Overview of all experiments performed to optimize PHA production using M. extorquens
Below you can find the lab notes of all the performed experiments in chronological order. They are grouped per research line:
1. Promoter characterization
2. Overexpression of phaC, groEL and groES
3. Carotenoid biosynthesis pathway knockout
4. Inducible autolysis system
5. PHA extraction and M. extorquens cultivation
6. PHA identification
The description and results of each research line can be found on the Engineering page and Results page , respectively. The detailed protocols can be found on the Protocols page . The notebook was written using Benchling.