Experiment name and purpose
In recent years, a large number of studies have shown that hydrogen-rich water has a positive effect on plant growth. The team hopes to use synthetic biology to produce hydrogen-rich water for crop watering, replacing part of the fertilizer and reducing the amount of fertilizer, thereby protecting the soil and ecological environment.
Experimental design and methods
The team's experiments are divided into molecular cloning experiment and botanical experiment. Methanol dehydrogenase, formaldehyde dehydrogenase, formate dehydrogenase, hydrogenase and protein scaffold are obtained through molecular cloning experiment for the production of hydrogen-rich water. And the hydrogen-rich water was used in botany experiment to verify the botanical effect of hydrogen-rich water.
Experimental results
The team successfully obtained 4 enzymes and successfully produced hydrogen-rich water in the liquid phase system through a cascade reaction of 4 enzymes
Data analysis
The team used a protein scaffold to combine the four enzymes to form a multi-enzyme complex to improve the hydrogen production efficiency of the entire system. Experimental verification showed that the hydrogen production efficiency of the hydrogen production system using the protein scaffold was 1.58 times higher than that of the free enzyme system and 24.8 times higher than that of the traditional biooptical hydrogen production.
Conclusion of the experiment
The preparation of hydrogen-rich water catalyzed by whole enzyme method using methanol as substrate provides a new idea for soil protection and fertilizer reduction, which is expected to be put into large-scale agricultural production in the future.