Describe how and why you chose your iGEM project.
| Part Number | Part Name | Part Origin | Type | Description/How was it used? | Original Author |
|---|---|---|---|---|---|
| BBa_K4345003 | mPapaya | Zoanthus sp.. | Reporter | This part codes for a bright yellow fluorescent protein, derived from Zoanthus sp.. More information can be found on our Contribution page | Luka Van den Berghe |
| BBa_K2616001 | RIP (RNAIII Inhibiting Peptide) | S. aureus | Protein Coding Region | This part encodes for the production of RIP, which is a heptapeptide originally thought to interfere with quorum sensing in S. aureus. However, upon further research of literature, supported by our own modelling, RIP may not work in the way originally thought, hence why we decided to target AI2 quorum sensing instead. More information can be found on our Contribution page. | iGEM Pasteur Paris 2018 |
| BBa_K4694002 | His-QQ-7 | Identified in a screen of bacteria that quench biofilm formation. | Protein Coding Region | Quorum Quencher 7 (QQ-7) has been shown to decrease biofilm formation of C. albicans and S. epidermidis. QQ-7 is proposed to interfere with the yeast-to-hyphal switch in C. albicans and to induce expression of the icaR gene encoding the repressor that prevents exopolysaccharide biosynthesis in S. epidermidis. | Exeter iGEM Team 2023 |
| BBa_K4694003 | Sp-His-QQ-7 | Identified in a screen of bacteria that quench biofilm formation. | Protein Coding Region | This part codes for QQ7- with an L. plantarum signal peptide (Lp_3050) at the N-terminal to allow for export from the cell . | Exeter iGEM Team 2023 |
| BBa_K4694004 | His-QQ-5 | Identified in a screen of bacteria that quench biofilm formation. | Protein Coding Region | Quorum Quencher 5 (QQ-5) has been shown to decrease biofilm formation S. epidermidis. QQ-5 is proposed to induce expression of the icaR gene encoding the repressor that prevents exopolysaccharide biosynthesis in S. epidermidis. | Exeter iGEM Team 2023 |
| BBa_K4694005 | His-LuxS | L plantarum WCFS1 | Protein Coding Region | LuxS catalyses the formation of (S)-4,5-dihydroxy-2,3-pentanedione (DPD). DPD is the precursor of autoinducer (AI)-2. AI-2 is a signal molecule which is involved in inhibiting biofilm formation of Gram-positive bacteria. | Exeter iGEM Team 2023 |
| BBa_K4694006 | His-AHL-Lactonase | Bacillus thuringiensis serovar kurstaki (29339) | Protein Coding Region | AHL-lactonase breaks down N-acyl homoserine lactones (AHLs) of Gram-negative bacteria into N-acyl-homoserine. | Exeter iGEM Team 2023 |
| BBa_K4694008 | Sp-His-AHL-Lactonase | Bacillus thuringiensis serovar kurstaki (29339) | Protein Coding Region | This part codes for AHL-lactonase with an L. plantarum signal peptide (Lp_3050) at the N-terminal to allow for export from the cell. | Exeter iGEM Team 2023 |
| BBa_K4694009 | Sp-His-QQ-5 | Identified in a screen of bacteria that quench biofilm formation. | Protein Coding Region | This part codes for QQ-5 with an L. plantarum signal peptide (Lp_3050) at the N-terminal to allow for export from the cell . | Exeter iGEM Team 2023 |
| BBa_K4694010 | His-CapA | L plantarum WCFS1 | Protein Coding Region | The enzyme CapA is a polysaccharide biosynthesis protein | Exeter iGEM Team 2023 |