Peking University made its iGEM debut in 2007 and has since undertaken several innovative projects over the past 16 years. Between 2007 and 2023, iGEM served as an academic platform for Peking, paralleling the university's witness to iGEM's own evolution. This year, the Peking 2023 team returns to the in-person venue with their new project, 'Cancer Assassin'. We are confident that our comprehensive efforts will meet the criteria for the following medals. To clearly display our work, we make this page as the navigational assistance.

Bronze Medal

1. Deliverables

· Wiki

· Project Promotion Video

· Presentation Video

· Judging Form

· Judging Session

2. Attributions

· Please visit our Attributions Page in the Team section for our attribution form.

3. Description

· Please visit our Description Page in the Project section for our project description.

4. Contribution

· We’ve made comprehensive contributions to iGEM. In brief, we designed and characterized new parts and introduced new models and hardware in promoter characterization. Please visit our wiki for further information.

Silver Medal

1. Engineering Success

· We’ve successfully constructed and characterized several genetic circuits. Please visit our Results Page and Engineering Page for more information.

2. Human Pracices

· We’ve made comprehensive human practices. Please visit our Human Practices Page in the HP and Education section for more information.

Gold Medal

Best New Basic Part

We successfully designed, constructed and characterized 14 hypoxia-lactate-induced hybrid promoters (BBa_K4713001—BBa_K4713014). Among them, we selected the promoter with the best performance (BBa_K4713008), which has great potential in tumour-targeting bacterial therapies. Compared to conventional AND-gate, a hybrid promoter requires no expression of assistant proteins, and therefore consumes less energy, which can enable the engineered strain to carry more modules in future studies. Visit our Parts Pages(related information is available on the Contribution Page in the wiki), Results Page and Engineering Page

Best Model

To support our project, we developed models to estimate the performance of our conceptual modules. The main goal of the Hybrid Promoter Predicting Model is to filter out a promoter sequence with the best distinction under conditions with or without environmental oxygen and under different lactate concentrations. We also developed the MC Model to simulate bacterial distribution post-intravenous injection. After adjustment, these two models successfully work as the supporter of the wet lab. Please visit our Model Page in the Dry Lab section for further information.

Best Education

Leading a project centered around cancer, a life-threatening disease, necessitates profound discussions on life and death.. Observing the deficiency of life-and-death education in China, we launched a project to promote this type of education. In addition, we launched another program to popularize synthetic biology. Spanning 13 provinces, including Hong Kong and Macau, this initiative comprised both lectures and laboratory sessions. Please visit our Education Page in the HP and Education section for further information.