Medal
Gold Medal
Best Model
We used models and computer simulations to analyze the specificity, stability and efficiency of
LwaCas13a/crRNA/miR-21-5p ternary complex before and in conjunction with the experiments in the wetlab,
which
helped us inform system design, simulate expected behavior before, and verify the results of experiments.
Click
the button below for details about Model.
Model
Best Integrated Human Practices
We established a two-way dialogue with the world, influenced each other with stakeholders. To ensure that
BreFast is responsible and beneficial to the world, we spread values and introduced BreFast to general
public,
and constantly collected and integrated the suggestions from different communities throughout our project,
ultimately realizing “close the loop”. Click the button below for details about Integrated Human Practices.
Integrated Human
Practices
Best Education
We strove to include more people in shaping synthetic biology through innovative school and outreach
activities. Public values were conveyed to us through the street interviews and internet, and synthetic
biology
has been publicized to full coverage of education (primary school, middle school, high school, university,
and
village school) through our efforts. Click the button below for details about Education.
Education
Silver Medal
Engineering Success
We achieved engineering success by two engineering design cycles. Through engineering, we finally optimized
the
simulation and validation pipeline of CRISPR/Cas13a system, and succeeded in Cloning and Expression of
crRNA.
Click the button below for details about Engineering.
Engineering
Success
Human Practices
We constantly interacted with the world and gathered feedback to guide our project’s purpose, design, and
lab
experiments. Click the button below for details about Human Practices.
Human
Practices
Bronze Medal
Competition Deliverables
We have and will fully complete the required deliverables.
- Wiki (finished)
- Promotion Video (finished)
- Presentation Video (finished)
- Judging Form (finished)
- Judging Session (will fully complete it)
Project Attributions
We have clearly attributed all the work completed by the team members and external help in Attributions
Page
using Attribution Form. Click the button below for details about Attributions.
Attributions
Project Description
Early detection can reduce the mortality rate of breast cancer effectively. Therefore, we focused on
creating
a
fast and convenient breast cancer screening kit, BreFast. Click the button below for details about
Description.
Description
Contribution
In terms of model, We have used a range of bioinformatic methods to enhance the accurate identification of
target miRNAs and introduced a feature importance method based on random forests into bioinformatics
analysis,
which can be used for similar iGEM projects. In terms of experiment, the crRNA production method can be used
by
other iGEM teams. Click the button below for details about Contribution.
Contribution
