1. Project safety

     Using synthetic biology methods, the team aims to prepare hydrogen-rich water for plant irrigation. In the production process, we produce target proteins through engineered bacteria, which are subsequently cell-disrupted to obtain the desired enzymes. Subsequently, we used methanol as a substrate to produce hydrogen-rich water through the catalysis of four enzymes. Eventually, we use hydrogen-rich water for plant irrigation. The entire process ensures that microorganisms are not released into the environment, while team members strictly follow appropriate safety measures to ensure the safety of operators.

Figure 1: Experimental procedure

Safety is of significant importance in the practical basis for successful competition. At all stages of the competition, our team is extremely sensitive to security issues. First, team members undergo comprehensive safety training before stepping into the lab to ensure they are highly aware of potential hazards. In addition, safety considerations were carefully considered during the protocol construction phase to ensure that the experimental design minimizes potential risks. During the operation of the experiment, the team strictly adhered to safety guidelines to minimize the risk of accidents. Finally, comprehensive security measures are implemented within the laboratory to ensure maximum safety for team members and the environment

The team members strictly follow the safety regulations of the IGEM competition, adhere to the safety guidelines in the laboratory, and comply with national biosafety regulations. As a result, the project adheres to high safety standards throughout its process, ensuring that its safety is fully guaranteed.

To verify that the protein solution produced by Gmos does not contain any Gmos, we coated the prepared mixture of five protein solutions on a plate and coated the plate with receptive cells BL21(DE3) as a positive control. Subsequently, the plates were incubated overnight in a 37℃ incubator, and the results were observed (as shown in Figure 1 below). The results showed that no microbial growth was observed on the plate coated with protein solution, but on the plate coated with receptive cell BL21(DE3). This indicated that the protein purified by Ni-NTK did not contain any genetically modified organisms

Figure 2: Safety verification test results. (A) protein solution coating results; (B) BL21(DE3) receptive cell coating results