For our Human Practice work, we have considered "Be Responsible, Be Respective, Be Responsiveness" as our guiding principles. Therefore, we designed our HP activity structure out of concerns of the aspects below:

WHO?

Who are the stakeholders?

Who are the potential victims of mycotoxin?

Who will be our endusers?

WHY?

Why are they our stakeholders?

Why is our project important?

HOW?

How can we protect potential victims from mycotoxin?

How can our project change the world?

How can synthetic biology help our project and help the world?

Based on the aspects we considered, we practiced activities such as education for almost all age groups, the evaluation of SDGs, the identity of stakeholders, and so on...

Finally, our Human Practice work has successfully performed our job as a bridge conecting NAU-CHINA and the society.

To answer the question of who and why, as well as proving our work is good and responsible for the world. We analyzed the background of the project deeply and explained the concrete problem we were solving. We brainstormed all the stakeholders and involved them in our table of public organization. In addition, we evaluated their request and their priority through a power-interest grid which helped us to design our project more conprehensively.

To answer the question of how. We built our education programme with the guidance of Confucius——Tailor the teaching methods to each individual's unique situation. We designed distinguished education methodology, suiting people from different age groups to arouse their interest to synthetic biology, as well as raising their awareness of mycotoxin food safety.

Wine holds a distinguished position as one of the world's most popular beverages. According to the International Vine and Wine Organization(OIV), in 2022, 25.8 billion litres of wine were produced and consumed in total 23.2 billion litres. However, the European Food Safety Authority declares that wine ranks as the second-largest source of exposure to Ochratoxin.

Ochratoxin is another deadly fungal mycotoxin that has attracted widespread attention around the world after Aflatoxin. Ochratoxin A has the highest toxicity, the widest distribution, the highest virulence, the heaviest pollution of agricultural products, and the closest relationship with human health. Its trace concentration would cause severe carcinogenicity and neurotoxicity. Additionally, Ochratoxin A is classfied as a class 2B carcinogen by the International Agency for Research on Cancer (IARC) and one of the most toxic mycotoxins commonly fpund in food by the World Health Organization (WHO).

Thus, the accurate determination and degradation of OTA in wine is crucial for food safety.

Currently, the methods for detecting OTA include instrumental analysis and immunoassay. Instrumental analysis methods include gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and high-performance liquid chromatography (HPLC) and so on. However, these methods are expensive, time-consuming, and inconvenient. Immunoassay methods such as Enzyme-Linked Immunosorbent Assay (ELISA) and Immunochromatographic Assay (ICA) are more convenient, sensitive, and easy to operate, but they are also costly and susceptible to environmental influences.

We designed two modules for detecting and degrading OTA. The detecting module based on OTA and OTA aptamer binding, with high affinity and specificity, which aflicates signals through a structure-switching locked hairpin triggered RCA with assistance of dumbbell template so that we can detect it conviniently. The degradation module could convert OTA into phenylalanine and OTα that can be regarded as non-toxic to humans, which uses functional semi-interpenetrating polymer networks automatically assembled by probiotics to fuse OTA degrading enzyme M-CPA to form a living functional material.

For detailed information please click on the https://2023.igem.wiki/nau-china/description.

According to the participatory modelling principles provided by Alexey Voinov from Modelling with stakeholders, we brainstormed scientifically with the whole team. With our discussion, we classified stakeholders to categories below:

Academedia

Academedia includes universities and research institutions that are studying on grape or wine industry, and mycotoxin. These stakeholders have provided us countless help and suggestions.

Industry

Industries include wine factories, wine companies and other wine related organizations. They are the end users of BISTRO, so their voices should be considered carefully.

Government

There are numerous government departments focusing on the issue of mycotoxin. They are trying to save people from the threats of mycotoxin.

Healthcare

Healthcare includes the hospitals, departments, and organizations focusing on the body damage caused by mycotoxin.They have provided us with a lot of data to enrich the background for BISTRO.

Customer Community

Customer community includes everyone buying and drinking wine. If wine has became mildewed, they are first to suffer. So raising customers' awareness of avoiding mycotoxin is very important.

Public Organization

Public organization includes every EPO, NGO, and unofficial organizations built by citizens.

On this page, we sought to demonstrate that the BISTRO project by NAU-CHINA thoroughly considered reflection, responsibility, and responsiveness in its implementation.

At the project's outset, the entire team engaged in a brainstorming session to identify stakeholders. We classified these stakeholders, evaluated their requests, and prioritized them using a power-interest grid. Our project design was then developed based on their identified needs.

The Education and communication page illustrated how we connected our project with the world through a series of outreach activities. Using our innovative educational tools, we engaged numerous people covering almost all age groups in the enlightenment of synthetic biology, gaining valuable insights in the process. Through this two-way learning, we engaged in discussions with the public about the science behind synthetic biology and public values such as food safety and hunger. We meticulously recorded our approaches, evaluated and summarized them, with the hope of assisting other teams in continuing education and communication based on our work.

To reach a sustainable and harmonious future, we considered the contributions of our project towards the United Nations Sustainable Goals.

Our project BISTRO met SDG#2 Zero Hunger, and SDG#3 Good Health and Well-being.

For detailed information please click on the Sustainable developments page.

To confirm that BISTRO was responsible and good for the world, we needed suggestions and reflections from others. Therefore, NAU-CHINA actively participated in various collaborative events to exchange ideas. Thanks to collaborative universities such as LZU-CHINA, BIT-CHINA, SDU-CHINA, and NJMU-China, and iGEMer leagues like Nanjing iGEM Association (NIA), Conference of China iGEMer Community (CCiC), BISTRO improved significantly.

Moreover, education was a significant concern for iGEMers. We expressed our gratitude to those who collaborated with NAU-CHINA on different projects and looked forward to working jointly with them in the future to promote synthetic biology and the iGEM competition throughout China.

For detailed information please click on the Collaboration page .

ICII — Into China into iGEM, was an event sponsored and organized by Chinese participants since 2021. ICII last year was initiated by NAU-CHINA, which built an online synthetic biology education platform on the internet.

Last year, there were 4 teams: NAU-CHINA, LZU-CHINA, SDU-CHINA, and BIT-CHINA participating in ICII. A brief project introduction, activity plan, and the public values of each team were uploaded to www.icii.store as a newsletter.

For detailed information please click on the ICII page .

In order to meet all stakeholders' demands, we managed to design a better project which was more convenient, more efficient and cheaper. We adjusted the design of our project according to the stakeholders' suggestions duely and reflected what effect our project would exert on the world.Taking all the influencing factors into thoughtful consideration, we endeavoured our best to connect our project to the world and made it responsible and good for the world.

Hopefully, BISTRO will be good and responsible for the world. Product BISTRO can make wine industry better and better, and with the education project, people could rise their awareness of mycotoxin.