Organizing campus promotions for iGEM 2023.

Holding a biology contest to select team members for NUDT-CHINA 2023.

Establishment of team NUDT-CHINA 2023. Team member chose their interesting role to play according to their interests, including Wet Lab, Human Practice, Art&Design.

Conducting a literature review on industries associated with obesity.

Conducting a questionnaire survey among over 2,000 participants.

Brainstorming for 2023 team project.

Learning the molecular biology and basic experimental techniques.

Laboratory safety training.

Determining the initial direction of the 2023 iGEM project, considering to choose UCP1 as our fundamental weight-losing protein to deliver. However, the specific method to deliver remained undecided.

Determining the direction of using PVCs as delivery tool. Til here, project ARROW was born.

Conducting preliminary Proof of Concept design and plasmid design.

Conducting an interview with Professor Zhang. Our dedication to the project became stronger.

Collection and study of relevant literature regarding the application of our research project.

Development of the 1st Generation ARROW Head protein.

Preliminary Safety Form and Project Description.

Start modeling for UCP1 functionality.

Offline meeting of the Wet Lab group for literature reading, reporting the progress of the project and arranging the follow-up tasks.

Team uniform & Project logo & Team 10th Anniversary Logo design.

Online meetings of the experimental group for literature reading and plasmid design.

Confirming PVC specificity with PVCs that tail fibers were modified with DARPin and gain positive results.

Conducting AlphaFold2 Multimer V3-based structure prediction for reprogrammed PVC.

Start constructing 25K PVC plasmid with normal molecular strategy.

Conducting AlphaFold2-based structure prediction for ARROW Head structure.

Interview with drug quality monitoring expert Xiao. Inspired with his advices on ARROW further implementation.

Coming up with the 2nd Generation ARROW Head protein, changing the UCP1 to the C-terminus of the fusion protein.

Production of the Promotion Video.

Creation of team giveaways and mascots.

Offline meeting of the wet lab group for further design and engineering.

Commencement of production for the “Gene Editing” Science Popularization Picture Book.

Team Roster Confirmed.

Attending National Science Popularization Day Series Events.

Purifying PVCs (loaded with 2nd Generation ARROW Head protein) with different E.coli culture condition.

Interview with master Zhou. Inspired with effective Golden Gate assembly method.

Conduct 2nd Generation ARROW Head delivery experiment to Hela Cells.

Interview with expert from drug company Xiao. Informing us with detailed possible further application of our project.

Team family photo.

Interview with synthetic biology experts Zhou about 3rd ARROW Head Constuction.

WIKI Construction.

Production of Presentation Video.

Participation of Grand Jamboree.