Overview

Our project, SCENTIPD, is centered around improving the diagnosis of Parkinson's disease (PD), an incurable neurodegenerative disorder that affects millions of individuals worldwide. While designing and creating our test kit, we were and still are strongly committed to ensuring its positive impact on society.

The core values of our project

First and foremost, the seriousness of Parkinson's Disease cannot be expressed strongly enough. PD significantly impairs the quality of life for those affected by it and places a substantial burden on healthcare systems and caregivers. Understanding the seriousness of the problem encouraged us to choose and develop this project. There is significant evidence that early diagnosis can be the key for better prognosis and quality of life. To that end, the urgency of detecting the disease before the onset of the first symptoms is obvious.

Our diagnostic test kit for Parkinson's Disease is proof of our dedication to protecting the quality of life of potential patients. By detecting PD a decade or more before its initial symptoms manifest, our kit offers individuals a critical window of opportunity. This early detection means they can be proactive, making it possible to seek timely medical intervention, engage in therapeutic treatments, adopt healthier lifestyles, and make informed decisions to significantly slow the progression of the disease. This empowers them to maintain their independence, mobility, and cognitive function, thus preserving their dignity and quality of life.

Ultimately, our kit aligns with the fundamental life values of compassion, offering hope to those who may face the challenges of PD in the future.

Additionally, our project is aligned with the values of safety and ethical responsibility. We respected the safety measures required for handling biological materials, and we made sure that our diagnostic kit adhered to the necessary safety standards. The ethical part of the test was also the center of our discussions, particularly concerning patient data privacy.

The people and communities we consulted

To ensure our project's responsibility, we met online and face-to-face, with various stakeholders, recognizing that the perspectives of each group were very important. We reached out to scientists such as doctors, biologists, biochemists, bioinformaticians, all of them experts in their fields, and of course elderly people and patients of Parkinson's Disease.

We aimed to create a diagnostic tool that could reach a wide range of patients, especially those in remote regions who may have limited access to advanced medical examinations and facilities. It is known that Greece is a country with a really diverse geography, and at the same time with nearly 25% of its population being over the age of 65 and their highest concentration being in rural areas. Our own grandparents live in such small villages, far away from towns where they can get proper diagnosis and treatment for whatever medical issue that they may have.

Our project aims to produce an accessible, cheap diagnostic point of care (POC) test that does not need any special equipment or specialized staff.

The people we met in our educational events

During our preparation for the competition, we organized several gatherings to educate and inspire, reaching out to various communities. Our educational events were designed to be both enlightening and unique, ensuring that participants of all backgrounds could engage with biology, synthetic biology, and science at large.

For instance, the biosafety debate and the theatrical games that followed, challenged conventional thinking about safety in scientific research, fostering deep and interesting discussions. Our Science Game Night mixed fun and learning, with interactive quizzes and science-themed adaptations of popular games, like TABOO, Pictionary and Pantomime. Hosting an open-air cinema screening of "Gattaca" encouraged thinking on the ethical dimensions of genetics. In the bioinformatics workshop, participants were introduced into the fascinating world of genomic data analysis. These events were more than just lessons; they were journeys into biology, synthetic biology, and science at large. They sparked curiosity, broadened horizons, and shed light on the complexity and cruciality of Parkinson's disease.

Inclusivity

Throughout our preparation for the iGEM competition, we were determined to overcome barriers to participation in synthetic biology and make science accessible to all. Our efforts reached various groups, including the elderly residents of "Sivilla" care home, children at "Smile of The Child" and "Children's Villages SOS" homes, and young refugees at "Metadrasi" association's educational center.

For the refugees, many of whom spoke French due to their origins in war-torn African countries, our team members who spoke adequately their language made an effort to communicate in their mother tongues, creating a sense of familiarity and connection. We recognized by our time with these kids that language plays a vital role in effective engagement.

In a school in Xanthi, where the majority of students were Pomakoi, a Muslim minority with their own unique language blending Greek and Turkish elements, we took the initiative to learn the basics of their language. This allowed us to bridge communication gaps and present some of our material in their language, ensuring that our slides in the PowerPoint presentation were understood by all.

Our educational sessions in all of these events, covered a wide range of scientific topics, from basic biology to cellular, molecular biology, and synthetic biology, but also microorganisms, such as bacteria, fungi and viruses. We prepared the material keeping in mind that it must be very simple and easily understandable, since the audience most likely had limited prior knowledge in these fields. Engaging activities like DNA extraction from bananas, visual aids featuring photographs of bacterial cultures in petri dishes, and science-themed games such as Taboo, Pantomime, and Pictionary made learning interactive and fun.

Moreover, our commitment to expanding access to synthetic biology extended to organizing a National Competition of Riddles in Synthetic Biology, inviting schools from all around Greece to participate, regardless of their location. This initiative aimed to inspire a broader audience, proving that science has no borders.

Lastly, we engaged in meaningful discussions with residents of remote villages, gaining insight into their medical needs and the challenges they continuously face in accessing healthcare. This dialogue helped shape our project to be more inclusive to diverse communities.

Impact

In conclusion, our project, SCENTIPD, is all about improving the lives of senior citizens and society at large. We engaged extensively with the interested parties, considered ethical and safety aspects, and aimed for broad accessibility. We strive to make a responsible and positive impact by addressing a pressing medical need, potentially transforming the landscape of PD diagnosis for the better. Simultaneously, we tried to share our knowledge on science, transcending traditional teaching methods. Through engaging events, we transformed science education into an interesting and interactive experience.

Synaesthesia

From the moment this bacteriophage-based colorimetric sensor was investigated as a possible solution for our goal of detecting Volatile Organic Compounds, a concept came to mind: Synaesthesia.

It is defined as a neuropsychological trait in which the stimulation of one sense causes the automatic experience of another sense. Synaesthesia is a genetically linked trait estimated to affect from 2 to 5 percent of the general population.

A common form of this trait is the association of sounds with colors. It is found frequently among musicians and other artists, often inspiring and helping with their creative work.

In our case, smell is associated with color.

And since anosmia, or loss of smell, is a symptom often accompanying Parkinson's Disease at an early stage, one can also see our project “bringing back” this lost sense.

Finally, since we mentioned art and sound, let us think about music. Here is a song that one of our members wrote during this year full of hardships, but also fulfilling and impactful moments.

Synaesthetic Love

Like a ship that can't leave port
Like a song that can't begin
Let me write this lab report
For this sorry state we're in

Overthinking our devotions
Not in business for new parts
Autoclaving our emotions
In the furnace of our hearts

Let our protocol resume
Leave no room for compromise
And the scent of your perfume
be the color for my eyes

Let our movements blur the line
Let us touch without the glove
All the senses to intertwine
For our synaesthetic love

Integrated Human Practices

Patients are at the core of our efforts; we want to provide them with a reliable, early diagnosis test to improve their quality of life. Doctors and neurologists benefit from a more efficient and accurate diagnostic process, ensuring that they have enough time to treat the patients, before severe loss of their dopaminergic neurons. At the same time, biologists and pharmaceutical companies can use our diagnostic kit for research purposes. Our diagnostic kit is designed to be user-friendly, allowing healthcare professionals to use it easily and effectively.

The team's approach to incorporating feedback and insights from a diverse range of experts and stakeholders throughout their project development is commendable. From the very inception of their project, they actively sought external perspectives and engaged in meaningful discussions with professionals from various fields, including neurology, biology, chemistry, and business. This inclusive and collaborative approach allowed them to not only validate the worthiness of their project idea but also to refine and optimize their diagnostic test kit for Parkinson's disease. Their engagement with experts, including representatives from pharmaceuticals, diagnostic centers, and environmental companies, demonstrated a strong commitment to understanding the existing landscape and potential for their project. This thorough research and dialogue helped them make informed decisions and navigate the complexities of their chosen endeavor. Furthermore, their interactions with the elderly population to gauge how their kit would impact them and ensure user-friendliness showcased a genuine concern for the end-users and a dedication to creating a solution that genuinely improves lives. The team's proactive approach to seeking advice and guidance from business experts not only enhanced their project's marketability but also resulted in their successful win in the entrepreneurship MindSpace Competition for life sciences. This achievement underscores the tangible benefits of their well-implemented Human Practices work. Overall, their comprehensive and well-documented approach serves as a valuable example for others embarking on similar projects, highlighting the importance of multidisciplinary collaboration and stakeholder engagement in creating responsible and impactful solutions for real-world challenges.

Our team's integration of Human Practices work throughout our project was constant. We showcased a meticulous and thoughtful approach by actively engaging with a wide range of experts and stakeholders from the very inception of their project. This engagement was not only well-documented but also exhibited a genuine commitment to learning, collaborating, and adapting their project based on feedback and insights. The team's approach serves as an inspiring example for others in the synthetic biology community and beyond. It highlights the importance of seeking external perspectives, especially from experts in relevant fields, and demonstrates how this can significantly shape the development of a responsible and impactful project. Their documentation of this extensive engagement is thorough and well-structured, making it accessible for others to learn from and build upon. Their rationale and prior work are clearly explained, providing valuable context for their decision-making process. The incorporation of different stakeholder views, including those of experts, potential end-users (the elderly), and business experts, was a prominent feature of their project. This inclusive approach ensured that their diagnostic test kit for Parkinson's disease addressed the needs and concerns of various parties. Overall, the team convincingly demonstrated that their Human Practices activities played a pivotal role in creating a project that is not only technically innovative but also responsible and geared toward improving the lives of individuals at risk of Parkinson's disease. Their successful win in the entrepreneurship MindSpace Competition further underscores the tangible impact of their approach in making their project a reality.

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OUR ADVENTURE

Workshop on Climate Change and Green Growth

Space Biotechnology Webinar

Verde Tec

"Panorama" Entrepreneurship Fair

Scientific Congress of Medical Students of Greece

Panhellenic Symposium of College Students for Bioscientists

Pharmaceutical Conference of Pharmaceutical College Students

Chemexpo

Career and Mobility Fair @ Erasmus Leaders

iGEM Start Up Summer School

Panhellenic Conference of the Hellenic Neurological Society

Online Meeting with Parkinson's Union, a Union of Parkinson's Patients in Athens

On the 9th of September 2023, our team had the privilege of engaging in a meaningful virtual dialogue via Zoom with members of the Parkinson's Association in Athens, the largest Parkinson's disease association in Greece. This enlightening interaction provided a unique window into the lives of individuals who are navigating the challenging terrain of Parkinson's disease, which of course is the core of our project.

During these conversations, we attentively listened to their life narratives, which were marked by the shocking moments of their diagnosis, the age at which the first symptoms manifested, and the subsequent impact on their daily lives. We discussed whether they had the disease in their families, to understand how often PD is due to genetics and inheritance. They also informed us of changes and adjustments they made in dietary habits and daily routines, and of the people around them, whether relatives or friends, that support them.

We also inquired about their current symptoms, ongoing medications, and the proactive measures they employ to slow down the progression of this incurable disease. We questioned whether they would have liked to have known earlier that they have Parkinson's, which is what our kit aims to do, diagnose the illness early. They responded that yes, they would have wanted to know, since it would save them many years of searching for the right diagnosis and treatment. They had difficulties on what doctor to consult and what medical exam to do, from orthopedic specialists to pathologists, before finally reaching neurologists to be properly diagnosed.

Despite the adversities they have faced, the spirit of these individuals shone through. in the end of the call they shared their creative pursuits, including art, signifying their refusal to succumb to the challenges posed by Parkinson's, like tremor, dystonia, and dyskinesia, that commonly interfere with small movements, such as writing and drawing. Their stories serve as a poignant reminder of the resilience of the human spirit and further fuel our determination to pioneer early diagnostic solutions for Parkinson's disease, ensuring that others do not endure the prolonged uncertainty they experienced.

Medhub Meeting

Dr. Spiros Efthimiopoulos

Mr. Efthimiopoulos, a distinguished Professor at the National & Kapodistrian University of Athens, Department of Biology, specializing in the Molecular and Cellular Basis of Alzheimer's disease, was of valuable help to our project. We sought his expertise due to his extensive research background, including studies on Alzheimer's disease mechanisms and his status as a founding member of the Hellenic Society for Neurosciences.

In our initial meeting on 12/02, we discussed at the early stages of our idea for a Parkinson's disease diagnostic test. Mr. Efthimiopoulos confirmed that it was a worthy concept, pointing out its significance as a tool accessible to the general population, unlike exams like DAT SCAN.

During our meeting on 20/02, Mr. Efthimiopoulos facilitated our introduction with experts in the field of Parkinson's disease. We discussed our project’s idea, reviewed relevant research papers, and explored the possibility of using a cell-free system. While assuring us of the value of our idea, he, along with the experts, advised against the cell-free system due to its high production costs and maintenance challenges.

In our last meeting on 30/09, Mr. Efthimiopoulos continued to offer valuable insight. He guided us in how we could make sure of the connection between our phage and Volatile Organic Compounds (VOCs) while he recommended research papers on chemical modification and binding of VOCs with the phage. Although some of the proposed ideas proved too complex for our project, his feedback and recommendations contributed to refining our approach.

Overall, Mr. Efthimiopoulos served as an invaluable resource, sharing his expertise and perspective at critical points in our project's development. His contributions helped us find a more practical and effective solution for Parkinson's disease diagnosis.

Dr. Leonidas Stefanis

Katerina Margariti

Ioannis Paspaltsis

Dr. Aristides Eliopoulos

Maria Gazouli

Nefeli Lagopati, Orsalia Hazapis, Katerina Polyzou

Dr. Nikolaos Kourkoumelis

Katerina Papathoma

Alexia Polissidis

Dr. Nadeem Kasmani

Efstratios Stratikos

Smyrnaki Eugenia and Laskaridis Emmanouil

Education

Our journey all these months in organizing educational events has been proof of our vision to make science accessible and comprehensible for everyone, regardless of their age or background. Each event was not just an opportunity to spread knowledge but a platform to spark curiosity and foster constructive dialogue.

From Science Game Night to the screening of Gattaca, and from the Biosafety debate to the National Competition of Riddles in Synthetic Biology, our goal has always been the same: to break down barriers that often surround complex scientific subjects. We wanted to demonstrate that science can be fun, interactive, and easily understood by all.

In every event, our focus remained on promoting dialogue, encouraging people with diverse levels of knowledge to participate in conversations about biology, synthetic biology, and topics like Parkinson's disease, which was always the core of our project. We wished to contribute to the sharing of the wonders of science with open hearts and open minds, as we believe that a shared understanding of science can lead to a brighter, more informed future for all.

Webinar with the students of the "Evangelliki School"

Our very first Human Practice was a webinar, where our team had the privilege of engaging with the bright students of an esteemed school in Greece, the famous Evangelliki School.

At the beginning, a member from our previous iGEM team, Marianna Mitsioni, who was once a student at this very school, warmly introduced us. Her success story in the iGEM 2022 competition, where her team won a gold medal, served as an inspiring prologue for the online meeting. Understanding that synthetic biology is a complex project, we explained its basic concepts. Through an interactive presentation and engaging visuals, we introduced the students to this exciting field, inviting them to explore further. Along with Marianna, we later described the journey of the previous iGEM Athens project, "PERSPECTIVES", recounting its innovative basis and real-world applications. The students were very interested as we shared how "PERSPECTIVES" made a tangible impact in the world. We also discussed some new project ideas for the upcoming iGEM competition, demonstrating the endless possibilities in the field of synthetic biology. Transitioning from science to personal journeys, we spoke of our college experiences. We revealed our diverse academic backgrounds and how our chosen fields of study uniquely contribute to our comprehension of synthetic biology. Lastly, we shared our dreams and aspirations, talking about the future that we envision for ourselves. We discussed potential career paths, the industries we hope to impact, and the meaningful roles we aim to play in the advancement of science.

The webinar was an enriching exchange of knowledge and dreams serving as a bridge between the world of iGEM and the potential future scientists in that virtual room.

NGO's Fair

We participated in an event on the 21st of March, hosted in the National Technical University of Athens, called NGO's Fair, along with other fellow students and student teams, where we presented several exciting things about synthetic biology, its applications, and why it is useful. The NGOs' Fair is an initiative of the student organizations aiming for students to get to know the volunteer groups that are mainly active in the NTUA, to learn about the opportunities they offer through them, but also to be better informed about their activities.

More specifically, we presented in a room full of students and professors of the National Technical University of Athens, to people who had no prior knowledge of the subject, some basics of molecular and synthetic biology and its applications in everyday life. We also set up an interactive display showcasing some of the synthetic biology projects our team has worked on. We included posters, models, and videos that explained how the projects were designed and how they function. After the presentation, we had our own bench, where we met with interested students and other attendees. We had short talks on different topics related to synthetic biology that covered topics such as the history of the field, current research trends, or even ethical considerations. We also talked with students individually to explain why they should join the team, regardless of their educational background. We explained that synthetic biology is a highly interdisciplinary field that welcomes students from a wide range of backgrounds, including biology, engineering, computer science, and more. We emphasized the many benefits of joining a research team, including hands-on experience, networking opportunities, and the chance to make a real impact on the world. Finally, we used social media to promote our team and the fair, and to connect with potential new members. We shared information about our team's project and activities, as well as photos and videos from the fair itself. We also invited the audience to fill out a questionnaire, in order to obtain valuable feedback for our continuous improvement.

During the NGO's Fair, our team not only had the opportunity to educate students and professors about synthetic biology but also gained valuable insights into communicating complex scientific concepts to a general audience. We contributed by fostering interest in interdisciplinary collaboration and research, encouraging students to join our team and explore the world of synthetic biology, while also getting feedback to improve our outreach efforts and engagement with the community.

Entrepreneurship Fair Panorama

UNIque Days by Unique Minds

In early May, our team had the honor of participating in the "UNIque Days" event, organized by UNIque Minds at the National Technical University of Athens. The event welcomed students from junior high schools and high schools, offering a valuable platform to explore the fields of science and engineering.

Throughout the day, we described our respective educational backgrounds, underlining the diversity of academic origins that came together to form our team. We emphasized that our collective journey into the intricate world of synthetic biology and participation in the prestigious iGEM Competition had its roots in a shared scientific curiosity. We wanted to show our young audience that this same curiosity could be the catalyst for their own future. We presented our iGEM team's work, showcasing the innovative projects the team has prepared and the real-world impact they have the potential to achieve. We pointed out the pivotal role engineers play within our team, since there are both chemical and electrical engineers, elucidating how their diverse skill sets, spanning from software development to design work, significantly help solve problems that we face. This underscored the message that there are many entry points into the world of synthetic biology. To inspire these potential scientists, we advised the students regarding the selection of an appropriate academic path. We encouraged them to align their academic pursuits with their own passions, whether that be biology, chemistry, engineering, or other disciplines.

Participating in the "UNIque Days" event allowed our team to inspire young minds with the possibilities of synthetic biology and interdisciplinary collaboration. We contributed by emphasizing the diverse backgrounds and skill sets within our team, demonstrating that scientific curiosity can lead to new solutions, and offering guidance on academic paths, ultimately fostering an interest in science and engineering among the students.

Junior High School in Xanthi

Our team embarked on a mission in April to ignite scientific interest in a junior high school classroom, situated in the distant Xanthi, in northern Greece.

There, we met the teachers of Xenia, one of our members who was raised there, and we commenced by defining the foundational stones of biological knowledge. From nucleotides to the Central Dogma of Biology, we explained the intricacies that form life itself. The hands-on DNA banana extraction experiment that we performed together served as a bridge between theory and practice, which helped them understand everything that we described before. As we transitioned into the topic of synthetic biology, we discussed the boundless possibilities it offers, through an engaging presentation and a clapping game. The students were presented with several concepts of synthetic biology that have already been constructed or have the possibility of being created and others that are imaginary. They clapped everytime that they thought the idea was feasible. For example, we described the existence of new genetic material, apart from DNA and RNA, or genetically modified algae that produces proteins.

Our journey to Xanthi was a testament to the transformative power of knowledge and the profound impact that the iGEM community can have in nurturing young minds, regardless of geographical distances, as we helped the students broaden their horizons, inspiring them to explore the exciting possibilities in the world of science and synthetic biology.

Panhellenic Symposium of College Students for Bioscientists

From the 28th to the 30th of April 2023, our team had the privilege of participating in the 1st Panhellenic Symposium of College Students for Bioscientists, hosted by the college students of the Molecular Biology and Genetics school.

Throughout the symposium, we attended a series of remarkable talks and speeches that ranged from currently relevant themes such as Sustainability, Biodiversity, Climate Change to always relevant topics such as Epigenetics, Cellular Ageing, Cancer, and the intricacies of Psychiatric and Neurological Disorders. By participating in the "Modeling in Synthetic Biology" workshop, we learnt how to convert qualitative to quantitative results, gaining valuable insight into this dynamic field. On the second day, we took the stage before an audience of college students and esteemed professors. There, we meticulously described our project, emphasizing the complexities of Parkinson's disease, its origins, symptoms, and available treatments. We explained the phage technology that we are using in our test kit, detailing our modification strategies and the development of an accompanying mobile application for our diagnostic kit. In the end of the presentation, we answered challenging questions from the audience regarding the feasibility and methodology of our project.

During the Symposium, our team gained valuable insights from the diverse array of talks and speeches, deepening our understanding of various topics in the biosciences. When presenting our project on Parkinson's disease and its diagnostic kit, we shared our in-depth knowledge and innovative solutions with fellow college students and professors. By addressing people's queries, we not only proved the potential of synthetic biology but also had meaningful discussions and exchanged ideas, enriching the symposium's intellectual environment.

Scientific Congress of Medical Students of Greece

We participated in the 28th Scientific Congress of Medical Students of Greece, held from May 5th to May 7th in Alexandroupolis. Within the conference, students were given the opportunity to develop scientific and research work that is in line with their interests, to get familiar with the preparation and presentation of scientific papers, to get in touch with renowned professors, but also to meet fellow students from all over Greece and abroad.

During our collaboration with iGEM Thessaly and iGEM Thrace for the poster presentation, we gained insight into the diverse applications of Synthetic Biology in medicine and public health. Our focus on genetically engineered E.coli, used to metabolize phenylalanine, for the treatment of phenylketonuria (PKU) showed the potential of synthetic biology to address critical medical issues. Interacting with medical students from various regions of Greece allowed us to broaden our perspective and share our projects with a broader audience. This exchange of ideas and knowledge was mutually beneficial, as we learned about their interests and concerns related to medical applications of synthetic biology. Two of our members were also instructors in two workshops on Biomedical Engineering, while one presented on the Neurology round table.

Attending presentations on Parkinson's disease specifically strengthened our knowledge of the challenges and opportunities in this field, aligning with our project's focus. Overall, this collaborative event increased our appreciation of the medical field and the significance of our work in contributing to the well-being of individuals through synthetic biology solutions. Our team members were exposed even more to the field of Medicine, giving us a more detailed view on our project's most important stakeholder: The human body.

Pharmaceutical Conference of Pharmaceutical College Students

"SKAI" Radio

During the summer we were honored with not one, but two invitations to feature on the primetime radio shows of Greece's largest and most renowned station, SKAI.

These radio appearances, witnessed by thousands of eager listeners, provided us with a unique platform to amplify the essence of our iGEM team's mission. In the spotlight, we highlighted the multidisciplinary nature of our team, the iGEM Competition, and our groundbreaking project focused on early Parkinson's disease diagnosis. We raised awareness about the insidious nature of Parkinson's, delving into its cause, symptoms and treatment, and of course our test kit, how it works and its profound impact that our diagnostic test that it promises, enhancing the quality of life for potential patients. Beyond our scientific pursuits, we talked of our academic journeys, aspirations for the future, and our thoughts to remain in Greece or venture abroad to contribute to the global scientific community. We pointed out the challenges and hurdles in greek laboratories, thanked our mentors for their invaluable support, and shared the resourceful means through which we secure funding for our project.

Our appearances on SKAI's primetime radio shows provided us with an exceptional opportunity to inform the listeners about Parkinson's disease and our diagnostic test kit, and at the same time, we shared our experiences, talking of the challenges faced by students pursuing scientific careers in Greece and offering inspiration and encouragement to aspiring scientists and researchers.

Educational Presentation & Workshop in "Metadrasi"

On the 13th of September, our team embarked on a mission to "Metadrasi", an association with the mission of facilitating the reception and integration of refugees and migrants in Greece. In their educational center we met young kids aged 7-14 who have endured hardships in war-torn regions.

Our educational journey began with an introduction to fundamental biology concepts. Through engaging animated educational videos, we spoke of nucleotides and the central dogma of biology, explaining processes such as replication, transcription, and translation. The highlight of our workshop was a hands-on DNA banana extraction experiment, where all the kids participated happily. Witnessing the extraction of DNA firsthand, they gained deeper knowledge of the genetic material and how it looks and works. Later, we delved into the topic of germs, describing their origins, growth, and survival techniques. To make these concepts tangible, we presented photos of Petri dishes. We had taken samples of items that we use everyday, like our laptops, our phones, the lab's fridge and then we left them to grow in Petri dishes with a growth medium, and then we took photos of the dishes. Thus, they understood much better how bacteria and fungi grow and how they are found in every space possible. Lastly, we introduced the concept of synthetic biology, demonstrating how bacteria can serve as tools for innovation and scientific problem-solving.

We provided them with hands-on experiences and knowledge about biology and synthetic biology, fostering their interest in the subject and hopefully igniting a spark of curiosity that can guide them toward a brighter future. For our team, this event was a humbling experience, reminding us of the power of education to transcend boundaries and offer hope and inspiration even in the most difficult of circumstances.

Science Game Night

On the 15th of September, we hosted an event that combined fun and education: the Science Game Night, where we brought together friends, family, and our social media followers, of all ages and educational backgrounds.The event was held in PLAyCE, a famous Athenian cafeteria with boarding games, with free entrance, so every fan of biology could attend!

Our evening commenced with a brief PowerPoint presentation, offering an introduction on the fundamentals of biology, including cellular biology, zoology and phytology. More specifically, we spoke of the intricacies of cellular structures and functions, but also of the basic information about mammals and photosynthesis. Subsequently, we discussed Parkinson's disease, explaining its causes, symptoms, and treatment options. Then we started a lively and engaging quiz featuring 40 multiple-choice questions. The questions spanned a wide spectrum, including cellular biology, general knowledge in animals, human organ systems, and the information we had just shared about Parkinson's disease. Participants formed teams, collaborating to find the correct answers. The competitive spirit was high as teams vied for the coveted prize, free tickets for PLAyCE, making the quiz both educational and entertaining. Following the quiz, we introduced customized and science-themed adaptations of popular games such as TABOO, Pictionary, and Pantomime cards. These games, which turned out to be quite the hit, were thoughtfully designed by us to incorporate biology and scientific concepts, fostering an atmosphere where learning merged with enjoyment.

Science Game Night succeeded not only in sparking curiosity but also in demonstrating that science can be both intellectually stimulating and incredibly fun. It was a delightful occasion, reaffirming our commitment to making science accessible and enjoyable for all. Apart from sharing our knowledge of biology and Parkinson's disease in a fun and interactive way, thus helping to make science more accessible and engaging for those who attended, we also gained the satisfaction of seeing participants of all ages actively engage with scientific concepts and enjoy themselves while learning, reinforcing our mission of promoting science education and outreach in an entertaining manner.

Presentation in "Sivilla"

On the morning of the 16th of September, we had a presentation at the Sivilla Home for the Elderly, located in southern Athens. It was an enriching experience where we attempted to ignite the spark of curiosity among the residents and share valuable knowledge.

We started the event by showing a PowerPoint presentation about the basis of life - DNA. We simplified this complex concept, illustrating it as the recipe that guides all living organisms. The highlight of the session was the hands-on DNA extraction experiment using bananas, making the seemingly abstract world of genetics tangible. Right after, we introduced the residents to the applications of synthetic biology that have the potential to transform healthcare, including theirs. While we acknowledge that not all of them might directly benefit or use this information, we aimed to instill hope in the possibilities of a brighter future because of the potential innovations of synthetic biology. The importance of hygiene and cleanliness was later a central theme as we discussed the microscopic world of germs, including fungi, bacteria, and viruses. We shared images of cultivated petri dishes, each containing samples from common everyday items, showing the residents about the unseen threats in their surroundings. Turning our attention at the end of the presentation to Parkinson's disease, we delved into its causes, symptoms, and treatment options. We emphasized the vital role of early detection and how our test kit could significantly impact the lives of individuals by facilitating quick and accurate diagnosis. As our session concluded, we engaged in heartfelt conversations about the residents' lives, and experiences, making it a memorable and touching day of sharing, learning, and making connections at Sivilla.

Our team left the Home with a profound sense of fulfillment, as we had the opportunity to share our knowledge with the residents. It was a deeply rewarding experience, and we felt a strong connection with the elderly residents, since they reminded us of our own grandparents. Simultaneously, we felt that we helped them by providing them with information about hygiene, and the potential benefits of synthetic biology, aiming to inspire hope for a brighter future through scientific advancements and early disease detection, while at the same time we 'shook things up' in Sivilla with our laughs!

Biosafety Presentation-Debate and Theatrical Games-Improvisation

On the 27th of September, our team addressed the crucial topic of biosafety, hosting an event at the University of West Attica. Biosafety measures are at the core of responsible scientific research, and it was our mission to point out their significance.

During the first hours, we had an extended, yet interesting biosafety presentation.The Vice-Chanchellor of the University, Dr Theodoros Xanthos, who was present at the event, also talked of his experience of being a cardiologist and theatrologist, and how theater is being used as a teaching method in medical environments. We explained why biosafety measures are of the highest importance within laboratory environments, delving into the specifics of where these measures are applied and the many safety protocols enacted in laboratories. We analyzed the levels of biosafety present in various lab settings and elaborated on the significance of the biosafety signs that we all see in our labs. A debate followed, featuring members of the previous iGEM Athens team from 2022, who had their own debate last year, thus we upheld the tradition by taking a deeper dive into the topic: "Are Biosafety Regulations Excessive?" The debate revolved around the question of whether contemporary biosafety measures are excessive or justified. In a calm, yet highly interesting exchange of ideas, the team in favor of tight biosafety measures won. Evidently, all participants and speakers endorse biosafety regulations, making it an intriguing challenge to employ theatrical methods to advocate for a stance they don't genuinely uphold.

The following hours, we made good use of this knowledge into an interactive biosafety improvisation workshop. Through theatrical games and immersive scenarios, we along with the audience reenacted potential biosafety mishaps, such as the accidental spillage of a petri dish containing hazardous viruses. A notable example was the rapid comprehension of fundamental biosafety rules, particularly among those who had no prior exposure to the concept, as illustrated in the diagrams. By acting out these scenarios, we not only reinforced our understanding of proper biosafety procedures but also honed our theatrical skills, making learning an enjoyable and funny experience.

Our team gained a deeper understanding of the importance of biosafety measures and enhanced our knowledge of how to handle potential biosafety mishaps through the interactive workshop. We also improved our communication and teamwork skills through the debate and theatrical scenarios. The audience we believe greatly benefited from the event by learning of the significance of biosafety in laboratory environments. They grasped the different levels of biosafety and gained a practical understanding of how to respond to biosafety challenges through the interactive scenarios. Functioning as a workshop, we prioritized the teaching of scientific subjects through theatrical techniques. This was affirmed by participants in the questionnaire, with unanimous support for theater as an effective educational method.

You can find indicative theatrical scenarios and other games that we prepared for the event, all to educate people about Biosafety through theater, analytically in the Contributions section of the Wiki.

Medhub Meeting

On the 28th of September at the Athens Medical School, members of our team met with MedHub, a dedicated group of students from the Athens Medical School committed to advancing biomedical engineering knowledge and applications.

The meeting began with MedHub presenting their ongoing projects, affording us a comprehensive glimpse into the intricate realm of biomedical engineering. More specifically, they focused on "Symmyetry", a circuit that transfers a muscular contraction from one person to another, using an Arduino and a TENS machine, resulting in somebody mimicking another person's movement "against their will". They explained the technologies and methodologies behind their initiatives, enabling us to gain a profound appreciation for their work. In reciprocation, we shared the details of our diagnostic test kit for Parkinson's Disease. As we delved into the difficulties we face in our project, the MedHub team offered invaluable input, drawing from their wealth of knowledge spanning medical and electrical engineering domains.At the end of the meeting, we mutually recognized in each other the efforts that our teams make, despite the challenges that student-driven initiatives in Greece continuously encounter, particularly in securing funding and recognition within an educational landscape that may not always easily accommodate such ideas.

Our team acquired valuable knowledge into the field of biomedical engineering and learned about the innovative projects being undertaken by MedHub. We also benefited from the exchange of ideas and the opportunity to collaborate with experts in the medical and electrical engineering domains. MedHub in return was happy to learn of our diagnostic test kit for Parkinson's Disease and the potential impact it can have on healthcare. They also had the opportunity to share their expertise and provide input into our project, fostering a collaborative environment between our teams.

Researcher's Night by the National Technical University of Athens

On the 29th of September, our team took part in the prestigious Europe-wide event known as "Researcher's Night" at the National Technical University of Athens. This event serves as a dynamic platform for celebrating the different branches of science and its profound impact on the daily lives of citizens, delivering knowledge in an engaging and approachable manner.

Our team hosted an exhibition at our designated stand, where we presented 2 scientific domains: Polymerase Chain Reaction (PCR) and the world of blood types, including the pivotal roles played by antigens and antibodies in determining blood compatibility. To offer a hands-on experience, we provided a high-powered microscope, allowing attendees to see in detail the blood cells of a healthy individual. Alongside this, we displayed images of blood cells from individuals with blood disorders like Drepanocytosis and Thalassemia, facilitating direct comparisons and better understanding. The best part of our exhibition involved demonstrating how blood typing is done. We tested several times the blood type of one of our team members using A and B antigens, captivating the audience, particularly the younger visitors, with the sight of blood clotting in response to antigen-antibody reactions. Furthermore, we showcased a PCR machine to explain the principles of this incredibly useful technology, even encouraging our audience to operate pipettes for a better understanding of the PCR process, while using plain water. Colorful paperboards were also used to represent genetically modified plasmids and visually convey the appearance of these essential genetic components. We supplemented this with vivid photographic comparisons, showing photographs of bacteria before and after plasmid transformation, emphasizing the transformative power of genetic modification. Throughout the event, we had discussions with attendees spanning various ages and educational backgrounds, reflecting the universal appeal of biology.

Our team had the opportunity to engage with a diverse audience and share our knowledge and passion for biology. Interacting with attendees allowed us to refine our communication skills and deepen our understanding of how to make complex scientific concepts accessible and simple. The audience benefited from our exhibition by gaining insight into important scientific domains like PCR and blood typing. Our hands-on demonstrations and visual aids helped explain these concepts, making them more comprehensible.

Educational Presentation & Workshop in "Smile of the Child"

On a mission to make a positive impact and contribute to the education of all children in Greece, our team recently had the rare privilege of collaborating with "The Smile of the Child," a widely admired voluntary, non-profit child welfare organization located in Athens. This organization, inspired by the heartfelt vision of young Andreas Yannopoulos, who while suffering from incurable cancer, wished to ensure that all children experienced the same love, care, and respect he had enjoyed, has grown into an internationally recognized entity. On the 30th of September, we organized two simultaneous presentations and workshops, tailored to different age groups.

For the younger group, aged 7-12, we prepared lively activities centered on fundamental biological concepts. Through animated videos, we explored the fascinating world of cells, DNA, and bacteria, making the learning experience both educational and enjoyable. The favorite part of the children was when we guided them in creating double helix DNA structures using candy—which they ate after washing their hands. Following this delicious activity, we offered the children the choice of drawing biology-related pictures, like bacteria, a human heart, eukaryotic cells, or engaging in word-based games like Pantomime, TABOO, Pictionary, all of which we had adapted to include science-related themes, like the cards what we designed for Science Game Night, but different ones, since now we had a younger age target group. At the same time, in another room, other members of our team entertained the older group, aged 12-18, addressing more advanced topics such as the central dogma of biology and the fundamentals of synthetic biology. The teenagers actively participated in the banana DNA extraction experiment, gaining a deeper understanding of scientific principles. To conclude, we introduced them to science-based games, including Pantomime, TABOO, and Pictionary. Post-event, we had the privilege of touring the organization's farm, which is right next to the educational center, providing the children with easy access to farm animals. Our extended conversation with the organization's president, Mr Yannopoulos-the father of young Andreas who has passed since the founding of the organization. He shared his profound insights into the care and respect children deserve, as well as his thoughts on the Greek educational system and its need for reform. His dedication and resilience, even in the face of personal loss, were a testament to the organization's mission and the legacy of young Andreas Yannopoulos.

During this collaborative event with "The Smile of the Child," our team had the amazing experience of sharing our passion for science with young children and teengers, fostering their curiosity, and making learning enjoyable. We also had the privilege of witnessing firsthand the incredible work of "The Smile of the Child" in providing care and support to children in need, reinforcing our commitment to making a positive impact on education and child welfare in Greece. "The Smile of the Child" we believe mutually benefited from this event by showing to the children engaging and educational science activities, broadening their horizons and igniting their interest in the field. Furthermore, the event provided an opportunity for intergenerational exchange, as the organization's president, Mr. Yannopoulos, shared his opinions on child welfare and education, enriching our understanding of the challenges and opportunities in this important sector. Overall, the event was a mutually enriching experience that strengthened our shared commitment to the well-being and education of children in Greece.

Science Movie Night: Gattaca in Open Air Cinema

On October 1st, we had the incredible opportunity of hosting a memorable event at CineAlimos, an open-air cinema in southern Athens. This free-admission movie night featured the screening of the thought-provoking film "Gattaca." To enhance the experience, we provided complimentary beverages and food, creating a convivial atmosphere.

At the beginning of the night, we presented our team and our project, even showing to the audience our Promotion Video. Then, we spoke of the film and why we chose this specific one on our movie night. We pointed out that "Gattaca" is a prophetic movie, even though it came out 25 years ago, sadly performing poorly in the international box office, serving in 1997 as an introduction for many to groundbreaking concepts such as genetic modification. We pointed out the predictive nature of the film, as it foreshadowed the utilization of the depicted techniques in contemporary society, some of which are used by us currently at the lab. Moreover, we emphasized our commitment to using genetics for positive purposes, in stark contrast to the film's cautionary narrative. Our conversations touched the topic of bioethics, a usual controversy in genetics.This platform allowed us to make plain our dedication to ethical and responsible scientific practices. Notably, the event featured a special guest: the President of the Parkinson Athens Club, Madame Foteini Skondra. Her presence attached great importance to the occasion as she addressed the audience, acknowledging the vital work of our team and the impact we aim to have on the community. We were deeply honored by her participation. Following the film screening, we engaged in post-movie discussions with the audience, encouraging them to share their thoughts and reflections on "Gattaca." In summary, the evening at CineAlimos was a testament to the power of film and discussion in exploring the intersection of science, ethics, and society.

This event at CineAlimos provided our team with a unique platform to bridge the gap between science and society. We gained the opportunity to not only show our dedication to responsible genetics, but also to engage with the community and foster a deeper understanding of the ethical dimensions of scientific advancements. For the audience, we offered a thought-provoking cinematic experience paired with enlightening discussions, encouraging them to contemplate the implications of genetic technologies. We aimed to activate critical thinking and promote a responsible approach to science, thereby contributing to a more informed and ethically conscious society.

Bioinformatics Workshop

National Competition of Riddles in Synthetic Biology

Organizing Greece's first-ever online National Competition of Riddles in Synthetic Biology on the 5th of October was a pioneering initiative for our team, and we were thrilled to witness the enthusiastic response from numerous high schools. There was active participation of hundreds of students hailing from various regions across the country, encompassing the capital, rural areas, and islands alike, including also those with whom we had previously collaborated in various educational events.

On July 30th, we submitted our application form, complete with all the required documents, to seek official approval from both the Education Policy Institute (ΙΕΠ) and the Ministry of Education for our competition.In our dedicated effort to plan the competition effectively, we developed valuable partnerships and maintained open channels of communication with the Panhellenic Union of Bioscientists (ΠΕΒ), the organizers of the National Biology Competition that culminates in the prestigious International Biology Olympiad (IBO).

It was held in one phase, on Thursday, October 05, 2023 at 9.30 a.m. It will be conducted at the High Schools with an initial online briefing of the students on the iGEM team and Synthetic Biology and the presentation of further information required for the puzzles, lasting one hour, followed by a fifteen minute break, and then the competition that was conducted after, lasted less than two hours.

At the start of the day, we began with an hour-long presentation that showed the fundamentals of synthetic biology and delved into the fascinating realm of combinatorics.The riddles were made for the knowledge the children already possessed from their junior high school education. Not only did we refresh their existing knowledge with a subtle reminder of what they already knew, but we provided them with additional, more specialized insights in biology and mathematics that were essential for them in order to effectively play with the challenging problem-solving puzzles. After our team’s member Filoxeni's informative presentation on biology, another member, Dimitris proceeded to delve into the subject of combinatorics. He may be studying Medicine but is passionate about Mathematics, a branch of mathematics that explores counting, arranging, and organizing objects in various ways. The presentation which you will find below the riddles involves the study of permutations, combinations, and the systematic arrangement of elements, all of which are crucial in the field of synthetic biology for designing and solving complex genetic puzzles.

Following the educational presentation, we sent via email to the participating schools six complex yet very interesting problems - riddles that needed knowledge of synthetic biology concepts with combinatorics, all of which we had explained right before. The students showed remarkable problem-solving skills, facing the riddles and in the end proving their understanding of both subjects. During the presentation and conduct of the competition, there was a form for questions and queries that arised. Schools were contacted directly, relaying questions from students.

Always respecting the GDPR regulations, we have received the following photographs from some of the schools that participated in the contest, and we have chosen to display a selection of them.

For our team, this event was an opportunity to develop interest for synthetic biology and mathematics in young minds while nurturing their critical thinking abilities. Seeing the students' excitement and the positive feedback from their teachers reaffirmed the value of such initiatives. We needed this encouragement, since it was very hard to organize this event on such an extended scale. Our persistence though proved fruitful, as all congratulated us on our efforts and wished to participate again next year.

The children were introduced to a diverse range of subjects, including planarians and techniques for genetic modification. In the subsequent presentations, they not only gained exposure but also actively engaged with the concept of synthetic biology. This exposure served as a catalyst, fostering an understanding that synthetic biology is an interdisciplinary field with exciting career prospects that they could explore in the future. The objectives of the competition are to inform the team, to familiarize them with research and scientific competitions, as well as to gain knowledge useful for their later career as potential scientists and future members of teams like iGEM Athens. We were delighted to contribute to the students’ education, challenging them to explore the fascinating meeting points of science and mathematics, and we hope that these experiences will inspire their future academic choices.We extended our support by recognizing their efforts and achievements, rewarding them with commendations and certificates of participation, tailored to their individual performances. In return, their enthusiasm and determination served as a reminder of the boundless potential that education has in shaping the next generation of scientists and problem solvers.

Educational Presentation & Workshop in "SOS Children's Villages"

On the 6th of October, our team organized an educational presentation and workshop for the adolescents of 'SOS Children's Villages,' with participants approximately 14-16 years old. 'SOS Children's Villages' is an esteemed international non-governmental organization dedicated to ensuring the well-being of children worldwide. The organization operates in 136 countries and territories, impacting the lives of countless children annually. Their commitment to providing alternative care, family strengthening, educational opportunities, healthcare access, and community support aligns closely with our own mission to nurture young minds and foster a love for science.

There, we had a presentation with the fundamentals of biology, defining key concepts such as the central dogma and the wonder of DNA. To pass from theory to practice, we performed with the kids a captivating experiment - the extraction of DNA from strawberries, deeply understanding genetics in a hands-on and enjoyable manner. Furthermore, we shared images of petri dishes with colonies of bacteria that grow on everyday objects, initiating a conversation about germs, their growth patterns, and the essential measures of hygiene to protect us against them. We also explained what blood types are and how they work, highlighting the crucial roles of antigens and antibodies in establishing blood compatibility. To create a more interactive learning experience, we showed images of blood cells from individuals with conditions such as Drepanocytosis and Thalassemia, facilitating direct comparisons and improving comprehension. The most captivating aspect of our exhibition was our live demonstration of blood typing. We conducted multiple tests to determine the blood type of one of our team members using A and B antigens, captivating the audience, especially the younger attendees, as they witnessed blood clotting in response to antigen-antibody reactions.

Our collaboration with 'SOS Children's Villages' strongly proves our collective dedication to championing the welfare of all children. We stand united in our efforts to raise awareness about child abuse and neglect, advocating tirelessly for enhanced protection for every child, regardless of their circumstances.

DNA Workshop "DNA Delights" in the Athens Science Festival

"Smile of the Child" - Biology Workshop

Education through our Social Media

Educational Series of Videos: Introduction to Combinatorics

Our team decided to create an educational series of videos as an introductory step to Combinatorics.

Combinatorics is an enormous mathematical subject that has numerous implementations on Synthetic Biology, as well as other fields. It teaches us how to think strategically, optimize our decisions, and improves our problem-solving technique. In this series, the viewer will learn all the combinatorial formulas required to solve basic problems in combinatorial analysis. Every concept is analyzed in depth and as clearly as possible, so that no mathematical knowledge background is required to watch the videos. All the examples come from the world of synthetic biology, to demonstrate the vast amount of implementations that Combinatorics have. There is no age target group for these videos, anyone can watch them and we hope that they will be able to understand everything. In fact, this is the magic of combinatorics: it has a very limited amount of theory, the problem statement is easily understandable, however the problem itself may get extremely challenging. We hope these videos inspire the viewers to study this wonderful subject more, and to implement mathematical thinking into their field of science, no matter what that is!

In our educational video series on Combinatorics, we taught viewers the fundamental concepts and formulas needed to solve combinatorial problems, exploring how combinatorics is applied in various fields, especially in Synthetic Biology. More specifically the videos regard:
1. Introduction & The Multiplication Principle
2. Factorials and Permutations
3. Combinations
4. Revision & Exercises
5. A combinatorial problem
6. Probability
7. The Inclusion - Exclusion Principle
8. The Binomial Theorem & Pascal's Triangle
We hope that these videos inspire viewers to delve deeper into this subject and apply mathematical thinking to their own areas of interest, regardless of their age or mathematical background.

Here are some highlights from the videos, which are uploaded on YouTube platform.

https://www.youtube.com/@iGEMAthens2023