Results:

Unfortunately, due to difficulties in cloning, we did not achieve the results we had hoped for. For the construction of the allelic exchange vector, the fragments had to be PCR amplified. The PCR products were checked in a 1% agarose gel. It can be seen that the amplification of the gene fragments was successful. However, the fragments could not be assembled.

Figure 1: Agarose gel of the amplified PCR products of the pDM4 empty vector, upstream and downstream chromosomal part and sfGFP.