We appreciate all the companies, doctors, professors, teachers, other iGEM teams, every participant in our education, communication, and survey activities, and everyone who interacted with our project. They all provided us useful advice and inspired us for advanced ideas.
For more information, please visit our Attribution page.
In our project, we design to make a portable, fast detection device to detect the inflammation symptoms. We intended to use customers’ saliva for the detection. When the biomarker detects the target and binds to the molecule, it suppresses the signal of the following gene expression. Thus, it will reduce the intensity of the fluorescent intensity of mCerulean and suppress the extracellular electron transfer system. Then the device will collect the data from the E.coli, and transform it into the electronic data.
Another part in our project is to construct a probiotic E.coli that can improve the hesperetin synthesize in our body. We designed a strain of E.coli Nissle 1917 to secrete rhamnosidase, which can transform hesperidin to hesperetin.
For more information, please visit our Description page.
Our project, Vigila Guard, is a valuable approach that provides the detection of inflammation symptoms on-time. Our bio-device can get the data of the inflammation degree in the human body, which can be contributed to the medical and diagnose system.
Our engineered E.coli strain Nissle 1917 (EcN) is a probiotic, which can secrete rhamnosidase to make hesperetin from hesperidin, which is contributed to the medical system too.
For more information, please visit our Contribution page.
The engineering cycle involved design, build, test, and learn. In our project, we have followed the whole engineering cycle all the time. For the plasmid construction, firstly, we designed three different ways of downstream gene expression, FP, EET, and α-Rhamnosidase. Then, we conducted the experiment to build up these modified plasmids. To test whether the plasmids have been constructed successfully or not, we observed the bacteria growth status on the plate with a selection marker. From several times of trials and errors, we learned the failure might have resulted from the process of ligation and the poor concentration of DNA fragments. As for the device, we designed a portable, timely device initially. We used the techniques of 3D printing and Arduino board to build on our device. But, after testing our device, we found some problems. So, we learned the shortcomings of our devices and made some adjustments to improve our devices.
For more information, please visit our Engineering page.
This year, the NYCU-Taipei iGEM team has observed the rising demand of preventive healthcare. As a result, we set up a goal to build up a bi-functional, on-site and timely bio-device. It incorporates the functions of sensing and treatment. During our human practice process, we got close to our potential users who lack sufficient medical care since we have communicated with them to make our project satisfy their demands. Also, we consulted expert’s advice to make our project more realizable and improve our project once and once again. Last but not least, we not only collaborated with other iGEM teams but also held education activities in our school to make more people recognize the issue we pay attention to.
For more information, please visit our Human Practices page.
We selected plenty of gene from variant spricies, including Streptomyces avermitilis, Staphylococcus aureus. We achieve construction of saeRS and phla from Staphylococcus aureus and achieved to make these parts a workable Zn2+ detector.
For more information, please visit our Parts page.
Our collection including biomarker sensing and extracellular electron transfer pathway. The biosensing collection has already been proofed to response Zn2+ concentration with notable corelation between 1.0~1.4mM Zn2+.
For more information, please visit our Parts page.
Given that the existing conventional techniques for measuring fluorescent protein and extracellular electron transfer rely on huge and costly equipment, we have undertaken the development of an inexpensive and compact device, aiming to offer an efficient and readily available solution for such measurements. Our objective is to provide future teams with a valuable tool for accurately assessing fluorescent protein and extracellular electron transfer signals. All the details can be found on our Hardware page.
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The repository used to create this website is available at gitlab.igem.org/2023/nycu-taipei.