1 Calendar
Date:
4
/
8
/2023
Event: Members training (theory)(online)
Agenda:The team leader introduced the iGEM competition content and challenge
background to the members.
Date:
4
/1
5
/2023
-5/27/2023
Event: Members training (theory) (online)
Agenda: The instructor introduced the basic research methods of modern
molecular biology to the members.
Date:6/9/2023
-
6/
1
0/2023
Event: Project
Topic
Brain storm (online)
Agenda:
We decided to focus on the diabetes issue.
Date:
6/17/2023
Event:
Project Design Brain storm（online）
Agenda:
We had a online discussion meeting with teachers
who brought us many practical inspirations.
Date:
6/24/2023
Event:
Project Design Brain storm（online）
Agenda:
We confirmed the preliminary project design plan.
Date:
7/6/2023
Event:
Project Launch Meeting（online）
Agenda:
We confirmed the final project design plan.
Date:
7
/
1
1/2023
Event: Meeting
Agenda: For the first face-to-face meeting, an ice breaking event was
held and a candidate for the leader was selected
.
All members designed the team logo, uniform, and name
together
Date: 7/
1
2/2023
Event: Members training (practical)
Agenda: The teacher conducted laboratory safety training for the
members and led the team to
drafted
a detailed
experiment plan.
Date:
7
/
13
/2023
Event: Construction of plasmids
Agenda: Construction of plastics (TRP promoter UVR8N397 BIC) by enzyme
digestion pBridge CRYN489 Nde1, Bstb1. Amplification of BIC fragments using PCR technology
Date:
7
/
14
/2023
Event: Construction of plasmids
Agenda: Agrose gel electrophoresis(BIC) and DNA gel extraction
Date:
7
/
15
/2023
Event: Construction of plasmids
Agenda: Plasmid extraction (pBridge-BD-CRY2)and Homologous recombination
(exogenous genes)(TRP1, UVR8, BIC2)
Date:
7
/
16
/2023
Event: Construction of plasmids
Agenda: Plasmid extraction (pBridge-BD-CRY2)and Homologous recombination
(exogenous genes)(TRP1, UVR8, BIC2)
Date:
7
/
17
/2023
Event: Construction of plasmids
Agenda: Homologous recombination (exogenous genes)(TRP1, UVR8, BIC2)And
the screening of resistant tablets(Amp)
Date:
7
/
18
/2023
Event: Construction of plasmids
Agenda: Plasmid extraction (pBridge-BD-CRY2)and colony PCR
Date: 7/
19
/2023
Event: Construction of plasmids
Agenda: Plasmid extraction (pBridge-BD-CRY2)and Restriction enzyme single
digestion(Nde1)
Date: 7/2
0
/2023
Event: Construction of plasmids
Agenda: Restriction enzyme digestion(Nde1 Restriction enzyme single
digestion）and pBridge-BIC2
Date: 7/
21
/2023
Event: Construction of plasmids
Agenda: Agrose gel electrophoresis and DNA gel extraction
Date: 7/
22
/2023
Event: Construction of plasmids
Agenda: Homologous recombination (exogenous genes)(TRP1, UVR8,
BIC2)
Date: 7/
23
/2023
Event: Members training (practical)
Agenda: Taking the CRY2 gene of Arabidopsis as an example, I learned how to
design gene primers using NCBI website and snapgene
Date: 7/
24
/2023
Event: Construction of plasmids
Agenda: Detection of plasmid concentration and Restriction enzyme double
digestion(Nde1 and Bstb1)
Date: 7/
25
/2023
Event: Construction of plasmids
Agenda: Agrose gel electrophoresis and DNA gel extraction
Date: 7/
26
/2023
Event: Construction of plasmids
Agenda: Shake the bacteria and prepare solid Lysogeny broth
Date: 7/
27
/2023
Event: Construction of plasmids
Agenda: Homologous recombination (exogenous genes)(TRP1, UVR8,
BIC2)
Date: 7/
28
/2023
Event: Construction of plasmids
Agenda: pBridge AD-CIB1
Date: 7/
3
1/2023
Event: Construction of plasmids
Agenda: Prep solid SD(-Trp/-Leu)
Date:
8
/
1
/2023
Event: Construction of plasmids
Agenda: Plasmid extraction (pBridge-BD-CRY2)and Restriction enzyme single
digestion(Nde1)
Date:
8
/
2
/2023
Event: Construction of plasmids
Agenda: S.cerevisiae transformation (PEG/LiAc), pBridge-AD+BD-CRY
Date:
8
/
3
/2023
Event: Construction of plasmids
Agenda: pBridge-AD-CIB+BD-CRY and colony PCR
Date:
8
/
4
/2023
Event: Construction of plasmids
Agenda: Agrose gel electrophoresis and DNA gel extraction
Date:
8
/
5
/2023
Event: Construction of plasmids
Agenda: Plasmid extraction (pBridge-BD-CRY2) and S.cerevisiae
transformation(PEG/LiAc）
Date: 8/
6
/2023
Event: Meeting
Agenda: Teacher leads team members to review recent experimental
results
Date:
8
/
7
/2023
Event: Meeting
Agenda:
Initial Wiki writing task distribution