In Vitro PVC Delivery Experiments
Protocol for Cell Culture and Cubculture
Protocol for Cell Transfection and Stimulation
Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis
Agarose Gel Electrophoresis
Double Enzyme DNA Digestion& Agarose Gel Electrophoresis
Electroporation
Gel Purification
Infusion
Preparation of Electrocompetent Bacterial Cells
Overlap PCR & Agarose Gel Electrophoresis
PCR & Agarose Gel Electrophoresis – Colony PCR
PCR & Agarose Gel Electrophoresis
Plasmid Conservation
Plasmid Extraction
Protocol for LB Medium Preparation
PVC Purification
Transformation