Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.
Wet Lab:
General brainstorming about possible project ideas to discuss with Prof. Zaiss.
Wet Lab:
Meeting with Prof. Zaiss and discussing the ideas we came up with.
Finances:
First Meeting to discuss possible financing opportunities.
Human Practices:
Brainstorming about general project ideas.
Wet Lab:
Sorting through and categorize our ideas, rough first workflow.
Dry Lab:
First Discussion with Prof. Böckmann + new plan to use open source microbiome data and make comparison with wetlab results.
Wet Lab:
Searching for SCFAs receptors and experiments we could perform to detect their activity.
Human Practices:
Preparation of questions for the general public Pol and future Interviews + planning the podcast.
Human Practices:
Writing the first podcast script.
Wet Lab:
Collecting ideas to detect SCFAs: quantification with split GFP-assay or measure claudin unregulation or measure FFAR2 phosphorylation.
Wet Lab:
New idea: Microfluidics device to detect SCFAs via binding to respective enzymes.
Wet Lab:
Discuss research about microfluidics, biosensors, enzymes, plasmids.
Human Practices:
Deciding on initial pool of questions for the general public Pol.
Dry Lab:
Generating ideas, introducing the Human Microbiome project, research on IBD sources.
Dry Lab:
Sharing individual research, collecting ideas, deciding on a python schedule.
Human Practices:
Making the introduction sound for the podcast series.
Finances:
Starting the first draft of the grand application.
Wet Lab:
Discuss suitable enzymes, fluorochromes and optical biosensors.
Finances:
Distributing the different sections of the grand application.
Wet Lab:
Further research and discussing about suitable enzymes, on how to detect fluorescence and about plasmids for bacterial transformation.
Wet Lab:
Discussing and choosing operons for SCFA detection, selecting fluorochromes with no/little spectral overlap and considering which plasmid would fit our purpose.
Wet Lab / Finance:
Create presentation for biology department
Finances:
Presenting our topic and iGEM to the Biology Department.
Human Practices:
Translation of the general public Pol into German, Spanish and Turkish.
Wet Lab / Finance
Create presentation for chemistry department.
Finances:
Presenting our topic and iGEM to the Chemistry Department.
Dry Lab:
Meeting with Prof. Burkovski: Modelling part abandoned, omparison between the genome of two species to identify similar genes.
Meeting of Dry Lab Team with Wet Lab team to discuss how dry lab could integrate wetlab results.
Human Practices:
Release date of the general public Pol.
Brainstorming about potential collaboration ideas.
Preparation of a list with potential schools and games for public outreach/education.
Human Practices:
Recording and editing of the first podcast episode.
Wet Lab:
Gathering sequences for promotors and fluorochromes, sort experiments we will conduct and further plan plasmid design.
Wet Lab:
Meeting with Prof. Burkovski to clarify questions about plasmid.
Dry Lab:
Collecting ideas: Calculators, games, etc. + Distribution of research about specific genes.
Human Practices:
Designing and colouring games for the schools.
Finances:
Getting financial support for laboratory materials from Pandora.
Dry Lab:
Potential gene candidates discussed.
Human Practices:
Writing scripts for podcast episodes.
Establishing podcast subteams.
Human Practices:
Recording of podcast episodes.
Dry Lab:
Preparing questions for meeting with Prof. Burkovski.
Fiances:
Proof-Reading the grand application and starting to send it to various foundations/companies.
Wet Lab:
Task distribution: start designing our constructs i Benchling, find experiment protocols, write a list what materials we will need in the lab.
Fiances:
Sending away our application for the Team Impact Grand.
Wet Lab:
Create rough time plan for the lab work, ask Prof. Koch and Prof. Sonnewald for glnAp2 sequence and ask Prof. Winkler to provide E.coli.
Human Practices:
Preparing new scripts, recording and editing of the trailer for the podcast.
Human Practices:
Brainstorming about potential meetings/interviews with patients, experts and companies.
Wet Lab:
Discuss cloning methods and tags to save primer for PCR.
Human Practices:
Recording and editing of podcast episodes.
Wet Lab:
Create experiment schedule to plan experiments and double check our protocols.
Dry Lab:
Meeting with Prof. Burkovski.
Human Practices:
Contacting schools and pharmacological companies.
Wet Lab:
Introductio of the wet lab team to Prof. Slany, Prof. Nimmerjahn, Prof. Lux and TA, finalize Benchling constructs so IDT sequence can be ordered.
Human Practices:
Recording and editing the podcast.
Dry Lab:
Alternative idea: kegg pathway analysis + further research about kegg pathway.
Wet Lab:
Preparing LB agar plates.
Discussing next steps and what can be prepared while IDT sequences are ordered.
Human Practices:
Preparing a questionnaire for IBD patients + Interview script to talk with Prof. Zaiss.
Dry Lab:
Designing the primer calculator.
Dry Lab:
Contacting Prof. Sonnewald for scientific advice related to kegg pathway.
Wet Lab:
Meeting with Prof. Winkler to discuss our procedure in the lab.
Human Practices:
Meeting with Prof. Sonnewald.
Dry Lab:
Finishing and Documenting the primer calculator.
Wet Lab:
IDT DNA elution and first amplifying PCR.
Human Practices:
Outlines for the interview with Prof. Zaiss and Prof. Atreya.
Dry Lab:
Meeting with Prof. Sonnewald.
Wet Labs:
Gel electrophoresis to confirm PCR.
Wet Lab:
First Gibson Assembly and bacteria transformation.
Human Practices:
Recording a podcast episode.
Human Practices:
Recording + editing the next podcast episode.
Translating the IBD patient questionnaire to English and German.
Meeting of Dry Lab and Wet Lab about samples for mass spectrometry + Model preparation.
FAUgem trailer is aired.
Human Practices:
Recording podcast episodes.
FAUgem S1E1 is aired.
Dry Lab:
Finalizing the model.
FAUgem S1E2 is aired.
Human Practices:
Editing podcast episodes.