July
2th
WET&DRY TEAM:
After a quick break, Dr. Xie, the instructor of the wet team, assisted to interpreted the whole project design and we determined the daily schedule. In the afternoon, we had a brainstorm and decided the team name, Logo, VI, Mission, Slogan, team, team uniform, merch and created official account on social media. Then we held the election for the preliminary leaders for team management.
3th
WET TEAM:
l. Configuring LB Solid Medium
li. PCR preparation
lii. Culturing Escherichia Coli
liii. preparing LB culture medium
DRY TEAM:
In the morning, we did some training for the IHP (include questionnaire and interview), we confirmed the general direction of the implementation and the questionnaire. After the lunch break, the team member finish the writing of the implementation and the outline of the interview. We also got familiar with the first education part we were going to start tomorrow.
4th
WET TEAM:
liv. Centrifuging E.Coli in LB Broth
lv. Plasmid Enzyme cutting/digestion
lvi. Gel electrophoresis
lvii. Purification of target DNA
a. Purification of the target gene involves isolating the specific DNA sequence of interest from a complex mixture.
lviii. Gel recycle
DRY TEAM:
In the morning, we decided the format and person in charge of the public tweets on Wechat social account and three online education activity. We completed the first draft of questionnaire 2. In the afternoon, we conducted an interview with Dr. Qin Ruomeng (including material arrangement), completed the first tweet, posted the first questionnaire to the public, and wrote the outline of the second interview.
5th
DRY TEAM
In the morning, we finalized the visit plan for the afternoon of July 7 at Lujiazui Street, finished writing the daily tweets for the public website, and finalized the outline of Interview 2 and the link to the meeting; in the afternoon, we finished recording the video of Online Edu1, wrote the outline of Interview 3, and completed the PPT and speech of the popularization of science for the elderly; because Professor Shen suddenly had a very difficult task to handle, Interview 2, which was scheduled for tonight, was rescheduled to tomorrow night.
WET TEAM：
l. continuing gel electrophoresis and recycling
li. T4 DNA ligase connects vectors and fragments
a. To perform heat shock transformation of E. coli and facilitate the docking of the target DNA with the vector
lii. PCR product purification
a. To select the target DNA using a centrifuge and purify it using a wash solution
6th
DRY TEAM:
During the day the team took business planning classes, and at night they conducted a second interview with Professor Shen and prepared for tomorrow's interview materials.
WET TEAM:
l. Colony PCR
a. Using pcr thermocycler to verify our colony
li. Plasmid Extraction
a. Using plasmid to seperate solution and the cell debris
Gel electrophoresis
For checking and finding the correct strand of target DNA
7th
DRY TEAM:
In the morning we made the final preparation and completed our business plan. We went to a senior center in the afternoon and interviewed the staff members.
WET TEAM:
l. Plasmid extraction
a. The plasmid extraction method is to remove RNA, separate the plasmid from the bacterial genome DNA, and remove proteins and other impurities to obtain relatively pure plasmids.
li. Culture of HEK293 cells
a. To culture HEK293 cells in an appropriate broth at a temperature of 37°C and at a concentration of 5% CO₂ to obtain the raw material for cell transfection.
lii. Cell transfection
a. Change the DMEM culture medium for HEK293 cell for nutrients.
b. Mix DMEM, α2A-AR-Rluc8 plasmid, Venus K-Ras plasmid, and PEI into 6 centrifuge tubes for cells to be fed to promote growth.
c. Put into an incubator for cells to conseve energy and prolong their viability and cell recovery.
liii. LB culture media configuration
liv. Gel electrophoresis
8th
DRY TEAM
We conducted the EDU Workshop the entire day and completed the Edu block activity design. We also completed the interview records at the senior center yesterday and published the third article.
WET TEAM:
l. WIKI Documentation
li. RUSH-BRET and protein purification lecture
lii. enzyme digestion, connection
liii. Agarose gel purification
liv. adding IPTG
lv. BRET expirament
lvi. cell transfer
9th
DRY TEAM:
In the morning we completed the preparations for our pitch. In the afternoon we conducted our pitch and completed our interview with the business expert. Completed the promotion video’s design, and discussed our business plan book
WET TEAM:
l. making SDS-PAGE gel
li. cell transferring
lii. Protein purification and gel produce
10th
DRY TEAM: Day off
WET TEAM:
l. BRET excitement - 1
li. Taking Gel purification and extraction picture
lii. Continue writing wiki
11th
DRY TEAM:
Finished up the promotion video recording. Finished the PowerPoint slides show creation. We went to Tianlin Road and take class for the children, introduce Alzheimer’s disease to them.
WET TEAM: Day off.
12th
WET&DRY TEAM:
Today we take group photos in the morning and after the lunch break we learn how to do a presentation. We learn how to abstract key point. I'm in charge of the project goal part of the tomorrow's whole team's speech
13th
WET&DRY TEAM:
Internal presentation session and review meeting for the next step of the project.
Midterm Break
August
14th
WET TEAM:
l. Culture of HEK293 cells
li. Cell transfection
15th
WET TEAM:
l. Add IPTG
li. Prepare BRET samples
liii. making SDS-PAGE gel
16th
WET TEAM:
l. Protein purification and concentration
li. Sent protein to Nanjing for IP-MS
lii. Sent cell samples to SubCat for BRET
17th
WET TEAM:
l. Received BRET data for analysis
24th
WET TEAM:
li. Received IP-MS result and analysis for wiki documentation
