Describe the research, experiments, and protocols you used in your iGEM project.
Dyslexia friendly
Media
Making LB
Making MRS media
Making BHI
Making TSB
Molecular Biology
Preparation of competent E. coli cells
Transformation of competent E. coli cells (heat-shocked based)
Preparation of competent L. plantarum cells
Transformation of competent L. plantarum cells
Plasmid DNA extraction
TypeIIS Cloning
Western Blot
Cell Lysis
Biofilms
Forming L. plantarum biofilms
Forming S. epidermidis biofilms
Forming P. fluorescens biofilms
Biofilm Washing and staining
Assays
Lysate and supernatant assays on biofilms
LCMS standard prep
LCMS enzyme assay