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Our Contributions

Peking iGEM 2023 has designed and characterized a large number of basic parts and composite parts, and the relevant parts have been uploaded to http://parts.igem.org.


Hypoxia-inducible promoters


PartsTypeRegistry Part ID
pPepT promoter (wild-type variant)basicBBa_K4713111
pPepT promoter (TATA-box mutated variant)basicBBa_K4713112
pPepT promoter (FNR-box mutated variant)basicBBa_K4713113
pPepT promoter (both TATA- and FNR-box mutated variant)basicBBa_K4713114
FF+20 promoterbasicBBa_K4713115
EcN endogenous pPepT promoterbasicBBa_K4713116
pPepT promoter (wild-type variant) with RBS (BBa_B0034)compositeBBa_K4713117
pPepT promoter (wild-type variant) with RBS (BBa_J61101)compositeBBa_K4713118
pPepT promoter (TATA-box mutated variant) with RBS (BBa_B0034)compositeBBa_K4713119
pPepT promoter (TATA-box mutated variant) with RBS (BBa_J61101)compositeBBa_K4713120
pPepT promoter (FNR-box mutated variant) with RBS (BBa_B0034)compositeBBa_K4713121
pPepT promoter (FNR-box mutated variant) with RBS (BBa_J61101)compositeBBa_K4713122
pPepT promoter (both TATA- and FNR-box mutated variant) with RBS (BBa_B0034)compositeBBa_K4713123
pPepT promoter (both TATA- and FNR-box mutated variant) with RBS(BBa_J61101)compositeBBa_K4713124
FF+20 promoter with RBS (BBa_B0034)compositeBBa_K4713125

Acoustic response genes


PartsTypeRegistry Part ID
T7 promoter + lac operator + ARGcompositeBBa_K4713100
T5 promoter (with lac operator embedded)basicBBa_K4713106
T5 promoter (with lac operator embedded) + ARGcompositeBBa_K4713105

Hybrid promoters sensing hypoxia and high lactate


PartsTypeRegistry Part ID
lldRO1-PepT-lldRO2 (NS-WA)basicBBa_K4713001
lldRO1-PepT-lldRO2 (NS-WT)basicBBa_K4713002
lldRO1-PepT-lldRO2 (S1-WA)basicBBa_K4713003
lldRO1-PepT-lldRO2 (S1-WT)basicBBa_K4713004
lldRO1-PepT-lldRO2 (S1-FT)basicBBa_K4713005
lldRO1-PepT-lldRO2 (S3-WA)basicBBa_K4713006
lldRO1-PepT-lldRO2 (S3-WT)basicBBa_K4713007
lldRO1-PepT-lldRO2 (S3-FA)basicBBa_K4713008
lldRO1-PepT-lldRO2 (S3-FT)basicBBa_K4713009
lldRO1-PepT-lldRO2 (L1-WA)basicBBa_K4713010
lldRO1-PepT-lldRO2 (L1-WT)basicBBa_K4713011
lldRO1-PepT-lldRO2 (L1-FA)basicBBa_K4713012
lldRO1-PepT-lldRO2 (L2-WA)basicBBa_K4713013
lldRO1-PepT-lldRO2 (L2-WT)basicBBa_K4713014

Inducible lldR overexpression part


PartsTypeRegistry Part ID
lldRbasicBBa_K4713101
T5 promoter (with lac operator embedded) + lldRcompositeBBa_K4713109

We extend our gratitude to everyone who has contributed to this project and welcome further collaboration and discussion with all interested parties.