First, we strictly abide by the corresponding laboratory safety rules and formulate a series of specific measures and systems to ensure the progress of experiments and the safety of personnel. The rules are as follows:
    1) When entering the laboratory, strictly follow all safety guidelines.
    2) Safety training must be conducted before any laboratory work. Care should be taken to avoid accidents with personnel or instruments during laboratory work.
    3) You must always wear lab clothes in the laboratory.
    4) Laboratory work and data recording must be carried out scientifically.
    5) When using the instrument, strictly abide by the agreement. In case of abnormality, stop the instrument immediately and report it to the instrument expert/technician.
    6) The acquisition, storage, and treatment of biochemical reagents must be strictly supervised.
    7) After completing the experiment, clear the lab space and put back all tools.
    8) Experiments without operating schemes are not allowed.
    9) Experiments without security risk analysis are not allowed.
    10) If the operator is not properly protected, the experiment is not allowed.
    11) Experiments without field processing protocols are not allowed if field processing procedures are required for any experiment.
    12) Experiments are not allowed if there is no on-site processing protocol.
    13) Under the supervision of fewer than two persons, potentially dangerous experiments (i.e. High temperature, high pressure, and high speed) are not allowed.
    14) No night experiments are allowed under the supervision of less than two people.
    15) Experiments involving hazardous gases (toxic, explosive, and flammable) are not allowed without proper ventilation, explosion-proof power supply, and monitoring alarms.
    16) Experiments are not allowed if the operator is not aware of the MSDS (Material Safety Data Sheet).
    17) If the operator has not received safety training (safety training includes: operating procedure description, risk analysis notification, use of protective equipment, etc.), the experiment is not allowed.

    Second, we will also benefit from the training we have received to manage potential risks in experiments. In addition to training in laboratory visits and rules, other training is also very helpful. For example, after learning the rules for transporting samples between labs or between institutions, we will have a more comprehensive consideration before exchanging parts or materials with other teams. In March, team members were tested on basic safety guidelines for experiments. In April, they were trained in the laboratory on how to deal with emergencies. In May, our team members learned about safety knowledge policies from the iGEM website.

    Third, our experimental equipment and space are enough to carry out the experiments of our project. For example, when transfection is performed, our laboratory can provide a sterile environment to ensure the safety of personnel and the success of experiments.
    Fourthly, the biosafety level of the laboratory where we work meets the second-class biosafety standard (BSL-2).

    Fifthly, our former team members gave us laboratory safety training at the meeting, and made clear the laboratory use specifications. We have studied molecular experiments and microbiology experiments.
    Sixth, all the biological waste was put in the Bio-Hazard bag, which then was collected by the safety department of our university.
    Seventh, our laboratory has a computer without networking, which is specially used to record and analyze experimental data.

The molecular experiments in our project mainly use safety standard kits and non-toxic reagents as much as possible. We will comply with all laboratory disciplines and academic standards, and conduct all experiments under guidance and safety precautions, including wearing experimental clothes, gloves and masks throughout the experiment, conducting electrophoresis in designated areas, and washing hands after completing all experiments. In addition, we will not take any products out of the laboratory. Any activity that may violate the rules and biosafety regulations is prohibited. After the experiment, we will strictly disinfect the experimental site to avoid any bacteria escaping from the laboratory.

    The strains used in our project, Escherichia coli (MG1655, DH5 α and BL21), are all classified as non-pathogenic class 1 organisms, which means that there is no impact on human beings and no impact on the environment. In addition, to increase the safety of the experiment, we integrated the toxic protein ccdB gene regulated by arabinose-induced promoter into the bacterial genome, so as to achieve bacterial suicide death by adding arabinose when necessary.

    And the parts and products used in this design are non-toxic or low-toxic products, and the combination of parts will not produce harmful substances to human body or environment. And the combination of parts will not produce substances harmful to human body or environment.