Best Part Collection

Overview


The project explores an innovative approach to peptide drug delivery using the Type VI secretion system (T6SS) found in specific bacteria, addressing conventional limitations of peptide drugs by ensuring stable and precise drug delivery to target cells. Through genetic engineering and various validations, the project aspires to enhance the functionality and potential applications of peptide drugs in medical treatments. The anticipated outcome is to successfully establish a peptide drug delivery system using T6SS while acknowledging limitations related to in vivo testing due to ethical and safety considerations. The following are Biobricks we designed for the purpose of T6SS testing drug delivery of T6SS components. We’ve tested most of the components and viewed results of likely successful secretion.

Drug Delivery T6SS Components


Biobrick code Type Name Length (bp)
BBa_K4739002 Coding Hcp1 486
BBa_K4739003 Coding VgrG3 1935

Peptide Drugs


Biobrick code Type Name Length (bp)
BBa_K4739001 Coding Minihepcidin 27
BBa_K4739009 Coding Dulaglutide 831
BBa_K4739010 Coding Parkin 1398
BBa_K4739011 Coding KLA 48
BBa_K4739012 Coding Tenecteplase 1587

Fusion Protein


Biobrick code Type Name Length (bp)
BBa_K4739004 Coding Hcp-Minihepcidin fusion protein 558
BBa_K4739005 Coding VgrG3-Dulaglutide 2913
BBa_K4739006 Coding VgrG3-KLA 2130
BBa_K4739007 Coding VgrG3-Parkin 3480

Utility


Biobrick code Type Name Length (bp)
BBa_K4739000 Protein_Domain AAAGGG linker 18