Using bioinformatics, we screened five RBS sequences from E. coli MG1655 by software. We used yellow fluorescent protein YFP as reporter gene, and measured the fluorescence intensity in the bacterial solution by microplate reader to reflect the expression of YFP, thus verifying the corresponding RBS intensity. The following are the experimental results.

The results showed that the strain using RBS3 expressed the most yellow fluorescent protein, which indicated that the intensity of RBS3 was the highest among the five RBS. We hope that the RBS information provided by our team will help future igem teams in choosing RBS.