Primer List

This page lists the primers we use in our project and their sequences. We give every primer a number so that we could cite them in the wiki easily. For primers registered as parts, please see Parts page.


Copy Number Optimization Module

Number Name Sequence
1-1 pSA/pCS-BamHI-F NNNGGATCCCATGGTACGCGTGCTAGAGGCATCAAATAAAAC
1-2 pSA/pCS-KpnI-R NNNGGTACCTTTCTCCTCTTTAATGAATTCGGTCAGTGCGTCC
1-3 pET-BamH I-F NNNGGATCCCATGGTACGCGTG
1-4 pET-KpnI-R NNNGGTACCTTTCTCCTCTTTAATGAATTCGGTC
1-5 AR010-adh6-KpnI-F NNNGGTACCATGGCACCTGTTACAATTGAAAAGTTCGTAAATCAAGAAGAACG
1-6 AR010-adh6-BamHI-R NNNGGATCCTTAGTCTGAAAATTCTTTGTCGTAGCCGACTAAGGTAAATCTATATCT
1-7 pgm-galu-KpnI-F NNNGGTACCATGGCAATCCACAATCGTGCAGGC
1-8 pgm-galu-BamHI-R NNNGGATCCTTACTTCTTAATGCCCATCTCTTCTTCAAGCCAGGCTTTAAA
1-9 UGT-KpnI-F NNNGGTACCATGGGTAGCCAGATC
1-10 UGT-BamHI-R NNNGGATCCTTAATCCTGAGATTTCTGGCC
1-11 adh6-CF(KB) TTGGCTGCTCCACTATTATGTGGT
1-12 ugt85a1-CF(KB) AACTTCGGCAGCATCACTGTTC
1-13 galu-CF(KB) AGTGGGTGATGAACCGGTAGC
1-14 pSA-CR(KB) CTATCAGTGAAGCATCAAGACTAACAAACAAAAG
1-15 pET-CR(KB) TTCGCGTTAAATTTTTGTTAAATCAGCTCATTTT
1-16 pCS-CR(KB) ACCTGCCCATTCGACCACC
1-17 pETDuet-AR010-F TTTTGTTTAACTTTAAGAAGGAGATATACCATGGCACCTGTTACAATTGAAAAGTTCGT
1-18 pacyc/pCDF-AR010-F TTTTGTTTAACTTTAATAAGGAGATATACCATGGCACCTGTTACAATTGAAAAGTTCGT
1-19 gibson-AR010-adh6-R ATACGATTACTTTCTGTTCGACTTAAGCATTAGTCTGAAAATTCTTTGTCGTAGCCGAC
1-20 gibson-AR010-rPCR-F TGCTTAAGTCGAACAGAAAGTAATCGTATTGTACAC
1-21 pacyc/pCDF-AR010-rPCR-R GGTATATCTCCTTATTAAAGTTAAACAAAATTATTTCTACAGGGG
1-22 pETDuet-AR010-rPCR-R GGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGG
1-23 gibson-UGT-F TATTAGTTAAGTATAAGAAGGAGATATACATATGGGTAGCCAGATCATCCATAACTCTC
1-24 gibson-UGT-R TATTGCTCAGCGGTGGCAGCAGCCTAGGTTAATTAATCCTGAGATTTCTGGCCCAGC
1-25 gibson-UGT-rPCR-F TTAACCTAGGCTGCTGCCACC
1-26 gibson-UGT-rPCR-R ATGTATATCTCCTTCTTATACTTAACTAATATACTAAGATGGGGAA
1-27 gibson-AR010-rPCR-F(new) ACTAATGCTTAAGTCGAACAGAAAGTAATCGTAT
1-28 pETDuet-AR010-rPCR-R(new) GTGCCATGGTATATCTCCTTCTTAAAGTTAAACAAAA
1-29 pacyc/pCDF-AR010-rPCR-R(new) GGTGCCATGGTATATCTCCTTATTAAAGTTAAACAAAAT
1-30 gibson-UGT-rPCR-R(new) CTGGCTACCCATATGTATATCTCCTTCTTATACTTAACTAATA
1-31 gibson-UGT-R(new) GGTGGCAGCAGCCTAGGTTAATTAATCCTGAGATTTCTGGCCCAGC
1-32 aro-CR GGCCCTTTAAAATTTGAATCATGTAGCTGT
1-33 ugt-CF CGTTAAACAGCTGGTTGAGTTCGC
1-34 pacycduet-ugt-CR GTTGGACGAGTCGGAATCGC
1-35 pacycduet-AR010-CF CGCCGGAACATTAGTGCAGG
1-36 pCDFDuet-CR GGTACAGCGCAGTAACCGG
1-37 pCDFDuet-CF AGCCCACTGACGCGTT
1-38 pETDuet-UGT-CR CCGAGATAGGGTTGAGTGTTGTTCC
1-39 pETDuet1-ARO-CF CCATCGCCGCTTCCACTTT

Gene Knockout Module

Number Name Sequence
2-1 pheA-P1-F AAACACATCTGATTAATCCACATATCATTCTGTCC
2-2 pheA-P2-R CACCTTTTCAAGTGTTGCCTTTTTGTTATCAATAAAAAAGG
2-3 pheA-P3-F AGGCAACACTTGAAAAGGTGCCGGATGATG
2-4 pheA-P4-R CATACCAATGGTTTCTGGAGCAAATT
2-5 pykA-P1-F ACGCATGAGTTGTATGAATTGTAGCG
2-6 pykA-P2-R GGCAACGTACGTAATACTCCGTTGACTGAAACAACC
2-7 pykA-P3-F GGAGTATTACGTACGTTGCCGGATGCGG
2-8 pykA-P4-R GTACTGGGGATATTATTTACCCGATCAG
2-9 pykF-P1-F CAAAAATCAAACAAAATCAGACAAATAACGCG
2-10 pykF-P2-R AAAAGCAATAGACAGTCTTAGTCTTTAAGTTGAGAAGG
2-11 pykF-P3-F TAAGACTGTCTATTGCTTTTGTGAATTAATTTGTATATCGAAGCGC
2-12 pykF-P4-R GAGCTGCGTCATCTTTAGCAGC
2-13 sgRNA-pheA-F GGTATAATACTAGTGGTATTTTCAATCGGTACGAGTTTTAGAGCTAGAAATAG
2-14 sgRNA-pheA-R TCTAGCTCTAAAACTCGTACCGATTGAAAATACCACTAGTATTATACCTAGGACTGA
2-15 sgRNA-pheA2-F GGTATAATACTAGTATATACCGAAAGTACGTCTGGTTTTAGAGCTAGAAATAG
2-16 sgRNA-pheA2-R TCTAGCTCTAAAACCAGACGTACTTTCGGTATATACTAGTATTATACCTAGGACTGA
2-17 sgRNA-pheA3-F GGTATAATACTAGTGTATATTCAATCTTCCAGTGGTTTTAGAGCTAGAAATAG
2-18 sgRNA-pheA3-R TCTAGCTCTAAAACCACTGGAAGATTGAATATACACTAGTATTATACCTAGGACTGA
2-19 sgRNA-pykA-F GGTATAATACTAGTGATACGGGAGGTCATCAGCGGTTTTAGAGCTAGAAATAG
2-20 sgRNA-pykA-R TCTAGCTCTAAAACCGCTGATGACCTCCCGTATCACTAGTATTATACCTAGGACTGA
2-21 sgRNA-pykA2-F GGTATAATACTAGTTTCTGACGGATACTGCCCAGGTTTTAGAGCTAGAAATAG
2-22 sgRNA-pykA2-R TCTAGCTCTAAAACCTGGGCAGTATCCGTCAGAAACTAGTATTATACCTAGGACTGA
2-23 sgRNA-pykA3-F GGTATAATACTAGTGGTCGCCAATTTCCACACCGGTTTTAGAGCTAGAAATAG
2-24 sgRNA-pykA3-R TCTAGCTCTAAAACCGGTGTGGAAATTGGCGACCACTAGTATTATACCTAGGACTGA
2-25 sgRNA-pykF-F GGTATAATACTAGTGAGCACCTGAAAGCGCACGGGTTTTAGAGCTAGAAATAG
2-26 sgRNA-pykF-R TCTAGCTCTAAAACCCGTGCGCTTTCAGGTGCTCACTAGTATTATACCTAGGACTGA
2-27 sgRNA-pykF2-F GGTATAATACTAGTTCTGGTGCACTGGTACCGAGGTTTTAGAGCTAGAAATAG
2-28 sgRNA-pykF2-R TCTAGCTCTAAAACCTCGGTACCAGTGCACCAGAACTAGTATTATACCTAGGACTGA
2-29 sgRNA-pykF3-F GGTATAATACTAGTATCGTGGTTGCTACTCAGGGGTTTTAGAGCTAGAAATAG
2-30 sgRNA-pykF3-R TCTAGCTCTAAAACCCCTGAGTAGCAACCACGATACTAGTATTATACCTAGGACTGA
2-31 sgRNA-pykF4-F GGTATAATACTAGTAAAGAACTGGCTCTGCAGAGGTTTTAGAGCTAGAAATAG
2-32 sgRNA-pykF4-R TCTAGCTCTAAAACCTCTGCAGAGCCAGTTCTTTACTAGTATTATACCTAGGACTGA
2-33 pykA-yx-CF GCTGGAAAAAATCCAGTCTCAGCT
2-34 pykA-yx-CR GGTATCGCTTTAACGCCGCC
2-35 pykF-yx-CF ACTGCGCTGAATGCGTTTATTGG
2-36 pykF-yx-CR GGGTGTATTCGCATGAATGCACC
2-37 pheA-yx-CF TGCCGCTGATGGAAAATGCAT
2-38 pheA-yx-CR GGGTGTACCGCCAGATCTGATT
2-39 pCAS-R GTCTTTCCTCAATCATCTCCCTATCTTCAAATAAGG
2-40 pCAS-F TGAGGGAGATTTAAATCCTGATAATAGTGATGTGGA
2-41 pTargetF-CF GCGGTATTTTCTCCTTACGC
2-42 pTargetF-CR GCAGGTCGACTCTAGAGAAT

Fusion Expression Module

Number Name Sequence
3-1 (ADH6+AR010)-P1 GAGAAAGGTACCATGTCTTATCCTGAGAAATTTG
3-2 (ADH6+AR010)-P2-(1) GGAGCCGCCGCCGCCGTCTGAAAATTCTTTGTCGT
3-3 (ADH6+AR010)-P2-(2) GGAGCCGCCGCCGCCGGAACCACCACCACCGGAGCCGCCGCCGCCGTCTGAAAATTCTTTGTCGT
3-4 (ADH6+AR010)-P2-(3) ACCACCACCACCGCCGCCGCCGCCGTCTGAAAATTCTTTGTCGT
3-5 (ADH6+AR010)-P2-(4) TTTAGCAGCAGCTTCGTCTGAAAATTCTTTGTCGT
3-6 (ADH6+AR010)-P2-(5) TTTAGCAGCAGCTTCTTTAGCAGCAGCTTCTTTAGCAGCAGCTTCGTCTGAAAATTCTTTGTCGT
3-7 (ADH6+AR010)-P2-(6) CGGAGCCGGAGCCGGGTCTGAAAATTCTTTGTCGT
3-8 (ADH6+AR010)-P3-(1) GGCGGCGGCGGCTCCATGGCACCTGTTACAATTGA
3-9 (ADH6+AR010)-P3-(2) GGCGGCGGCGGCTCCGGTGGTGGTGGTTCCGGCGGCGGCGGCTCCATGGCACCTGTTACAATTGA
3-10 (ADH6+AR010)-P3-(3) GGCGGCGGCGGCGGTGGTGGTGGTATGGCACCTGTTACAATTGA
3-11 (ADH6+AR010)-P3-(4) GAAGCTGCTGCTAAAATGGCACCTGTTACAATTGA
3-12 (ADH6+AR010)-P3-(5) GAAGCTGCTGCTAAAGAAGCTGCTGCTAAAGAAGCTGCTGCTAAAATGGCACCTGTTACAATTGA
3-13 (ADH6+AR010)-P3-(6) CCGGCTCCGGCTCCGATGGCACCTGTTACAATTGA
3-14 (ADH6+AR010)-P4 CGTACCATGGGATCCTTATTTTTTATTTCTTTTAAGTGC
3-15 (ADH6+AR010)-CF(yz) GGTCTTGGTGGTATCGGCAGTATG
3-16 (ADH6+AR010)-CR(yz) CCGAGCAAGCGACTCTATCTTTTAC

Gene Integration Module

Number Name Sequence
4-1 pDonor-AR010-F CGAGAACAGGGGCTGGTGAAATGGCACCTGTTACAATTGAAAAGTTCGTAAA
4-2 pDonor-AR010-R GCTGTGTATAAGGGAGCCTGACATTTATTTTTTATTTCTTTTAAGTGCCGCTGCTTC
4-3 pDonor-UGT-F CGAGAACAGGGGCTGGTGAAATGGGTAGCCAGATCATCCATAACTC
4-4 pDonor-UGT-R GCTGTGTATAAGGGAGCCTGACATTTAATCCTGAGATTTCTGGCCCAGC
4-5 pDonor-rPCR-F ATGTCAGGCTCCCTTATACACAGC
4-6 pDonor-rPCR-R TTCACCAGCCCCTGTTCTCG
4-7 pDonor-AR010-F(new) GACATTTTCATGAAGAGGTCATTTCGGACATGGCACCTGTTACAATTGAAAAGTTCGTAAA
4-8 pDonor-AR010-R(new) TTTCAGCAAAACTACTGCAGTAAGCAACTTATTTTTTATTTCTTTTAAGTGCCGCTGCTTCA
4-9 pDonor-UGT-F(new) GACATTTTCATGAAGAGGTCATTTCGGACATGGGTAGCCAGATCATCCATAACTC
4-10 pDonor-UGT-R(new) TTTCAGCAAAACTACTGCAGTAAGCAACTTAATCCTGAGATTTCTGGCCCAGC
4-11 pDonor-rPCR-R(new) GTCCGAAATGACCTCTTCATGAAAATGTC
4-12 pDonor-rPCR-F(new) GTTGCTTACTGCAGTAGTTTTGCTGAAA
4-13 pDonor-AR010-R(yz) AGACATATCCGCAAAATCTGCAGG
4-14 pDonor-UGT-R(yz) GGTGGTACGGATGAAAGACGGA
4-15 B28T10-F(yz) TAAAACATCACTTCCCGCAGGCG
4-16 B28T10-R(yz) AAAATATCGGCTACAGCGAAAACGAC
4-17 pCutamp-CF GCCATCAACTCGGCAAGATGC
4-18 pCutamp-CR CGGCGACGTTTAAGCTGTTTC
4-19 pCutamp-CF(2) CGGCATTTCCCATATTACACGCC
4-20 pCutamp-CR(2) TCGTTAACGGAAGCGTAGGAGT
4-21 pDonar-CF(yz) AGTGAGCTGATACCGCTCGC
4-22 pDonar-CR(yz) TGGCTTAACTATGCGGCATCAG
4-23 pDonar-CF CCACGCTTCCCGAAGGGA
4-24 pDonar-CR GGCGAGTTACATGATCCCCCA
4-25 pEffect-CF GCCATACCGCGAAAGGTTTTGC
4-26 pEffect-CR GCACCTCTAACAACCGCGC

RNA Thermometer Module

Number Name Sequence
5-1 EGFP-Kpn I-F NNNNGGTACCATGGTGAGCAAGGGCGAGG
5-2 EGFP-BamHI-R NNNNGGATCCTTACTTGTACAGCTCGTCCATGCCG
5-3 U6(1)-F AAAGAATTCTTCTCCTCACTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAGGAGAAAGGTACCATGG
5-3 U6(1)-F AAAGAATTCTTCTCCTCACTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAGGAGAAAGGTACCATGG
5-4 U6(1)-R CCATGGTACCTTTCTCCTTTTTTTTTTTTTTTTTTTTTTTTTTACTAGTGAGGAGAAGAATTCTTT
5-5 U6(2)-F AAAGAATTCTTCTCCTCACTAGTAAAAAAAAAAAAAAAAGGAGAAAGGTACCATGG
5-6 U6(2)-R CCATGGTACCTTTCTCCTTTTTTTTTTTTTTTTACTAGTGAGGAGAAGAATTCTTT
5-7 U7-F AAAGAATTCTACTCGGTCTCCTCAGAGGAGAAAGGTACCATGG
5-8 U7-R CCATGGTACCTTTCTCCTCTGAGGAGACCGAGTAGAATTCTTT
5-9 U8-F AAAGAATTCTACTCGGTCTCCTAGAGGAGAAAGGTACCATGG
5-10 U8-R CCATGGTACCTTTCTCCTCTAGGAGACCGAGTAGAATTCTTT
5-11 U9-F AAAGAATTCTACTCGGTCTCCTACTAGTCTGCAGAGGAGAAAGGTACCATGG
5-12 U9-R CCATGGTACCTTTCTCCTCTGCAGACTAGTAGGAGACCGAGTAGAATTCTTT
5-13 U6(new)-F AAATCTAGATTCTCCTCACTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAGGAGAAAGGATCCATGG
5-14 U6(new)-R CCATGGATCCTTTCTCCTTTTTTTTTTTTTTTTTTTTTTTTTTACTAGTGAGGAGAATCTAGATTT
5-15 U6-2(new)-F AAATCTAGATTCTCCTCACTAGTAAAAAAAAAAAAAAAAGGAGAAAGGATCCATGG
5-16 U6-2(new)-R CCATGGATCCTTTCTCCTTTTTTTTTTTTTTTTACTAGTGAGGAGAATCTAGATTT
5-17 U7(new)-F AAATCTAGATACTCGGTCTCCTCAGAGGAGAAAGGATCCATGG
5-18 U7(new)-R CCATGGATCCTTTCTCCTCTGAGGAGACCGAGTATCTAGATTT
5-19 U8(new)-F AAATCTAGATACTCGGTCTCCTAGAGGAGAAAGGATCCATGG
5-20 U8(new)-R CCATGGATCCTTTCTCCTCTAGGAGACCGAGTATCTAGATTT
5-21 U9(new)-F AAATCTAGATACTCGGTCTCCTACTAGTCTGCAGAGGAGAAAGGATCCATGG
5-22 U9(new)-R CCATGGATCCTTTCTCCTCTGCAGACTAGTAGGAGACCGAGTATCTAGATTT
5-23 pET28a-CF AGCCCGATCTTCCCCATCG
5-24 pET28a-CR TTGTCGGGCAGCAGCACG
5-25 pET-EGFP-CF(CX) GCCATTCGATGGTGTCCGGG
5-26 pET-EGFP-CR(CX) GCCGTTCTTCTGCTTGTCGG