Contribution

Experiments

  • We provide a program for SRB culture.See Methods
  • We provide a new solution for detecting biofilm maturation using crystal violet.
  • We provided a new idea of using AHLs-luxR manipulation system to characterize the change of AHLs concentration.
  • We improved the Turbidimetric bacteriostatic test protocol See Methods
See Results

Parts

  • 7 basic parts, 6 composite parts and 2 improved parts were uploaded.
  • We performed gene sequence editing on 2 basic parts and 1 improved part and verified their efficiency.
  • we completely designed and detected Quorum sensing induced suicide system (low AHLs) and provided a new suicide logic circuit

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Modeling

  • Whole process model framework in the project, including gene block selction, targeted AMP design, enzyme optimization, project feasibility validation and project application decision, which closely related to the substantive advancement of the project, and provided a feasible modeling framework for the iGEM team.
  • COBRA-AGENT framework to provide a solution for integrating microbial metabolism and spatial distribution information.
  • We design the fusion protein design of antimicrobial peptide carrier protein targeting SRB-microenvironment, which provides new ideas for AMP design.
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Human Practices

  • We investigated and reported the local status of sewer SRB and hydrogen sulfide through management and treatment.
  • We created a poster and an online video to remind residents of the importance of sewer maintenance.
  • Exhibited at the Synbio & SDG Exhibition held by team SCUT-China and SCAU-China and designed part of the public display panels.
  • We contributed our project analysis to the Bioethics Report by team McGill.
  • Education: Four educational events were held in local schools and at Sichuan University to encourage students of all ages to learn about and experience synthetic biology.
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