IGEM is based on a combination of viral RNA and viral protein to study HIV-1 RRE RNA and HIV-1 Rev protein. In vitro transcription of RNA and overexpression of Rev proteins can provide a reliable molecular basis and mechanism of action for the development of viral RNA vaccines, nucleic acid integrase inhibitors, and site-targeted drugs. We validated and characterized the interaction after in vitro transcription of HIV-1 RNA and overexpression of Rev protein.

HIV-1 RRE is a partial sequence of the HIV-1 isolate ARV-2/SF2 strain gene. Its strain belongs to the main epidemic group subgroup and is mainly responsible for the global HIV epidemic. HIV-1 RRE RNA is a long non-coding RNA, and there is insufficient evidence to study the mechanism of action of full-length RRE RNA and full-length viral Rev protein together, indicating that further research is needed.

Our team constructed the HIV-1 RRE full-length gene sequence to prepare RRE RNA, and synthesized a plasmid from Qingke Biological Company. The length of the plasmid gene is 2484bp, of which the HIV-1 RRE gene sequence is 358bp.

When using HIV-1 RRE DNA amplification, HIV-1 RRE DNA templates are generated from BBa_K4996000, BBa_K4996002, and BBa_K4996003, and then transcribed to achieve high-quality RRE RNA.

HIV-1 retrovirus replication and translocation of endogenous retroelements utilize a unique post-transcriptional regulatory mechanism of partially spliced or unspliced mRNA export into the cytoplasm [1]. HIV-1 Rev is a partial sequence of the HIV-1 isolate ARV-2/SF2 strain gene and is primarily responsible for the global HIV epidemic. However, the research on its molecular mechanism is still insufficient, and its research is of great significance.

We also constructed the gene sequence of the HIV-1 Rev protein at the same time and ordered an overexpression vector through a biogene technology company. The plasmid gene HIV-1 Rev is 5662 bp in order to prepare the HIV-1 Rev protein. The length of the HIV-1 Rev gene is is 429bp.

Through plasmid amplification, colony culture, overexpression, isolation and purification of HIV-1 Rev from BBa_K49960001, a high-quality overexpressed protein can be obtained to interact with HIV-1 RRE RNA to provide effective proteins and develop action sites. Provide molecular mechanisms for target drugs.

HIV-1 RRE is a partial sequence of the HIV-1 isolate ARV-2/SF2 strain gene. Its strain belongs to the main epidemic group subgroup and is mainly responsible for the global HIV epidemic. HIV-1 RRE RNA is a long non-coding RNA, and there is insufficient evidence to study the mechanism of action of full-length RRE RNA and full-length viral Rev protein together, indicating that further research is needed.

Our team constructed BBa_K4996000 to successfully prepare the RRE DNA template and synthesized the forward primer, 30 bp, from Qingke Biotech.

The target gene HIV-1 RRE DNA template is generated from BBa_K4996000, BBa_K4996002 and BBa_K4996003, and then transcribed to achieve high-quality RRE RNA.

HIV-1 RRE is a partial sequence of the HIV-1 isolate ARV-2/SF2 strain gene. Its strain belongs to the main epidemic group subgroup and is mainly responsible for the global HIV epidemic. HIV-1 RRE RNA is a long non-coding RNA, and there is insufficient evidence to study the mechanism of action of full-length RRE RNA and full-length viral Rev protein together, indicating that further research is needed.

Our team constructed BBa_K4996000 to successfully prepare the RRE DNA template and synthesized the reverse primer, 28 bp, from Qingke Biotech.

The target gene HIV-1 RRE DNA template is generated from BBa_K4996000, BBa_K4996002 and BBa_K4996003, and then transcribed to achieve high-quality RRE RNA.