Wear gloves and lab coats
Wear shoes with close ends
No eating, drinking or running in Lab
Tie up long hair
Dispose biological wastes in specific bins
Carry our experiments with poisonous or pungent gases in a fume cupboard
Clean up the lab before leaving
Operation of the clean bench/biosafety cabinet
Sterilize the cabinet by opening UV light before usage for 30 min
Do not stare directly at the UV light
Open laminar airflow before usage
Sterilize all apparatus over the alcohol burner before using them
All caps and lids should be placed with their bottoms up, and sterilized above the flame before usage
Spray alcohol and scrub hands
Operation using a pipette
The non-disposable part of the pipette shouldn’t be in contact with any reagents to prevent contamination
Operations using Alcian blue dye
Carry out all procedures in a fume cupboard
Operations regarding gel electrophoresis
Wear gloves as the gel is poisonous
The bacteria our team only uses in the experiment is E. coli DH5α, a non-pathogenic strain of microorganisms. This is the most commonly used strain in genetic engineering. We cultured bacteria of E. coli DH5α strain in a laminar flow clean bench throughout the whole process of gene cloning. Additionally, in consideration of the environmental and ecological issues, all substances contaminated by E. coli DH5α will be sterilized before disposal and will be discarded in the biohazardous bin. In addition, we also used the ATDC5 cell line to construct the skeletal cell model for evaluation experiments and the relevant experiments were conducted inside the biosafety cabinet and the cell room under the supervision of our instructors.
All enzymes and genetic materials (including pCMV-3Tag-1A, RNAi-Ready pSIREN-RetroQ, EcoRI, XhoI, BamHI, T4 ligase and so on) present minimal hazards to the environment and human health. All lab apparatus was sterilized or cleaned before use.
Our experiments don’t involve any human or animal. And all genetic modifications that were established in our process are limited to research purposes.