As a leading institution in synthetic biology, iGEM acts as a testing ground for new ideas and approaches in synthetic biology. An important challenge in the field of synthetic biology is to ensure that reliable and repeatable measurements are available in different laboratories, a core element of engineering. Often, for example, it is not possible to compare fluorescence data from different LABS because they are reported in different units, or because different study groups process the data in different ways. This complicates adjusting the structure of engineered organisms, validating existing measurements, sharing data between laboratories and even interpreting experimental controls. In recent years, the iGEM team has addressed this challenge through international Interlab measurement research. In previous Interlab studies, the focus was on developing robust green fluorescent protein and cell density (OD600) measurement procedures to quantify bacterial fluorescence and cell count through the use of independent calibrators. This helped ensure consistent and comparable results in synthetic biology studies.

     In 2023, the HUBU-SKY-China team contributed by participating in the "Experiment One" InterLab study.In Experiment I, our team measured the fluorescence of six devices encoding a single fluorescent protein (blue, green, or red) or two fluorescent proteins encoded in two transcription units, and we calibrated the fluorescence of these devices according to three calibration dyes and the light density of the culture according to a cell density calibrator. The data provided by our team are helping to advance research and development in the field of synthetic biology.