Our favorite composite part is BBa_k4927058.
BBa_k4927058 is our protein scaffold based on E. coli IM and CE, Composed of BBa_J64997, BBa_K4927048, BBa_K4927008, BBa_K4927001, BBa_K4927009 BBa_K4927002, BBa_K4927010, BBa_K4927003, BBa_K4927011 And BBa_K4927043. In these parts, IM2, IM7, IM8 and IM9 will combine with corresponding enzymes labeled CL2, CL7, CL8 and CL9 to form artificial multi-enzyme complexes (FIG. 1), forming substrate channel effect and improving the reaction efficiency of the whole system. For more information, please click BBa_k4927058.
Figure 1: Schematic diagram of the genetic circuit of the protein scaffold