During the virtual meet-up with the members of LZU-China,both of our two teams introduced our projects respectively and asked questions about each team regarding wet lab and modeling technique, and we realized that iGEM LZU team has a similar project with us. Thus, we set up a tight relationship with LZU team in not only wet lab part but also human practice aspect . Based each of our module design on safety control, we exchanged our plasmids Pasr-mcherry-pSB1C3.gb with their plasmids pSB1A3-hok,sok because we want to get more relevant information and data to make our whole project more complete. In return,we use our own materials and samples to test for them in our lab, because we don’t have the strain of EcN that LZU-China use in our own lab, so after discussing with LZU-China we decide to use E. coli DH5-alpha directly to test if dh5- alpha strain.

Figure 3.3.44. The Plasmid shared with LZU-China.

The plasmid design map LZU-China shared to UM-Macau on the module of safety control module.

Meanwhile, UM-Macau and LZU-China exchanged educational materials such as powerpoints and syllabus, and based on the conditions of both teams, we shared resources and used the exchanged materials to conduct educational activities with each other's communities for different school-age audiences. This collaboration has successfully reached primary, high school and undergraduate populations in Macau and Mainland China, and by leveraging LZU-China's local resources, the wisdom of the UM-Macau project has been able to successfully reach out across thousands of miles to teach synthetic biology to children in remote areas.

By helping each other on the wet lab, project design and education level to test our hypothesis and spread our impact ,both of us gain a lot in our collaboration.

As an important industry-academia-research demonstration base established by the University of Macau in Zhuhai, Guangdong Province, ZUMRI responded to our call to organize the 1st iGEM Guangdong, Hong Kong and Macao Greater Bay Area Synthetic Biology Industry-Academia-Research Forum, and provided us with a lot of manpower and material support for the event. ZUMRI provided us with more opportunities to visit enterprises, so that UM-Macau could get a lot of effective feedback on our project, which helped us to establish a more comprehensive perspective on product development from the production and practical point of view. This helped us to build a more comprehensive view of product development from a production perspective. At the same time, through UM-Macau's synthetic biology oriented communication, the downstream companies with which ZUMRI has a close working relationship have been inspired by us, and our wisdom and efforts have inspired ZUMRI to develop new R&D directions in synthetic biology in areas such as cancer diagnostics, stem cell research, and so on. Through UM-Macau, synthetic biology is expected to form a new growth pole in the Guangdong-Hong Kong-Macau Greater Bay Area.

Figure 3.3.45 The logo of ZUMRI

Zhuhai Hengqin Imstem Biotechnology Co., Ltd is an innovative biotechnology company dedicated to the independent research and development of stem cell-based medicines, focusing on clinical treatments for autoimmune diseases, degenerative diseases, genetic diseases and other clinical treatments as well as in the field of medical anti-aging, addressing unmet medical needs and meeting higher therapeutic goals.

Figure 3.3.46 The logo of Zhuhai Henqin Imstem Biotechology Co., Ltd

Established in 2016, Nanjing helptherapeutics Co., Ltd. is the first company in China to develop a new generation of universal cell therapy products using induced pluripotent stem cells and mesenchymal stem cells to treat clinically intractable degenerative diseases, leading the way in regenerative medicine to meet the urgent needs of the clinic and to reshape the human body's health.

Figure 3.3.47 The logo of Nanjing helptherapeutics Co., Ltd.

Sanmedgene Co., Ltd. is a high-tech service-oriented enterprise specializing in medical testing services, with a medical testing laboratory licensed to practice as a medical institution. Shengmei Genetics is committed to the development and application of precision medical testing for tumors, based on liquid biopsy, identification of benign and malignant lung nodules, and tumor immunotherapy testing, etc.

Figure 3.3.48 The logo of Sanmedgene Co., Ltd.

SnapGene is a molecular biology software that helps millions of researchers worldwide to design plasmids easily through visualization and simulation. They gently offered us a free license to use SnapGene in our project.

Figure 3.3.49 The logo of Snapgene.

IDT (Integrated DNA Technologies)’s primary business is the manufacturing of custom DNA and RNA oligonucleotides for research application. This year IDT works as our partner to offer us 20k bp DNA fragment for free to use in our plasmid design—The insert gene related to endopeptidase and NO sensor we use in our experiment are provided by IDT to support our project.

Figure 3.3.50 The logo of Integrated DNA Technologies.