We have proposed implementation in educational program, social services, measurement, mathematic modeling, home-built portable device and software, and genetic engineering and chemical modification of bacteria.

In 2023, we have already provided a series of educational lectures, consulting seminar, brainstorm, offline meeting, field trip to conference, co-authored handbook, science popularization lecture in the university, communities of cities and rural counties,. We have published 20 articles (10 in 2023) in the WeChat account that has already been open to the society from March of 2022 (see Human Practice page for more information).

The LC-MS mass spectrometric technique and bioinformatic analysis method for proteomic and metabolomic analysis (see Measurement and Results page for more information) may be published in the near future. We can share the detailed experimental protocols with other people.

We have completed one mathematic model. It has been demonstrated in proteomic and metabolomic changes of bacteria living in lactic acid. A detailed report can be found in our Model page.

In 2023, the theoretical principle and hardware have been established. This home-built device was set up for the fundamental testing. Associated software is available on our IGEM wiki for free download. About three years later, the portable device and software may be completed and made as a product available to those people who need it. For detailed information, please visit our Hardware page.

In 2023, we have completed the extraction of full-length RNA from cells, reverse transcriptase-polymerase chain reaction (RT-PCR), generation of cDNA for gene cloning, construction of recombinant plasmid and transformation in E. coli. Nissle 1917. We have performed thiolation of bacteria surfaces and the linkage with manganese dioxide nano-immunomodulators decorated with Au nanoparticles. We have established a 3D spheroid tumor model that can mimic the hypoxic and acidic microenvironment of tumors. Further full functions will be characterized in 2024. The implementation of engineered bacteria may take very long time more than 10 years. You can visit our Project page to see more details.