A total of 9 Basic parts have been added to the registry. 5 Composite parts have been designed on multiple combinations of the new basic parts and the previously used parts. The new basis parts that were designed are compatible with the RCF1000 assembly strategy making them suitable for Golden Gate and MoClo assembly techniques.
Previous parts used
| Biobrick ID | Part Type | Length(bp) | Description |
|---|---|---|---|
| http://parts.igem.org/Part:BBa_J23100 | Promoter | 35 | constitutive promoter family member |
| http://parts.igem.org/Part:BBa_B0030 | RBS | 15 | RBS.1 (strong) -- modified from R. Weiss |
| http://parts.igem.org/Part:BBa_B1007 | Terminator | 40 | Bidirectional Terminator. |
| http://parts.igem.org/Part:BBa_K2406020 | Promoter | 69 | T7 IPTG inducible promoter. |
| http://parts.igem.org/Part:BBa_K731721 | Terminator | 48 | T7 terminator. Wild type terminator from T7 Bacteriophage. |
| http://parts.igem.org/Part:BBa_K1537017 | T2A linker | 63 | T2A self-cleaving peptide sequence |
| https://parts.igem.org/wiki/index.php?title=Part:BBa_B0040 | Spacer | 70 | Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the BioBrick part's prefix containing the new gene's promoter. |
| https://parts.igem.org/Part:BBa_J428326 | Plasmid Backbone | 3359 | sfGFP reporter device for JUMP plasmids with flanking BsaI sites |
New Basic Parts
| Biobrick ID | Part Type | Length(bp) | Description |
|---|---|---|---|
| http://parts.igem.org/Part:BBa_K4836000 | CDS | 1497 | CUW_0748: Bacterial analogue of Human Serotonin Transporter |
| http://parts.igem.org/Part:BBa_K4836003 | CDS | 623 | The Coding sequence for Serotonin N-Acetyl Transferase (SNAT) |
| http://parts.igem.org/Part:BBa_K4836004 | CDS | 1094 | The coding sequence for Caffeic acid O-Methyl Transferase (COMT) |
| http://parts.igem.org/Part:BBa_K4836005 | CDS | 711 | mCherry Reporter CDS |
| http://parts.igem.org/Part:BBa_K4836006 | CDS | 702 | TagBFP CDS |
| http://parts.igem.org/Part:BBa_K4836008 | CDS | 720 | LasR Transcription Activator CDS |
| http://parts.igem.org/Part:BBa_K4836009 | Promoter | 131 | pLasI inducible promoter |
| http://parts.igem.org/Part:BBa_K4836010 | CDS | 1320 | Serotonin N-Acetyl Transferase (SNAT)+ TagBFP fusion protein |
| http://parts.igem.org/Part:BBa_K4836011 | CDS | 1794 | Caffeic acid O-Methyl Transferase (COMT)+ mCherry fusion protein |
New Composite parts
| Biobrick ID | Length(bp) | Description |
|---|---|---|
| http://parts.igem.org/Part:BBa_K4836007 | 897 | mCherry under pLasI Inducibe promoter |
| http://parts.igem.org/Part:BBa_K4836012 | 3274 | SNAT and COMT expression cassette with Fluorescent reporter proteins |
| http://parts.igem.org/Part:BBa_K4836013 | 1575 | LasR expression cassette. This cassette consists of LasR transcription factor that binds to serotonin and TagBFP |
| http://parts.igem.org/Part:BBa_K4836014 | 2388 | CUW_0748 expression cassette. This cassette consists of CUW_0748, the bacterial analogue of the human Serotonin Transporter and TagBFP |
| http://parts.igem.org/Part:BBa_K4836015 | 146 | The composite part consists of Inducible promoter pLasI and RBS |
| http://parts.igem.org/Part:BBa_K4836016 | 3345 | Serotonin induced regulatory cassette. This cassette examines the activation of the pLasI promoter when exposed to the Serotonin+LasR complex. |
