Our protocols includes

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DNA Purification from PCR.

DNA Extraction from Gel Electrophoresis.

Plasmid Extraction.

The PCR Reaction of DNA.

Restriction Endonuclease Enzymatic Shearing of DNA.

1% Agarose Gel Electrophoresis.

1.2% Agarose Gel Electrophoresis.

Agrobacterium Invertion of Tobacco Plant.

Heat Stimulated Invertion of E.coli.

Leaf Discoloration and Observation.

Ligation with T4 Ligase.

Ligation with ExnaseII.

Overnight Bacterial Culture.

Recepies Used in Experiments.