Safety

Every member of our team has been trained about general lab safety and project design safety by our previous members. For example, the former leader and our advisors taught us how to run the experiment correctly and avoid mistakes.

In order to prevent the bacteria from causing unknown damage in the body for a long time, we introduced an arabinose-regulated suicide switch downstream of the gene. araC is a control protein, and pBAD is a regulator promoter. In order to ensure the efficiency of arabinose uptake, we also introduced the araE protein, which is a transporter that can transport arabinose from outside the membrane to the inside of the membrane, thereby regulating the expression of ccdB.It achieves the suicide effect of bacteria, which ensures the safety of treatment.At the same time, when testing some promoter effects, we use fluorescent proteins for quantification, which are harmless to humans and can be directly observed under specific conditions.

Laboratory safety is an important aspect of protecting laboratory workers, the laboratory environment, and public safety. We have not used strains outside the whitelist in our laboratory, and all strains are safe and controllable. Here are some key principles and practices for ensuring laboratory safety: See more in Notebook.

• 1.Training and Education: All laboratory personnel should receive appropriate training and education to understand laboratory safety rules and procedures. This includes training in using laboratory equipment, handling hazardous chemicals, handling emergency situations, and more.
• 2. Safety equipment and equipment: The laboratory should be equipped with necessary safety equipment, such as safety cabinets, glasses, gloves, laboratory coats, etc. Workers should wear and use these devices correctly to reduce the risk of injury.
• 3. Chemical management: The laboratory should have a clear chemical management policy, including regulations for the storage, labeling and handling of hazardous chemicals. This helps reduce the risk of chemical accidents.
• 4. Laboratory rules and regulations: The laboratory should establish clear rules and regulations, including regulations for entering the laboratory, laboratory closing procedures, laboratory safety inspections, etc. Staff are expected to comply with these rules and regulations.
• 5. Fire safety: The laboratory should be equipped with fire extinguishers and fire alarm equipment, and fire safety rules should be strictly followed. Smoking is prohibited in the laboratory, and passages should be kept clear to ensure rapid evacuation in the event of a fire.
• 6. Personal protection: Workers should always wear personal protective equipment correctly, such as lab coats, goggles, gloves, etc. In addition, you should avoid eating, drinking, or putting on makeup in the laboratory to reduce the risk of contamination of your mouth, nose, and eyes with harmful substances.
• 7. Emergency response: The laboratory should develop an emergency response plan, including first aid procedures, accident reporting procedures, and emergency contact lists. Staff should be familiar with these procedures to respond to emergencies.
• 8. Waste Disposal: Waste should be properly handled and disposed of to ensure no pollution to the environment. Laboratories should comply with waste management regulations and use appropriate containers for the storage and disposal of waste.
• 9. Regular inspection and maintenance: Laboratory equipment and safety equipment should be inspected and maintained regularly to ensure their normal operation. This prevents safety issues caused by equipment failure.
• 10. Documentation: The laboratory should properly record all experiments, safety inspections, and accident reports. These documents can be used to track and improve measures to improve laboratory safety.

Laboratory safety is an important task that requires the joint efforts of laboratory managers and staff to ensure that work in the laboratory is safe and hazards are effectively controlled.

All dangerous chemicals was allowed only when our experimental instructor on the spot,Otherwise it's forbidened.

We must keep Laboratory tidy!!!

Chemical Management: Manage chemicals and solvents to ensure they are stored in the appropriate places and regularly inspect and clean chemical cabinets, shelves, and storage areas. Chemicals that have expired or are no longer needed should be handled and disposed of safely.

Proper placement of microbiological culture dishes is an important step in ensuring laboratory hygiene and avoiding cross-contamination. Here are some basic principles regarding the placement of microbiological Petri dishes: 1. Separate areas: Separate different types of microbial culture dishes (such as different strains or experiments) to prevent cross-contamination. Dividing different work areas within the laboratory, each for a specific type of petri dish, can help reduce the risk of contamination. 2. Avoid cross-contamination: Use different work areas or different laboratory equipment (including microbial safety cabinets, incubators) to handle different microbial culture dishes. Avoid placing Petri dishes from different sources in the same work area or on the same laboratory equipment to reduce the possibility of cross-contamination. 3. Label the petri dishes: Clearly label the strain, date and experimenter’s information on each petri dish. This helps trace the origin of the petri dish and prevent mix-ups. 4. Proper storage: Place microbial petri dishes in appropriate storage conditions, such as incubators, culture rooms, or refrigerators, to ensure the growth and preservation of microorganisms. Each microorganism may require different growth conditions, so ensuring a suitable storage environment is critical. 5. Avoid cross-contamination of mold and bacteria: Mold petri dishes and bacterial culture dishes should be stored separately to prevent mold spores from spreading to bacterial culture dishes.

Regularly clean and disinfect work areas: Regularly clean and disinfect work areas, including incubators, culture rooms, experimental benches, etc., to reduce the risk of microbial residues.

Laboratory hygiene maintenance is an essential element to ensure safe and efficient laboratory operations. Adhering to these hygiene maintenance principles helps prevent accidents, protect the health of workers, and ensure the accuracy and reproducibility of laboratory research.