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This page serves as a comprehensive repository for all the experimental protocols utilized throughout the course of our investigation. It encompasses a detailed compilation of the methodologies and procedures employed in our project. For an in-depth exploration and access to the intricate lab notebooks associated with each experiment, kindly refer to the dedicated lab notebook page, where a wealth of specific and detailed information can be found.

Colony PCR

Competent Cells Preperation

Gel Electrophoresis

Gel Extraction

Golden Gate Assembly

Kanamycin Culture Plate Preperation

Plasmid Purification

Nanodrop

Oligonucleotide Resuspension Protocol

PCR Purification

PCR using NEB Q5 Polymerase

Restriction Digestion

Transformation

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