Utilized and Characterized Existing Parts
| Description | Part name | Description |
| SQR | BBa_K3823001 | SQR is a sulfide:quinone oxidoreductase from Acidithiobacillus thiooxidans, which can oxidize H2S to elemental sulfur (S0) and transfer electrons to quinone. |
| SDO | BBa_K3823002 | SDO is a sulfur dioxygenase from Aquifex aeolicus, which can convert S0 to sulfite (SO32-) using molecular oxygen. |
| AprBA | BBa_K3823003 | AprBA can reduce APS to sulfite and AMP in sulfate-reducing bacteria. |
| SAT | BBa_K3823004 | SAT (ATP sulfurylase) is from Acidithiobacillus spp, which can synthesize APS from sulfite and ATP. |
| FMO | BBa_K1131000 | Flavin-containing monooxygenase (FMO) from M. aminisulfidivorans. |
| QsrR | BBa_K1092000 | QsrR is a transcriptional repressor from Staphylococcus aureus, which can sense and respond to quinone stress by regulating the expression of genes involved in quinone detoxification. |
| Dioxygenase(catA) | BBa_K1092002 | catA is a catalase from E.coli , which can decompose hydrogen peroxide, a byproduct of cotA activity, to water and oxygen. |
| Laccase(cotA) | BBa_K1092004 | cotA is a multicopper oxidase from Bacillus pumilus WH4, which can oxidize benzo[a]pyrene to quinones and hydroquinones. |
New Basic Parts
| Description | Part name | Function |
| RBS-NicA | BBa_K4773000 | NicA can encode the enzyme which may degrade nicotine to SP. |
| RBS-NicB | BBa_K4773001 | NicB catalyzes the hydrolysis of SP to 6-hydroxy-3-succinoyl pyridine. |
| RBS-NicC | BBa_K4773002 | NicC encodes transcriptional regulator; similar to Pseudomonas putida KT2440 transcriptional regulator AsnC and to Psuedomonas putida F1 helix-turn-helix protein. |
| RBS-hps | BBa_K4773003 | This part is a sequence containing the hps gene, which encodes 3-hexulose-6-phosphate synthase (HPS). |
| RBS-phi | BBa_K4773004 | Phi is responsible for degrading H6P to fructose-6-phosphate (F6P). |
| RBS-QsrR | BBa_K4773005 | QsrR is a transcriptional repressor from Staphylococcus aureus, which can sense and respond to quinone stress by regulating the expression of genes involved in quinone detoxification. |
| RBS-ycnE | BBa_K4773006 | ycnE can degrade indole to isatin. |
| RBS-buk | BBa_K4773007 | Degradation of butyric acid to butyrophosphoric acid. |
| RBS-ptb | BBa_K4773008 | Degradation of butyrophosphate to butyryl coenzyme A. |
| RBS-adhE2 | BBa_K4773009 | Degradation of butyryl coenzyme A to butanol. |
| RBS-ATF1 | BBa_K4773010 | ATF1 synthesizes acetyl butyl ester. |
| RBS-HAO | BBa_K4773017 | HAO is a hydroxylamine oxidoreductase from Nitrosomonas europaea, which can oxidize hydroxylamine (NH2OH). |
| RBS-HmpA | BBa_K4773018 | HmpA is a flavohemoglobin from E.coli, which can reduce nitric oxide to nitrate using NADH and oxygen. |
Composite Parts
| Part name | Part number | Function |
| Lac Promoter+RBS+NicA+RBS+NicB+RBS+NicC+Term | BBa_K4773011 | This part enables the degradation of nicotine. |
| Lac Promoter+RBS+SQR+RBS+SDO+RBS+AprBA+RBS+SAT+Term | BBa_K4773012 | This part is a pathway combination consisting of SQR, SDO, AprBA, and SAT, which can degrade hydrogen sulfide (H2S). |
| J23100 Promoter+RBS+ycnE+RBS+FMO+Term | BBa_K4773013 | This part is a gene combination consisting of ycnE and FMO, which can degrade indole, a heterocyclic aromatic compound, to different colored pigments. |
| J23100 Promoter+RBS+hps+RBS+phi+Term | BBa_K4773014 | This part is a gene combination consisting of hps and phi, which can degrade formaldehyde, a toxic and carcinogenic compound, to formate and hydrogen peroxide. |
| Lac Promoter+RBS+cotA+RBS+QsrR+RBS+catA+Term | BBa_K4773015 | This part is a gene combination consisting of cotA, QsrR, and catA, which can degrade benzo[a]pyrene, a carcinogenic polycyclic aromatic hydrocarbon, to less toxic compounds. |
| Lac Promoter+RBS+ptb+RBS+buk+RBS+AprBA+RBS+ATF1+Term | BBa_K4773016 | This part is a pathway combination consisting of ptb, buk, AprBA, and ATF1, which can degrade butyrate, a four-carbon fatty acid, to sulfite and acetyl-CoA. |
| Lac Promoter +RBS+HAO+RBS+HmpA+Term. | BBa_K4773019 | This part is a pathway combination consisting of HAO and HmpA, which can degrade ammonia (NH3). |
