| Works only in absence of glucose and presence of oxygen, limiting its broader applications |
Works in hypoxic and in the presence of glucose conditions, applicable for cancer therapy |
| Expressed in high copies which poses a metabolic stress on a host cell |
Expressed in pET9a, a low-copy plasmid, removing metabolic burden from the cell |
| Repressible in anoxia by ArcA |
The binding of LldR protein O1 and O2 leads to the formation of a DNA loop that blocks transcription. Once L-lactate is present, LldR binds it, undergoes a conformational change, and RNA polymerase can “sit” on promoter |
| Figure 2 |
Figure 3 |
