March

On March 2nd, our team was officially established. Although we didn't have a formal team name at this time, our initial team was still actively brainstorming all night for our project.In the initial meeting on March 2, we also had a preliminary division of labor.On March 4, under active discussion, we produced our initial logo and the first official account tweet. Although we are not good at painting in biology, everyone's enthusiasm is enough to support us to move forward.

Week 2 (March 5 to 11)

On March 6th, we held our first large formal meeting with other teams and instructors from inside and outside the school, and everyone was in high spirits! This is an expected debriefing on the work in the coming months. We had the opportunity to listen to teachers from Chen Weizhao, Yang Jian and Zhang Lijun talk about their team's experience in participating in iGEM over the years, and showed us all the rigorous and interesting activities of the team. Teacher Yang Shihui provided us with a global perspective on what it means to become iGEMmer. We also reported the products of our own brainstorming, where the teachers evaluated and guided our ideas and answered our questions. It was a very interesting event and all of us were happy to be part of the iGEM world.

Week 3 (March 12 to 18)

We held several group discussions this week, brainstorming for our project and analyzing and interpreting the competition information on the official website.On March 18th, the second large-scale exchange and guidance meeting was held. After listening to the competition experience of several instructors, we were greatly touched. After discussion and research, we sorted out the general framework of the competition and had a deeper understanding of the specific project arrangement.

Week 4 (March 19 to 25)

We began to discuss and analyze the excellent teams and projects of previous years, hoping to learn from their work that are missing in the initial plan.Our project is taking shape. From the fallen leaves on the campus, we think of these plant "waste" piled up in the land, to transform them. Although the idea was questioned by the feasibility of the PI, it overall provided ideas for our project.

Week 5 (March 26 to April 1)

This week, we organized the knowledge sharing about laboratory safety in the group. The PI shared the important safety knowledge in the laboratory with all the members of our team. Each of us has recorded these knowledge carefully, and studied the "Biosafety Law of the People's Republic of China" and "Hubei University Laboratory Safety Guidelines".

April

Week 6 (April 2 to 8)

This week we set out the project theme and focus on the synthetic biology part of the project and how to use molecular biology strategies to implement these ideas.We propose the idea of anchoring multiple cellulase constituent fibrosomes to the cell surface.

Week 7 (April 9 to 15)

This week, we officially entered the lab for experiments.We mainly completed the surface display of two cellulases in S. cerevisiae.The third cellulase did not exhibit enzymatic activity on the surface display system.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun

Week 8 (April 16 to 22)

We modified the inducible expression medium after the literature review and repeated the experiment again.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun.We also proposed to produce popular science articles to publish on our team account to promote our team project while spreading biological knowledge.On April 22, he published its first popular science article, "Have you ever heard of synthetic biology?".
Participant:Wang Xinlingjun, Luan Ziyi, Chen Yanjing

Week 9 (April 23 to 29)

Last week's repeat experiment continued due to the low recovery of gene fragments.This week to complete the routine transformation of E. coli.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun We published the second popular science article "Laboratory safety Code" after organizing the knowledge of the last safety sharing meeting.
Participant:Xiao Yuhe, Luan Ziyi, Chen Yanjing

Week 10 (April 30 to May 6)

After validation of colony PCR, plasmids were extracted for linearization, enzymatically linked, and electrotransformed.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun We published this week's popular science article, "Animal rumination". Participant:Chen Kexin, Luan Ziyi, Chen Yanjing

May

Week 11 (May 7 to 13)

Given the low concentration of the recovered fragments after linearization, the last week's experimental work was repeated this week.The electrotransformed work was completed this week. Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun We published this week's popular science article, "Understanding cellulase".
Participant:Tian Chao, Luan Ziyi, Chen Yanjing
A new member of our team, Liu Xiongxiao, who is responsible for the design of art resources. He designed a whole new logo for our team.And he also provided support for cover production for our popular science articles.

Week 12 (May 14 to 20)

After electrotransformation, colony PCR was performed to verify the target fragment, and the results were not ideal, with no band after repetition, and electroconversion was redone.The electroconversion process was successfully completed and verified by colony PCR.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun We published this week's popular science article, "History of molecular biology development".
Participant:Wang Xinyu, Luan Ziyi, Chen Yanjing

Week 13 (May 21 to 27)

The electrotransformed yeast were induced expression, timed sampled, and enzymatic assays.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun
We published this week's popular science article, "What is polysaccharides".
Participant:Wang Xinlingjun, Luan Ziyi, Chen Yanjing, Liu Xiongxiao

Week 14 (May 28 to June 3)

The results of enzyme measurement are not good, find literature and analyze the reasons for failure.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun
We discussed proposing to introduce our project in the form of animation.Chen Kexin, Wang Xinling Jun proposed script; Liu Xiong Xiao made art resources; Wang Xinling Jun made animation; Wang Xinling Jun, Chen Kexin, Xiao Yuhe, He Yinqi dubbing; we co-production.
Participant:Wang Xinlingjun, Chen Kexin, Xiao Yuhe, He Yinqi, Liu Xiongxiao

June

Week 15 (June 4 to 10)

We modified the promoter for galactose-induced expression according to the summarized reasons for failure, while modifying the corresponding experimental protocol. Start back from scratch and complete the routine transformation this week.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun
We published this week's article, "Straw single-cell protein feed".We hope to show our project to everybody through this article.
Participant:Xiao Yuhe, Wang Xinlingjun, Luan Ziyi, Chen Yanjing

Week 16 (June 11 to 17)

This week, we discussed that the existing experimental scheme is too difficult to continue. After reviewing a large amount of literature and consulting the PI, we proposed to change the plan. The original protocol was changed to use gene fusion technology to fuse three cellulase genes for expression in yeast.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun Wang Xinlingjun contacted and made the team's brochures, team uniforms and team flags. The drawings of the uniforms and flags were drawn by Liu Xiongxiao, and the brochure was drawn by Wang Xinlingjun.

Week 17 (June 18 to 24)

We discuss and propose that the content other than the cellulose degradation pathway can also be designed to enhance the straw degradation.We started to collect the xylan degradation protocol and tried to incorporate it into our experiments.After initially establishing the experimental protocol, we first performed primer design to amplify the target fragment and screened them for mutations using error-prone PCR.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun, Li Huanan
We officially started the questionnaire survey on the project this week. This week, we have compiled a set of questionnaires for retail investors and farms, respectively.
Participant:Zhang Tian, Luan Ziyi, Xiao Yuhe, Chen Yanjing, He Yinqi, Huang Zining
We published this week's popular science article,"Xylosic alcohol".
Participant:Chen Kexin, Wang Xinlingjun, Luan Ziyi

Week 18 (June 25 to July 1)

After processing the gene fragments of xylanase, considering the possibility of forming the inclusion, we also set up the experimental group adding lyolytic fragments for manipulation.
Participant:Wang Xinyu, Chen Kexin, Xiao Yuhe, Tian Chao, Wang Xinlingjun
We published this week's popular science article,"Cell surface display system".
Participant:Tian Chao, Wang Xinlingjun, Luan Ziyi

July

Week 19 (July 2 to 8)

This week, the enzyme connection and routine transformation work were performed.Furthermore, we also tried to optimize the utilization of xylose by yeast.
Participant:Wang Xinyu, Chen Kexin, Tian Chao, Wang Xinlingjun
We have started our volunteer teaching activities since this week. We introduced our project to the children, and explained the biological knowledge to them carefully.
Participant:Xiao Yuhe
Together with the other four teams from Hubei University, we hosted the Hubei Synthetic Biology Open Conference. Gather a number of teams to report and communicate, and set up stalls to publicize the science related to the project.
Participant:Wang Xinlingjun, Xiao Yuhe, Wang Xinyu
We published this week's popular science article,"Xylanase".
Participant:Wang Xinyu, Wang Xinlingjun, Luan Ziyi

Week 20 (July 9 to 15)

This week we electrotransferred the xylanase fraction, but it was not verified by colony PCR.After replacing the promoter of xylose isomerase, the utilization efficiency of xylose and glucose has been greatly improved.
Participant:Wang Xinyu, Chen Kexin, Wang Xinlingjun
We published this week's popular science article,"Know S. cerevisiae".
Participant:Wang Xinlingjun, Luan Ziyi

Week 21 (July 16 to 22)

After repeating the experiment and having failed.In the experiment, it was found very unlikely that the target protein was displayed exocytotically, so we chose to extract it and add it directly to our products.
Participant:Wang Xinyu, Chen Kexin, Tian Chao, Wang Xinlingjun
We published this week's popular science article,"Gene fusion expression".
Participant:Xiao Yuhe, Wang Xinlingjun, Luan Ziyi

Week 22 (July 23 to 29)

We spent this week trying to purify xylanases and measured enzyme viability.
Participant:Wang Xinyu, Chen Kexin, Tian Chao, Wang Xinlingjun
We published this week's popular science article,"Error-pronePCR".
Participant:Chen Kexin, Wang Xinlingjun, Luan Ziyi

Week 23 (July 30 to August 5)

We started building the fusion gene fragments this week.
Participant:Wang Xinyu, Chen Kexin, Tian Chao, Wang Xinlingjun
We published this week's popular science article,"Risk of sugar addiction".
Participant:Tian Chao, Wang Xinlingjun, Luan Ziyi

August

Week 24 (August 6 to 12)

We transferred the constructed fusion expression vector into the yeast used for surface display and found that the yeast was unable to grow on the defective plates.
Participant:Wang Xinyu, Chen Kexin,Xiao Yuhe, Tian Chao, Wang Xinlingjun
We published this week's popular science article,"Why do you like eating spicy food".
Participant:Wang Xinyu, Wang Xinlingjun, Luan Ziyi

Week 25 (August 13 to 19)

We propose to discard the surface display technique and directly use the fusion gene for intracellular expression. The yeast species were replaced and the electrotransfer experiment was repeated.
Participant:Wang Xinyu, Chen Kexin,Xiao Yuhe, Tian Chao, Wang Xinlingjun
Once again, we compiled a questionnaire for the public to collect public opinion.
Participant:Luan Ziyi, He Yinqi, Xiao Yuhe, Zhang Tian, Wang Xinlingjun
Xiao Yuhe helped us to contact the feed manufacturers in Yueyang area of Hunan Province, and we went to the field investigation on August 18.We also made a field visit to the rural breeding retail investors in Hubei province on the project.
Participant:Xiao Yuhe, Wang Xinlingjun, Huang Zining
We published this week's popular science article,"Ahypnosis".
Participant:Wang Xinlingjun, Luan Ziyi

Week 26 (August 20 to 26)

Made the yeast receptor of the new species, linearized the plasmid, reconstructed the vector, and electrotransformed it.
Participant:Wang Xinyu, Chen Kexin,Xiao Yuhe, Wang Xinlingjun
Compile online and offline practice research reports.
Participant:Zhang Tian, Xiao Yuhe, Huang Zining, He Yinqi, Luan Ziyi, Chen Yanjing
We published this week's popular science article,"About mosquitoes".
Participant:Xiao Yuhe, Wang Xinlingjun, Luan Ziyi

Week 27 (August 27 to September 2)

Electroconversion plates were infected with miscellaneous bacteria, reworked the receptor state, and raised the linearized carrier concentration.
Participant:Wang Xinyu, Chen Kexin,Xiao Yuhe, Wang Xinlingjun
We published this week's popular science article,"Sprouted potato".
Participant:Chen Kexin, Wang Xinlingjun, Luan Ziyi

September

Week 28 (September 3 to 9)

After electrotransformation, it was transferred to the screening plate and found a hydrolysis circle around the bacteria, which was verified by colony PCR. Participant:Wang Xinyu, Chen Kexin,Xiao Yuhe, Wang Xinlingjun We published this week's popular science article,"Sea fire". Participant:Tian Chao, Wang Xinlingjun, Luan Ziyi

Week 29 (September 10 to 16)

Pick the bacteria with larger hydrolyzized ones and culture them in a shake flask. The enzyme viability of the three cellulases and the total filter paper enzymes were determined.The enzyme viability of the three cellulases was successfully determined, but the filter paper enzyme activity was abnormal.
Participant:Wang Xinyu, Xiao Yuhe, Wang Xinlingjun
Our team held a charity fundraising event with HBUT-China,HUBU-HUBEI-HUINA,HUBU-HUNA, HUBU-SKY-China,HUBU-HUNA-WHUAN-CHINA,HUBU-CHNA, simultaneously at Hubei University and Hubei University of Technology from September 16 to September 27.
Participant: Xiao Yuhe, Wang Xinlingjun
On September 16, we also went to the Moon Bay community in Wuhan, Hubei province for fundraising activities and science popularization lectures.
Participant: Xiao Yuhe, Chen Kexin, Wang Xinlingjun
We published this week's popular science article,"Why do you have allergies?".
Participant:Wang Xinyu, Wang Xinlingjun, Luan Ziyi

Week 30 (September 17 to 23) Redo the electroconversion and remeasure the enzyme activity.The experimental task is thus fully completed.
Participant:Wang Xinyu, Xiao Yuhe, Wang Xinlingjun
On September 20th, Wuhan-Hubei-China and HUBU-China went to Wuhan University together and had an in-depth project exchange with WHU-China team. This exchange played a certain role in promoting the improvement of our project.
Participant:Chen Kexin, Luan Ziyi
We published this week's popular science article,"Why do you leave a scar?".
Participant:Wang Xinlingjun, Luan Ziyi

Week 31 (September 24 to 30)

This week,we wrote the wiki-related text and designed the web layout.
Participant:Tian Chao, He Yinqi, Xiao Yuhe, Huang Zining, Wang Xinlingjun Together with four other teams from Hubei University, we held an exchange and science popularization meeting on the life science competition.
Participant:Chen Kexin, Wang Xinlingjun
We published this week's popular science article,"Mango Little Knowledge".
Participant:Xiao Yuhe, Wang Xinlingjun, Luan Ziyi

October

Week 32 (October 1 to 7)

This week, we optimized the wiki page, and make presentation videos.
Participant:Tian Chao, Chen Kexin, He Yinqi, Xiao Yuhe, Huang Zining, Wang Xinlingjun
We published this week's popular science article.
Participant:Chen Kexin, Wang Xinlingjun, Luan Ziyi

Week 33 (October 8 to 12)

This week, we optimized the wiki page, and make presentation videos.
Participant:Tian Chao, Chen Kexin, He Yinqi, Xiao Yuhe, Huang Zining, Wang Xinlingjun