30
Sep
2022
November
November 10th
Brainstorming
December
December 1st
Check the project and the members
2023
March
March 10th
Finish our exams and be ready to start our experiments.
June
June 22 to June 28
PCR amplification of GLR2.9, enzyme digestion of the NCED3-RUBY plasmid, CEII ligation, verification of PCR products, and investigation of the reasons for band diffusion. Transformation of DH5α and culturing of colonies on plates.
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July
July 7 to July 15
Meeting with human practice supervisors: Brainstorming, integration to the team.
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July 16 to July 31
Receipt of NCED3-RUBY plasmid seeds, initiation of plant experiments, preparation of 1/2 MS solid medium, initial planting trials, T4 ligation, extraction of the basta plasmid, enzyme digestion, and bacterial shaking. Re-amplification of GLR2.9, extraction of BX fragments, and shaking with Agrobacterium.
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Augest
August 1 to August 15
Plant experiments including tobacco transplantation, Agrobacterium transformation into tobacco leaves, and drought experiments. Molecular experiments including validation of GLR2.9 PCR systems, construction of GLR2.9-RUBY systems, and colony PCR validation for E. coli and Agrobacterium.
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August 16 to August 30
Plant experiments involving the planting of NCED3-RUBY seeds for drought validation and tobacco transplantation. Molecular experiments including sequence validation by PCR, enzyme digestion, ligation to construct NCED3-Bx, preparation of permeation buffer, E. coli transformation, shaking, and plasmid extraction for NCED-Bx validation, as well as GLR2.9-RUBY-related activities.
Click here to see our notebook.
September
September 1 to September 27
Molecular Experiments:Agrobacterium Transformation and Verification in Various Systems Plant Experiments: Agrobacterium infiltration, leaf injection, simulation of Drought Experiment, time-lapse Photography.
Click here to see our notebook.