As a contribution, we have designed a saliva health testing device to make contributions in various aspects.
Our device aims to enable rapid detection of the levels of Calprotectin and L-tyrosine in saliva, both of which are biomarkers associated with body inflammation. The Saep3+(Mut-SaeRS+tag)+BBa_B0015 construct that we have implemented can effectively capture Zn2+ and influence the downstream expression of fluorescent proteins. This, in turn, allows us to gauge the levels of both biomarkers in saliva (for more information, refer to the experiments page). By interpreting the results on our device, users can ascertain the biomarker levels in their bodies and promptly decide whether to seek further medical attention. We believe that our device not only enhances the convenience of at-home disease screening but also helps bridge the gap in medical resources between urban and rural areas.
Whether applied in Taiwan or on a global scale, these contributions have the potential to significantly enhance human well-being.
We hope to provide people from all over the world to use our tool according to their needs. Conventionally, we could only use very expensive and large machines to measure fluorescence and electric current. To provide a better option for measurement, we decided to construct two separate devices to accommodate our fluorescent protein and electron transfer measurement. We used 3D printing to design and print out our device, 3D printing provided us with a simple assembly method, allowing us to compartmentalize and separate bacteria and electronic circuits.
With these two devices, the measurement of fluorescence and electric current becomes affordable, portable, and easy to assemble. The device design is accessible to everyone, enabling them to use our tool for their own needs. Everything needed to build our device can be found on our Hardware page, including 3D printing design and materials.
We also provided the other teams with affordable and easy-to-assemble devices and tutorials for building our device for easier measuring of fluorescence and extracellular electrons. For more information, please visit the Hardware page.
To expand the use of our device to future teams or other research applications, we chose the LED as our light source, which can control the RGB value of the emission light. This character of our device can be EXTREMELY IMPORTANT. The kind of fluorescent protein (FP) we detect by our device won’t be limited to mCerulean, but can detect any kind of FP since we can give the corresponding excitation light of that specific FP. The controlling code for the light source can be found on the Hardware page.
To enhance user experience, we designed two versions of the app, one can be installed on the computers, and one can be installed on the mobile phone. Our apps provide the function for users to create a personal account, and in our backstage, we can record the testing results of individuals. Users using the app on mobile phones can upload the testing results to their Google Drive. For more information about the programming and design of our app, please visit the Software page.
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