| Name | Type | Length | Description |
|---|---|---|---|
|
Promoter |
59bp |
PncA |
|
|
RBS |
12bp |
RBS |
|
|
Coding |
631bp |
EL222,acts as a repressor, blocking the binding of RNA polymerase and repress gene expression in the presence of blue light |
|
|
Terminator |
80bp |
It is used as the terminator of our protein EL222 in photosensitive switch. |
|
|
Promoter |
60bp |
Blue light repressible promoter with spacer addition |
|
|
Coding |
484bp |
With the function of N-acetyltransferase in Lactobacillus plantarum L168 |
|
|
Terminator |
129bp |
double terminator |
|
| Name | Type | Length | Description |
|---|---|---|---|
|
Composite |
718bp |
A photosensitive switch.This part allows constitutive production of the light-sensitive bacterial photosensitive protein EL222, a blue light activated transcription factor. This part could prove useful in the design of optogenetic (blue-light) circuit to regulate protein expression |
|
|
Composite |
707bp |
N-acetyltransferase with photosensitive switch |
| Name | Type | Length | Description |
|---|---|---|---|
|
Promoter |
35bp |
constitutive promoter family member |
|
|
RBS |
32bp |
RBS |
|
|
Coding |
714bp |
It is a cold-inducible transcription factor. When the temperature is below 35℃, the activity of TCI will gradually rising |
|
|
Terminator |
30bp |
Terminator |
|
|
Promoter |
78bp |
L(3) is the promoter of CI. It consists of 3 operators(DNA binding sites) OL1, OL2, OL3. CI binding results in the repression of regulation. |
|
|
Promoter |
100bp |
pR(3) is the promoter of CI. It consists of 3 operators(DNA binding sites) OR1,OR2,OR3. CI binding results in the repression of regulation |
|
|
Coding |
639bp |
rate limiting enzyme of protoporphyrin IX in Lactobacillus plantarum |
|
|
Terminator |
30bp |
Terminator |
| Name | Type | Length | Description |
|---|---|---|---|
|
Composite |
881bp |
Temperature sensitive switch |
|
|
Composite |
970bp |
rate limiting enzyme of protoporphyrin IX with Temperature sensitive switch |
