RECOVER: Revolutionizing CO2 Capture And Isobutanol Production By Engineered E.coli.


ABSTRACT:

The world confronts a critical climate crisis due to surging anthropogenic greenhouse gas emissions, mainly carbon dioxide (CO₂), predominantly originating from fossil fuels, accounting for nearly 90% of CO₂ emissions. Urgent action is imperative to safeguard the environment for future generations. Team REC CHENNAI is committed to combating climate change through Project RECOVER. The project targets CO₂ emissions by deploying an advanced hybrid carbon capture and biofuel production system. It involves creating an innovative exhaust gas recovery system, efficient CO₂ capture technology, and a biosystem utilizing engineered E.coli K12 for isobutanol production within a ship-compatible reactor. The engineered E.coli strain is designed to express four recombinant proteins: PRK and RuBisCO for CO₂ fixation, and KIVD and ADHA for isobutanol production. Through the seamless integration of cutting-edge hardware technology, we are establishing efficient, scalable systems to provide sustainable, technically sound solutions for addressing the climate crisis.

The Global Climate Crisis Unveiled


In the midst of our contemporary age, the climate crisis stands as an undeniable reckoning reshaping our world. No corner of the globe is immune from the devastating consequences of climate change. Rising temperatures are fueling environmental degradation, natural disasters, weather extremes, food and water insecurity, economic disruption, conflict, and terrorism.The Intergovernmental Panel on Climate Change (IPCC) has issued four significant reports on climate since its establishment, providing a solid scientific basis for the United Nations Framework Convention on Climate Change in 1992. This treaty commits nations to stabilize greenhouse gas levels in the atmosphere to prevent dangerous climate interference. In October 2018, IPCC stated that the world must limit global warming to 1.5 °C to achieve the goal of net-zero greenhouse gas (GHG) emissions by the mid-21st century.

Greenhouse Gases and Environmental Concerns


Greenhouse gases, including carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O), constitute a significant environmental concern. Among these gases, CO2 emerges as the predominant contributor to the greenhouse effect, with severe consequences for both human health and the habitable environment. Various methods for electricity production exist, each with varying degrees of impact on the surrounding ecosystem. Nonetheless, there are alternatives such as nuclear power plants, which do not emit CO2 during their operation. However, they are not without risk, and nuclear waste management poses a considerable challenge. Despite these alternatives, the utilization of fossil fuels, encompassing coal, gas, and oil, remains the most prevalent and widely practiced means of electricity production, significantly elevating the percentage of CO2 in the atmosphere. Renewable energy, including solar and hydroelectric power, offers hope in reducing emissions at the source. However, addressing fossil fuel emissions is still an urgent necessity due to their immediate and profound impact on our planet. These renewables have a strong negative correlation with CO2 production and support environmental sustainability. Addressing the dire consequences of climate change necessitates both international collaboration and active involvement from the research community.

Maritime Industry


Transportation stands as a major contributor to CO2 emissions globally, playing a pivotal role in the escalating challenge of climate change and global warming. As world leaders emphasize the imperative to limit global warming to 1.5°C, particularly in the wake of United Nations Climate summits, the transportation sector is under immense pressure to align with stringent emission reduction goals. The global shipping industry, responsible for transporting up to 90% of commercial goods, plays a vital role in world trade. However, this crucial trade is marred by its highly polluting nature, as ships predominantly rely on some of the most carbon-heavy fuels to power their engines. The result is the emission of a staggering 1,000 Mt of CO2 annually, which accounts for 3% of global CO2 emissions. Alarmingly, The International Maritime Organization warns that shipping emissions are poised to increase by up to 50% by mid-century if stringent measures are not promptly implemented. Of all these practices, coal usage stands out with the highest positive correlation with CO2 production, inflicting the most harmful environmental impact.

Future Solutions:


Carbon Capture Priorities With the global population set to double by 2050, forecasting energy demand and CO2 emissions is vital. It's crucial for informed policy and combating transportation-related emissions. Electric vehicles are transforming terrestrial transportation, reducing fossil fuel dependency. However, the maritime industry faces challenges without practical alternatives, highlighting the urgent need to address emissions in this sector. The maritime industry faces unique challenges in reducing emissions, primarily from burning heavy fuels. Unlike land-based transportation, there's no one-size-fits-all solution. Research into cleaner propulsion like hydrogen and ammonia is ongoing, but maritime adoption of sustainable alternatives lags. This emphasizes the need for innovative, internationally collaborative solutions to make shipping more environmentally friendly.

OUR SOLUTION:

Our innovative approach to addressing carbon emissions involves recycling CO2 through the engineering of E. coli K12 to produce the biofuel isobutanol. By integrating advanced hardware technologies, we aim to enhance the efficiency and scalability of engineered E. coli, creating a powerful system.

Enzymatic Solutions:


Engineering CO2 Fixation Bacteria: The genetic expression of key enzymes, including RuBisCO, Prk, AdhA, and KivD, plays a crucial role in our research. We focus on leveraging the Calvin-Benson cycle and E. coli's native pentose phosphate pathway to convert inorganic carbon into organic carbon, utilizing the Xylose substrate. Engineering Prk and Rubisco enables the reconstruction of the pathway, converting CO2 into isobutanol through the Glycolysis and Isobutanol pathways.

Hardware:


Efficient carbon capture is achieved through an advanced capture system designed for trapping CO2 emissions from flue gases. The captured CO2 is then directed to a cutting-edge bioreactor, where the transformation into isobutanol occurs. This unique process harnesses the Internet of Things (IoT) and a strategic alliance with Nautical Nexus, ensuring compatibility and sustainability in the maritime sector. Our adaptable system requires minimal adjustments and can be customized for a range of industries beyond maritime. Its versatility makes it an attractive solution for sectors seeking eco-friendly alternatives to reduce their carbon footprint. Whether in manufacturing, energy production, or other industrial applications, the system offers a promising avenue for addressing environmental concerns.

Isobutanol:


Isobutanol's compatibility with biodiesel and fossil diesel is a key advantage. By incorporating isobutanol into fuel blends, we create a more efficient and environmentally friendly energy source. The clean-burning properties of isobutanol can significantly reduce emissions in internal combustion engines, crucial for combatting climate change in transportation and industrial sectors.

Towards Sustainable Practices:


Constantly converting CO2 into biofuel through our innovative system represents a monumental stride towards sustainability. Isobutanol, combined with other fuels and advanced carbon capture technologies, can play a pivotal role in transitioning towards a future with virtually eliminated pollution. This approach aligns with global efforts to address environmental concerns and promote sustainable practices across diverse industries.


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