Contribution

Biobricks Parts Collection

We have contributed to the iGEM Registry of Standard Biological Parts BBa_K4911000, BBa_K4911001, BBa_K4911002, BBa_K4911003.

Contribution to existing parts

We have added information to BBa_J428064, BBa_J428060, BBa_J428063. The sequence of this part was verified using sequencing. The availability of the necessary sites was checked using restriction analysis (BsaI). The part can be cloned using the RFC1000 standard.

Software

Our team has developed a model to assess the levels of expression of our system: we have created a mathematical model that allows us to determine the rate of inhibition of expression due to the work of CRISPi. We also made a graphical interface for this model.

Hardware

Our team has developed an installation design for changing the medium in a 96-well plate: we have designed a device that allows you to change the medium in a 96-well plate. For cell cultivation without additional manipulations by the experimenter.