We interviewed two shoppers.
                        
                        
                        They usually buy groceries at the local market because
                        it's fresher and more delicious, and they occasionally
                        come to the supermarket. She believes that pesticide
                        residue issues are a matter for government regulation
                        and not something the consumers can control or address.
                        As for organic vegetables, they currently find it
                        difficult to buy organic produce as there aren't many
                        organic options available in the market yet.
                      
Ms.Fan and Ms. Li
                        Huaren Wanjia, a large chain supermarket in Shenzhen, is
                        known for its rigorous inspection process for the sale
                        of fruits and vegetables, providing consumers with more
                        assurance. This strict inspection process helps ensure
                        the quality and safety of fruits and vegetables. We
                        interviewed two madams who are currently shopping for
                        groceries here.
                        
                        
                        Regarding pesticide residues:
                        both grandmothers believe that market regulatory
                        authorities should ensure the safety levels of pesticide
                        residues in food. They think that consumers often find
                        it difficult to determine on their own whether
                        vegetables and fruits are affected by pesticide
                        residues, and therefore, regulatory oversight and
                        standards from relevant authorities are needed to
                        guarantee safety.
                        
                        
                        Shopping habits for groceries:
                        The grandmothers mentioned that they usually buy
                        groceries at the local market because they find the
                        produce to be fresher and more delicious. Occasionally,
                        they may visit a supermarket for their shopping
                        needs.
                      
                    Mr. Xu
                        23 years old
                        
                        
                        Mr. Xu, a supermarket employee, is not highly concerned
                        about pesticide residues and generally trusts government
                        regulations. He mentioned that he doesn't typically
                        purchase organic vegetables due to their higher prices.
                        
                        
                        
                          After listening to our biological pest control
                          solution, he expressed a positive attitude and said he
                          is open to new technologies and approaches. However,
                          he mentioned that older consumers may not be as
                          receptive.He told us that it's essential to educate
                          the public more about new technologies to dispel
                          misunderstandings and concerns.
                        
                      
Mr. Xu
                        Regarding agricultural products:
                        Mr. Xu personally doesn't pay much attention to
                        pesticide residues and has trust in government
                        regulatory mechanisms. As for organic vegetables, he
                        doesn't typically purchase them because of their higher
                        prices.
                        
                        
                        We explained our treatment plan to Mr. Xu, and he
                          responded with a positive attitude.
                        He expressed his willingness to embrace new technologies
                        and methods, b elieving that by introducing these
                        innovations, better results can be achieved, efficiency
                        can be improved, or existing challenges can be
                        addressed. This positive attitude is often instrumental
                        in driving innovation and development, leading to the
                        success of projects or programs.
                        
                        
                        
                          reflection:Mr. Xu believes that the younger generation
                          is more likely to embrace new technologies, but he
                          also thinks that older consumers may not necessarily
                          be willing to accept them. This underscores the
                          importance of proactive communication and addressing
                          concerns when promoting new technologies. In our
                          future consumer surveys, we will actively explain the
                          principles of our technology and considerations
                          related to safety to consumers.
                        
                      
                    Ms. Qin
                        40 years old
                        
                        
                        Ms. Qin expressed her strong concern about pesticide
                        residues and her preference for purchasing vegetables
                        from large supermarkets, which she believes offer better
                        quality control. While she's interested in organic
                        vegetables, she doesn't buy them regularly due to their
                        higher prices, and she is not very open to genetically
                        modified products.
                        
                        
                        
                          After introducing our biological pest control
                          solution, Ms. Qin expressed optimism about our
                          product. She hopes that adopting biological pest
                          control won't significantly raise vegetable prices and
                          stressed the importance of effective regulation and
                          safeguards for new technologies.
                        
                      
Ms. Qin
                        Regarding pesticide residues:
                        Ms. Qin is very concerned about pesticide residues and
                        their potential impact on food safety and health,
                        especially for children and the elderly. Faced with this
                        concern, she tends to prefer buying organic food or
                        products labeled as pesticide-free because she believes
                        these products meet her requirements for food safety
                        
                        
                        In addition, Ms. Qin is more inclined to shop at large
                        supermarkets because she believes that these
                        supermarkets typically have stricter quality assurance
                        measures and can provide more reliable food supplies.
                        
                        
                        Regarding our biological control solution:
                        Ms. Qin remains optimistic about our product. However,
                        she expresses a concern that adopting biological control
                        methods should not significantly increase the prices of
                        vegetables. She also emphasizes the importance of having
                        reliable oversight and safeguards in place for new
                        technologies. This indicates her desire for a balance
                        between adopting innovative techniques and ensuring
                        affordability and safety in the agricultural sector.
                      
Improvement and interaction
Reflection
                  
                  After in-depth discussions with various stakeholders related
                  to tomatoes, we discovered that different stakeholders had
                  varying concerns regarding the pesticide. These made us
                  realize that we had not adequately considered these needs
                  during the early stages of project design!
                  
                  
                
After thoroughly understanding the needs and priorities of the stakeholders involved in the tomato industry, we carefully analyzed and evaluated our project. Based on the valuable feedback received from stakeholders, we realized the importance of making specific improvements and adjustments to align with their values and requirements.
So we improved and adjusted our project to meet with the values and need they are prioritizing. After understanding the needs of the stakeholders involved in tomatoes, we made improvements to our project in five key areas to meet their requirements.
              Responsiveness
"More effective"
Overcoming bottlenecks to improve effectiveness
                      
                      Agricultural Producers:
                      
                      Mr. Cheng:
                      
                        "I believe the most important thing about pesticides is
                        their effectiveness. If pests and diseases disappear
                        after using pesticides, we are happy."
                      
                      
                      Mr. Liang:
                      
                        "Chemical pesticides nowadays develop resistance after
                        just a few applications. I hope we can have effective
                        pesticides."
                      
                      
                      Mr. Hong Chuangjiang:
                      
                        "We also use some biopesticides, but they take longer to
                        show results. I hope biopesticides in the future will
                        work faster and be more effective."
                      
                    
                    
                    
                      
                      Ministry of Agriculture :
                      
                      Researcher Ma Haifeng:
                      
                        "For your product, you must have ample experimental data
                        to prove that your treatment is better than other
                        pesticides. Only then will farmers trust your product,
                        and the agricultural department will promote it."
                      
                      
                      Director Chen Wenhui:
                      
                        "The most important thing about pesticides is whether
                        they work well or not. If they don't work well, farmers
                        won't be willing to use them."
                      
                    
                      "Agro-Knowledge Conference"
                      
                      "Agro-Knowledge Conference," themed "Future Pesticides
                      Driven by Cutting-Edge Technology, Leading a New Journey
                      of Green Innovation," brought together experts,
                      representatives from the pesticide industry, academia,
                      research, and capital. Distinguished figures like Lv
                      Yuping, General Manager of Longping Bio, Professor Yang
                      Guangfu from Huazhong Normal University, Tang Xueming,
                      Chairman of Silicon Arrow Technology and so on were
                      invited to
                      
                        collectively explore the commercialization of future
                        pesticide technologies driven by cutting-edge
                        advancements.
                      
                      
                      
                      Our team participated in this conference and learned about
                      the three main bottlenecks in the commercialization of RNA
                      pesticides:
                      
                      
                    
- 
                        1. RNAi molecules may not efficiently enter target
                          cells and function.
                        
2. RNAi may have weak target effects.
3. The production of RNAi molecules relies heavily on cell fermentation, making it difficult to reduce costs. 
                      
                    
                
                  To make our treatment solution more effective and meet the
                  expectations of producers and the agricultural department, we
                  proposed our own solution to improve the efficient entry of
                  RNAi molecules into target cells, as well as reducing the
                  potential for off-target effects and providing a more
                  sustained effect.
                  
                  
                
                  The figure explains the principles of CPP (the picture was drawn by YiXi Wang)
                  Using CPP (Cell-Penetrating Peptides) to improve delivery
                    efficiency:
                  We employed KH9-BP100 (a CPP) combined with shRNA. By spraying
                  CPP-shRNA onto tomatoes infected with B. cinerea,
                  shRNA, with the assistance of CPP, can enter plant cells more
                  efficiently. This enhances the delivery efficiency of
                  biomolecules into plant cells.
                  
                  
                  Bi-ShRNA providing a more sustained effect:
                  However, by using specific sequences to link shRNAs targeting
                  different mRNAs, the resulting bi-shRNA molecules can be
                  processed into various siRNAs, each with a distinct function.
                  This approach allows for a lower effective dose compared to
                  traditional siRNA/shRNA, reducing the potential for off-target
                  effects and providing a more sustained effect.
                  
                
Please refer to the detailed content in Proof of Concept and Engineering success
"Safer"
Multiple Measures to Ensure Safety
                      Ministry of Agricultural :
                      
                      Researcher Li Yonghong:
                      
                        "Chemical pesticides nowadays have a significant
                        environmental impact. Your product must ensure that it
                        doesn't have any adverse effects on the environment."
                      
                      
                      Minister Yin Zaobin:
                      
                        "One of the reasons for the discontinuation of paraquat
                        is the high number of suicides by consuming pesticides.
                        A good product should consider its safety for human
                        health."
                      
                      
                      Horticulturist Wang Rifang:
                      
                        "The government is currently advocating for
                        environmental protection. Environmental safety, human
                        health, and the absence of pesticide residues are all
                        aspects your product needs to excel in."
                      
                    
                    
                    
                      
                      Consumers:
                      
                      Ms. Qin:
                      
                        "I usually buy groceries from large supermarkets for
                        some added assurance. I'm particularly concerned about
                        pesticide residues exceeding the limits and also worry
                        about genetically modified products."
                      
                      
                      Mr. Qiu:
                      
                        "We don't know how many pesticide residues are in the
                        vegetables available in the market. It's a big concern.
                        I also avoid purchasing genetically modified foods."
                      
                      
                      Mr. Xu:
                      
                        "I'm quite optimistic about biopesticides. They are more
                        environmentally friendly and harmless to humans."
                      
                    
                  
                    Everyone is deeply concerned about environmental safety and
                    human health. We also considered safety-related issues
                    during the initial project design. It's for safety reasons
                    that we chose RNA interference (RNAi) and spray-induced gene
                    silencing (SIGS) using exogenous dsRNA for treatment, which
                    have the following safety advantages:
                    
                    
                  
- 
                      1.Specific Targeting:
                      It exclusively targets and silences essential genes of
                      B. cinerea, causing no harm to other organisms.
                      
2.Environmental Friendliness: RNAi molecules are easily degraded, making them environmentally friendly and free from pesticide residues.
3.Non-GMO: Unlike genetic modification technologies, RNAi-based biopesticides do not result in permanent changes in plant gene expression and are controllable. 
                      
                    
                      Choosing Environmentally Friendly
                      Bacillus subtilis
                      :
                    
                    In triggering plant immunity, we selected Bacillus subtilis
                    as the carrier to express immune-inducing factors. Bacillus
                    subtilis is one of the most widely accepted plant probiotics
                    and is environmentally friendly.
                    
                    Constructing a Suicide Switch for Safety:
                    To prevent our engineered bacteria from spreading and
                    surviving in the fields, we designed a suicide switch to
                    further ensures the safety of the product in the
                    environment.
                  
Please refer to the detailed content in Design
"More Affordable"
Cost Reduction through Automation Hardware
                      
                      Agricultural Producers:
                      
                      Mr. Liang:
                      
                        "We are concerned about pesticide prices. If they are
                        too expensive, us farmers won't be able to afford them."
                      
                      
                      Mr. Cheng:
                      
                        "For this over 100-acre land, one pesticide spray costs
                        me around 4,000 RMB. This price is acceptable, but if
                        pesticides become too expensive, it won't be
                        cost-effective."
                      
                      
                      Mr. Wang
                      
                        "Pesticides are essential, and we use them a lot, so I
                        usually don't choose expensive ones."
                      
                      
                      
                    
                    
                    
                      
                      Tomato Sellers:
                      
                      Mr. Cheng Jiabo:
                      
                        "As business owners, we are quite price-sensitive. If
                        your pesticides are too expensive, it raises production
                        costs, which can impact the overall profitability of our
                        agricultural operations. Therefore, we prefer pesticides
                        that strike a balance between effectiveness and
                        affordability, as long as they meet our needs and are
                        accepted by consumers."
                      
                      
                      Mr. Wang:
                      
                        "Pesticides should primarily be practical and
                        affordable, not too expensive. If they are accepted and
                        recognized by the general public, we are willing to
                        accept them. What we sell also depends on what consumers
                        like."
                      
                    
                  
                    
                    
                      Price is a concern for both farmers and sellers. In the
                      early stages of development of RNA pesticide, our product
                      cannot be as inexpensive as chemical pesticides. However,
                      we aim to reduce production costs as much as possible to
                      make eco-friendly and affordable products accessible to
                      everyone. Therefore, we have designed hardware to explore
                      the possibility of large-scale production cost reduction.
                    
                    
                  
                      
                    
                    An Automated Equipment for Large-Scale Production of
                      shRNA Molecules through a Cell-Free Method:
                    We designed and constructed an automated hardware system
                    suitable for large-scale synthesis of shRNA molecules. The
                    automated production process of shRNA molecules is
                    established by programming the microcontroller. The system
                    can be operated by non-scientists and can be applied in
                    non-laboratory environments. It aims to improve the
                    convenience of shRNA production while reducing associated
                    costs and opening up possibilities for further expansion of
                    production.
                  
Please refer to the detailed content in Hardware
"More User-Friendly"
Polycobead - Easy Application for Effective Disease Control
                      Ministry of Agricultural :
                      
                      Minister Li Li:
                      
                        "Your product must be suitable for farmers' use, simple,
                        easy to use, and easy to store. This is crucial for
                        widespread adoption."
                      
                      
                      Researcher Li Yonghong:
                      
                        "Some pesticides can be very complicated to use, which
                        discourages farmers and may lead to misuse. It would be
                        better if your product is designed to be straightforward
                        for farmers to accept."
                      
                      
                      Researcher Ma Haifeng:
                      
                        "User-friendliness is essential. How do you plan to
                        ensure farmers can use the two formulations in your
                        project? A simpler design might be better."
                      
                    
                    
                    
                      Agricultural Producers:
                      
                      Mr. Cheng:
                      
                        "Pesticide application can be really troublesome. Every
                        time, I have to request technicians to help. Some
                        pesticides can't be mixed, or they become ineffective
                        when mixed. It's also a hassle to calculate the right
                        proportions. Often, I'm not sure how much to add, and
                        it's a real headache."
                      
                      
                      Mr. Liang:
                      
                        "Mixing pesticides is indeed a technical task. Every
                        time, I have to spend a lot of time reading the
                        instructions, and there are many precautions. It's
                        really cumbersome."
                      
                      
                      Mr. Hong Chuangjiang:
                      
                        "Sometimes, I don't even know how to mix pesticides, so
                        I also hope that pesticide application can be made
                        simpler and more convenient."
                      
                    
                
                  
                    
                    In our communications with farmers, we found widespread
                    pesticide misuse in the agricultural sector.
                  
                  Firstly, some farmers apply pesticides excessively, deviating
                  from recommended dosage, which leads to overuse and
                  environmental pollution. Secondly, farmers are often uncertain
                  about how to correctly mix and use multiple pesticides,
                  resulting in losing effectiveness.
                  
                  
                  
                  To address these challenges and make pesticide application
                  more user-friendly, we drew inspiration from laundry detergent
                  beads. We decided packaging our product in a form with fixed
                  dosage , encapsulating it within a single bead. We would then
                  provide guidance to farmers on the number of beads for
                  treating and preventing tomato gray mold disease.
                  These lead to the design of our product -- POLYCOBEAD.
                
                        
                      
                        
                      Polycobead uses polyvinyl alcohol (PVA) to encapsulate our CPP-shRNA formula and engineered Bacillus subtile by simply placing the designed small beads in a specific volume of water. They dissolve quickly, allowing direct field spraying. It has the following advantages:
- 
                        
1.Ease of Use and Precise Application: The drug preparation process in Polycobead is accurately mixed in accordance with scientific proportions, without complex preparation or farmer mixing. Dissolve the beads in water and use them.
2.Extended Shelf Life: the liquid environment inside the bead contains glycerin, which helps to extend the storage life.
3.Environmentally Friendly: The PVA film selected by Polycobead has good biocompatibility and degradability. No harm to the environment. 
Please refer to the detailed content in Engineering
"More Precise"
Accurate Modeling for Targeted Spraying
                      Agricultural Producers:
                      
                      Mr. Cheng:
                      
                        "There's a problem that troubles me. You see, I manage
                        over 100 acres of land. If I find gray mold disease in
                        one area, how large an area should I spray with
                        pesticides to prevent its spread? Spraying the entire
                        100 acres is costly, but spraying too little might not
                        effectively prevent it."
                      
                      
                      Mr. Hong Chuangjiang:
                      
                        "Usually, when I discover a disease, I have no choice
                        but to spray pesticides across the entire field to
                        ensure prevention, even though it can be quite
                        expensive."
                      
                      
                    
                    
                    
                      Ministry of Agriculture:
                      
                      Li Yonghong:
                      
                        "Agriculture is increasingly shifting toward large-scale
                        farming, but it also poses some challenges. For
                        instance, on several hundred acres of land, when a
                        disease occurs in a particular area, how much pesticide
                        should be sprayed to effectively control it?"
                      
                      
                      Mr. Chen Wenhui:
                      
                        "Even in my demonstration field, we sometimes encounter
                        diseases, often limited to a specific region. Without
                        timely control, the disease can quickly spread to every
                        region."
                      
                    
                
                  
                  In response to these issues, we've developed two models to
                  effectively guide the practical application of Polycobead:
                  
                  RNAi Dosage Optimization Model:
                  
                  By applying optimal control and optimization theories, we
                  optimize the cost function, guiding farmers to use RNAi
                  pesticides optimally for disease control, product quality, and
                  yield while minimizing costs.
                  
                  
                  Pesticide Application Range Model:
                  
                  This model simulates disease development patterns under field
                  conditions to predict the effective application area and
                  quantity of pesticides required when diseased plants are
                  discovered. Its aim is to minimize costs and environmental
                  impact while effectively controlling disease spread.
                
Please refer to the detailed content in Model
                      RNAi Dosage Optimization Model
                    
                      Pesticide Application Range Model
                    Responsibility
                
                  Our team attaches great importance to safety and
                  responsibility, so we have taken a series of measures to
                  ensure that our POLYCOBEAD product complies with legal and
                  societal requirements. We have carefully analyzed the legal
                  framework concerning the use and production of microbeads.
                  Additionally, we actively engage with communities,
                  environmental organizations, and consumer groups to address
                  their concerns and educate them about our solutions. Through
                  these measures, we ensure that our POLYCOBEAD product not only
                  meets legal requirements but also aligns with societal
                  expectations and values. 
              
Legal Analysis
                
                During discussions with Chief Researcher Li Yonghong from the
                Shenzhen Agricultural Promotion Center in China, we learned that
                expanding the scale of product testing during the trial phase
                and bringing a product to market require compliance with
                relevant laws and policies. This includes ensuring the safety of
                trials, adherence to testing standards, and consideration of the
                product's safety and compliance with health and environmental
                standards. These regulations and policies are crucial for the
                commercialization of technology and industrial development.
                
                
                
                  Therefore, we wrote a document titled "Legal Analysis -
                  Investigation of Polycobead Application under Existing Laws"
                  to conduct a thorough examination of relevant regulations and
                  policies. This was done to clarify the legal standards that
                  our product needs to adhere to, particularly in the context of
                  emerging technologies like RNAi and novel plant immune agents.
                  This effort has laid a robust legal compliance foundation for
                  the future development of our product, Polycobead.
                
              
The Proposal of Chinese RNA Pesticide Industry Standard
                Our product is initially planned to ernter the market in China.
                However, we discovered that China currently lacks clear
                classification and standardized regulations for RNA pesticides.
                This highlights significant legal and regulatory challenges in
                the RNA pesticide sector in China, emphasizing the urgent need
                for applicable regulations and standards to ensure safety and
                sustainability in this emerging field.
                
                  
                  
                  To avoid misuse of RNA pesticide, we develop sufficient
                  countermeasures to mitigate such risk by putting forward --
                  "The Proposal of Chinese RNA Pesticide Industry Standard "
                
              
Collaboration and Guidance from expert
                    
                    
                
                  After drafting the "Proposal for RNA Industry Standards in
                  China," we proactively reached out to Ms. Li Yonghong and
                  Dr.Jin, the researchers at the Shenzhen Agricultural Science
                  and Technology Promotion Center, seeking further guidance and
                  advice. Ms. Li Yonghong expressed a positive response to our
                  initiative and believed that our proposal has the potential to
                  effectively promote the development of regulations and
                  standards in the field of RNA pesticides in China.
                  
                  
                
                Ms. Li Yonghong outlined the steps required to advance our
                proposal. First, we must submit it to the Market Supervision
                Administration and the Shenzhen Standards Promotion Association
                for review and approval. We'll then collaborate with industry
                experts to gain recognition. Next, we'll initiate the standard
                declaration process, including expert review and certification.
                Finally, we'll gather public and business feedback for
                widespread acceptance.
                
                
                We will continue to work within this framework, driving the
                development of regulations and standards in the field of RNA
                pesticides in China .
              
T.O.M.A.T.O Alliance
                    "how do we balance the interests
                    
                    between our project and the communities negtively impacted"
                  
                  The emergence of new products and technologies will inevitably have an impact on the traditional pesticide industry, affecting the interests of them. We noticed that chemical pesticides still dominate the majority of the pesticide market. Considering their interests and for the stable development of the pesticide industry, we have established the T.O.M.A.T.O-Technical Alliance (the Technology-Oriented Modernization of Agriculture-Targeted Organization). Through technology sharing and experience exchange, we aim to lead traditional enterprises in transforming and upgrading, avoiding unnecessary market competition and minimizing conflicts between new and old products to achieve mutual progress.
                      
                      In addition to the product technology alliance, we propose the concept of the T.O.M.A.T.O-Thought Alliance (Thought-Oriented Modernization of Agriculture-Targeted Organization) . Because expect to the market aspect, the successful implementation and widespread adoption of the products also rely on the involvement of various stakeholders such as farmers, consumers and government. Farmers directly determine its sales, consumers' attitudes impact the market, and governments' policies have a significant influence on the development path of the products. Therefore, establish a Thought Alliance is meaningful. We hope to rally the power of knowledgeable individuals from various sectors to promote awareness of using biopesticides. Besides focusing on product and market aspects, we aim to raise people's attention towards green agriculture and ecological protection, forging ahead towards a blueprint for sustainable agriculture.
Please refer to the detailed content in Entrepreneurship
Popular science summer camp
Through conversations with consumers, we've found that many have concerns and misunderstandings about new technologies due to a lack of understanding. This led us to realize that for Polycobead to be widely accepted, we need to explain the principles of the new technology and educate consumers to dispel their concerns and misconceptions.
                  
                  
                
                
                As a response, we organized an educational summer camp where we
                explained the principles of RNA pesticides and plant immunity to
                consumers, addressing their misconceptions and helping them
                understand Polycobead's new technologies and advantages.
                
                  In a subsequent survey, the majority of consumers showed
                  significant interest and a willingness to support our product.
                  This consumer affirmation positions our product for future
                  wide use and acceptance.
                
              
                  
                  Implementation and imagination
Close Our Loop
"Revisiting Dacheng Xin Tomato Farm"
              
              
              In late September, as tomato seedling cultivation began at Dacheng
              Xin Tomato Planting Base, our experiments gained initial success.
              At this pivotal juncture, we revisited the farm in Shanwei City
              and engaged in in-depth discussions with Mr. Cheng, Mr. Cheng, who
              owns over 200 acres of tomato fields, and Mr. Fan, who is
              responsible for sales.
              
              
              
                We introduced our Polycobead product's curing strategy and
                designing concept at the tomato planting base. We emphasized
                Polycobead's advantages, highlighting its environmentally
                friendly, residue-free biocontrol measures for effective gray
                mold control. We also stressed its user-friendliness, enabling
                precise application with no need for complex preparation or
                mixing,.
              
              
            
            
              
                After our explanation, Mr. Cheng expressed strong interest in
                Polycobead. He considered it was a convenient, precise, and
                environmentally friendly solution to the grey mold controlling,
                which is also capable of controlling the situation of ovsrusing
                pesticide.
              
              
              
              
                Mr. Fan emphasized the intense market competition and consumers'
                high demands for food quality and safety. He believed that
                Polycobead could enhance tomato quality, reduce pesticide
                residues, and increase their product's competitiveness.
              
              
              
              After hearing their feedback, we were greatly encouraged to know
              that our product has the potential to address the challenges they
              face and bring benefits to their agricultural production. We will
              continue to work diligently to bring Polycobead to the market as
              soon as possible, meeting everyone's needs and our mutual goal for
              sustainable agriculture.
            
From Unit to Union
After conducting legal research and standard formulation, we have created an orderly market environment for polycobead. Then, we assessed the development potential of POLYCOBEAD in a long run from both market and societal perspectives. We made a business plan and strategic formulation, proposing the "From Unit to Union" development plan. Starting from relevant units, we aim to connect each group and form a unified union—T.O.M.A.T.O Alliance.
            
              
              Firstly, we analyze the interests, needs, and backgrounds of each
              unit, examining the acceptance of different groups towards
              polycobead.
              
              
              After gaining a comprehensive understanding of each unit and the
              pesticide industry, we unite each group to form the T.O.M.A.T.O
              Alliance, including Technology-Oriented Modernization of
              Agriculture-Targeted Organization (Technical Alliance) and
              Thought-Oriented Modernization of Agriculture-Targeted
              Organization (Thought Alliance).
            
            
              
              The emergence of new products is likely to pose a threat to the
              traditional chemical pesticide industry. Therefore, the
              establishment of the T.O.M.A.T.O Technology Alliance aims to avoid
              conflicts between new and old products in the market competition.
              We call for advanced biotechnology companies to join the research
              and production of biopesticides and to encourage traditional
              enterprises to undergo transformation and upgrading.
              
            
            New products and technologies will inevitably face many cognitive challenges in the process of entering the market. Therefore, we have established the T.O.M.A.T.O Thought Alliance, uniting each unit, engaged in science communication and provided proposal suggestions. We aim to improve the awareness of all units involved, clearing obstacles for the promotion of products and the advancement of Green and Sustainable Agriculture.
Please refer to the detailed content in Entrepreneurship
Summary
                Proposed by our team SZU-China, the “5I” cycle
                is bound to provide the guidance for other teams.
                
                
                Under the guidance of the 5I principle,
                we completed the closed loop of Human Practice work.
                We fully improved the feasibility of the project in the process
                and delivered our own value.
              
              
                
                
                  "what we are doing, why we are doing it, how the world might
                  affect our work, and how our work might affect the world."
                
                
              
Now we are sufficient to answer these question!
We learned about the harm of gray mold to the agricultural industry from the field inspection of the Dacheng Xin Planting Base, and continuously investigated the needs and attitudes of stakeholders during the process of project improvement , and made "5I Principle" to meet the actual needs . And finally generate our product —— POLYCOBEAD.
At the same time, we pay attention to the connection and impact between products and the world , and convey our values and attitudes of safety and responsibility through laws and regulations and industry standards.
Then, we visited the tomato base again, investigated the feasibility of the product at the actual production site , collected user attitudes, and popularized the product concept.
Through the detailed investigation of the whole process of the stakeholders involved in the use of pesticides, we put forward the concept -- From Unit to Union . We formed the TOMATO Technology Alliance , which balance the interests between traditional pesticide companies and advanced biotech companies. At the same time, we have formed the TOMATO Thought Alliance , which unites people from all walks of life to improve recognization of the product and raise awareness of sustainable agriculture .
The names and photos of the citied individual are used with their specific permission and informed consent.