Transient Transformation Validation in Tobacco

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Transient Validation of NCED3-RUBY Reporting System

pDR5-RUBY plasmid available in our advisor's laboratory, we successfully constructed the red drought warning plasmid, Pro:NCED3-RUBY.

Transient Validation of GLR2.9-Bx Reporting System

To validate the functionality of the GLR2.9-Bx reporting system, we transformed the target plasmid into Agrobacterium and then transiently transformed tobacco leaves. Subsequently, we subjected them to stress treatment and recorded the changes in leaf color.

In addition to observing changes in leaf color, we also conducted fluorescence irradiation observations. The results showed that the stressed leaf areas produced green fluorescence under blue light, while other areas remained red.

Stable Transformation Validation in Arabidopsis

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Stable Validation of NCED3-RUBY Reporting System

To confirm the long-term stable expression of the NCED3-RUBY reporting system in plants, we entrusted the plasmid to a professional company for stable transformation and obtained transgenic seeds. Despite facing aphid damage during the seedling stage, preventing us from conducting formal stress experiments, we successfully cultivated these plants to maturity. Following drought treatment, we observed that the entire plants turned completely purple-red. This clear phenomenon indicates the effectiveness of our reporting system.

Future Plans

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Our future plans include obtaining viable data from formal stable transformation experiments and further optimizing system performance. We will continue to monitor the growth and color changes of these plants and regularly record relevant data. Additionally, we plan to conduct stress experiments under different drought conditions to comprehensively assess the system's performance and stability. Through these efforts, we will continuously refine our research and provide powerful tools for monitoring and studying plant responses to drought.