Contribution

We are pleased to announce that we have successfully finalized the contribution page. We fully comprehend the significance of contributing to both the iGEM community and society as a whole.
1. As mentioned earlier, we have diligently documented several new parts on the iGEM public platform, which will prove invaluable for upcoming iGEM teams.
2. We have contributed genetically encoded and fluorescence-based intracellular NAD+ biosensor FiNad to the iGEM database.
3. We have contributed key plasmids for running the mammalian cell-based directed evolution system to the iGEM database.
4. By integrating any new reporter system other than FiNad, the future iGEM team will find it very easy to perform directed evolution to address their scientific questions.
Through these efforts, we aspire to create greater awareness and exposure for the field of synthetic biology among the general public.
For detailed information about these parts, please view the part page.


Parts

Name Nickname Type Description
BBa_K5005000 BFP Basic BFP downstream of T7 promoter.
BBa_K5005001 EGFP Basic EGFP downstream of T7 promoter.
BBa_K5005002 pTarget-BFP Basic The plamid backbone for BFP
BBa_K5005003 AID-T7 RNAP-UGI Basic Expresses editor under dox induction.
BBa_K5005004 NAMPT Basic AS PCR template for NAMPT fragment cloning.
BBa_K5005005 pCDH-CMV-T7-NAMPT Basic Primordial plasmid before point mutation.
BBa_K5005006 NAMPT-V221G Basic The product of NAMPT point mutation - V221G.
BBa_K5005007 NAMPT-V221A Basic The product of NAMPT point mutation - V221A.
BBa_K5005008 NAMPT-D354E Basic The product of NAMPT point mutation - D354E.
BBa_K5005009 NAMPT-G384C Basic The product of NAMPT point mutation - D384C.
BBa_K5005010 NAMPT-L386P Basic The product of NAMPT point mutation - L386P.
BBa_K5005011 pCDH-CMV-mCherry-FiNad Basic Plasmid for Express NAD+ biosensor- FiNad.
BBa_K5005013 FiNad Basic Express NAD+ biosensor- FiNad.
BBa_K5005014 pTarget Basic The plamid for BFP/EGFP.