We have proof of concept demonstration in genetic engineering and chemical modification of bacteria, measurement, mathematic modeling, home-built portable device and software.

We have conducted the construction of recombinant plasmid containing the designed parts. We have completed the extraction of full-length RNA from cells, reverse transcriptase-polymerase chain reaction (RT-PCR), generation of cDNA for gene cloning, construction of recombinant plasmid and transformation in E. coli. Nissle 1917. We have performed thiolation of bacteria surfaces and the linkage with manganese dioxide nano-immunomodulators decorated with Au nanoparticles. We have established a 3D spheroid tumor model that can mimic the hypoxic and acidic microenvironment of tumors. Further full functions will be characterized in 2024. The implementation of engineered bacteria may take very long time more than 10 years. You can visit our Project page to see more details.

A LC-MS mass spectrometric technique and bioinformatic analysis method have been developed to monitor the changes in proteomic and metabolomic changes of bacteria in response to lactic acid. Please visit our Measurement and Results page for more information.

A mathematic model was established for proteomic and metabolomic analysis as well as monitoring adaptative evolution of bacteria in response to lactic acid. Our established mathematic model has been demonstrated with E coli Nissle 1917 that can live in lactic acid. A detailed report can be found in our Model page.

A home-built portable device and software have been established for the fundamental testing. In this year, the theoretical principle has been investigated and the fundamental hardware has been completed. Associated software is provided on the our iGEM wiki for free download. About three years later, the portable device and software may be completed and made as a product available to those people who need it. For detailed information, please visit our Hardware pages.