Safety
On this page, we want to make it clear to the public that our project is completely safe, and we take a number of ways to ensure safety
Our project is to use two proteins, HMGB1 and PslG, to disrupt the human intestinal pathogen biofilm. These two proteins are widely and naturally expressed in mammalian cells and bacteria, respectively. We will express them in the Nissle 1917 strain of E. coli and test the activity and decomposition effect of each of them on the biofilm. Among them we use Pseudomonas aeruginosa as a source of biofilm. Finally, we will grow human intestinal epithelium cells to mimic the intestinal environment and test their pungency. Prior to all experiments, all participants working in our laboratories were trained in safety and basic laboratory procedures to prevent leakage and misconduct. The laboratory where we work is equipped to process E. coli Nissle 1917 and Pseudomonas aeruginosa and prevent leaks with protective measures. For example, we will disinfect all contaminated items before disposal. All single-use waste is put into biomedical garbage bags.
Throughout the experiment, we were supervised by medical professionals to not only ensure safety but also to execute good practices.
With any experiment that is to be deployed in the laboratory, there is a risk of release of the used organism. As our project involves culture and storage of microorganisms, we took extra precautions. To ensure that the experiment had effective biocontainment to minimize risks, molecular cloning and protein purification refer to existing standard protocols. The activity detection is also refers to similar studies.
E. coli Nissle 1917，and human intestinal epithelium cells are used in our experiments. These cells do not pose any risk to us and our colleagues in the laboratory, and the bacteria and cells we use are harmless to the environment and to humans. The use and management of bacteria in the laboratory is standardized and there is no risk of leakage. Pseudomonas aeruginosa is conditionally pathogenic bacteria, and can be widely found in nature, human skin, intestinal and respiratory tract. If leaked, could cause a light infect on people with weakened immunity.
The laboratory where we work has been kept bright, neat and good environment, which provides a guarantee for the safety of the experiment.
Our laboratory also has physical containment level 2 laboratory, which can ensure the safety of the experimenter and the environment to the greatest extent.
In addition, the students who carried out the experiment have obtained relevant proofs before doing the experiment to ensure the compliance of the experiment and ensure the safety of the experiment
