a paw-sitive addition
We designed 5 parts that future iGEM teams can utilize. Beginning with our first and second parts that we designed, which are BBa_K4734001 and BBa_K4734002, are the forward and reverse primers need to linearize the M13KE Phage Vector. We needed to place the primer at the exact spot, so that it can linearize the vector in order for us to insert our chosen epitope into the sequence. The primer is placed in the middle of the M13KE phage and the reverse and forward primers are flipped, so that it can linearize the prime in accordance with each other and the 5' and 3'. We followed a PCR amplification protocol in order to linearize the vector. Our 3rd, 4th, and 5th, parts, which are BBa_K4734003, BBa_K4734004, and BBa_K4734005, are the epitopes that we chose to utilize in our wet-lab. These epitopes include overhangs that we included when designing these parts. These parts can be directly added to the M13KE phage and will attach the chosen epitope to the phage. All 5 of these parts are essential for any choosing to replicate our protocol or participate in a phage display experiment. These primers are crucial in order insert an epitope onto the phage.
| Name | Type | Description | Designers | Length |
|---|---|---|---|---|
| BBa_K4734001 | Basic | Forward Primer for Linearizing M13KE Vector | Srika Popuri, Riya Athalye | 23 bp |
| BBa_K4734002 | Basic | Reverse Primer for Linearizing M13KE Vector | Srika Popuri, Riya Athalye | 30 bp |
| BBa_K4734003 | Basic | TNNEDEQ Epitope | Srika Popuri, Riya Athalye | 61 bp |
| BBa_K4734004 | Basic | QGNDEHSSQ Epitope | Srika Popuri, Riya Athalye | 67 bp |
| BBa_K4734005 | Basic | RBHS iGEM Designed Epitope | Srika Popuri, Ethan Kim | 109 bp |
Creating a project requires an abundance of research, and we understand it is time-consuming. Our team has created a lit review book that contains a detailed summary on the articles, research papers, and other sources that helped us throughout our project focused on Toxoplasma Gondii, so that future iGEM teams and researchers who are interested in working with Toxoplasma Gondii can have a reference to start from.We wrote shortened summaries and key points from 20 papers, which we deem as enriching to iGEM teams who are looking for the most comprehensive and relevant information out of the boundless expanse of T.Gondii related literature.
We collaborated with CCA, DNHS, and WVHS iGEM team to create a videos on Youtube about synthetic biology and our projects for those who are hoping to get into the lab and do synthetic biology work. Our videos covered our project and our implementation for this year and basic synthetic biology practices. Although the information we covered was pretty basic, they are crucial to know for anyone working in synthetic biology, and we hope that this video benefits any beginning iGEM teams who are starting out in the lab.