2023/08/03：

ATDC5 Cell recovery and differentiation by the instructor Ms. Huang.

2023/08/09：

Ice breaking activities, team cooperation games, design of team name,slogan and logo, choose team uniforms.

2023/08/10：

Visited the lab, prepared LB solid medium, high temperature autoclave sterilization, prepared LB solid medium plate, cell passage, transformed and cultivated E. coli strain.

2023/08/11：

Selected and cultivated monoclonal colonies, review the experimental outline.

2023/08/12：

Extracted DNA of plasmid from bacteria solution, agarose gel electrophoresis, DNA gel extraction, cell passage.

2023/08/13：

ATDC5 cells transformation, Alcian blue dyeing, prepared Tris-HCl solution with different pH values (for configuring SDS-PAGE electrophoresis gel).

2023/08/14：

Add IL-1β (10 ng/mL) to the growth medium of ATDC5 cells, capture pictures of ATDC5 cells stained with Alcian blue dye, prepared transfer buffer for western blot, prepared SDS-PAGE electrophoresis gel, transformed and cultivated E. coli strain.

2023/08/16：

Western blot, qRT-PCR, extracted DNA of plasmid from bacteria solution, agarose gel electrophoresis, DNA gel extraction.

2023/08/17：

Western blot, qRT-PCR.

2023/08/19：

Result Analysis: Western blot and qRT-PCR.

2023/08/20：

Team meeting.

2023/08/20：

Team meeting.

2023/08/21：

Cells were transfected with sh-1, sh-2, and sh-N plasmid, and the cells were transfected with fluid.

2023/08/22：

Add IL-1β to the growth medium of ATDC5 cells for stimulation.

2023/08/23：

Cell protein and total RNA were extracted.

The expression level of OGN protein was investigated by Western blot.

2023/08/24：

qRT-PCR: investigation of the transcriptional levels of OGN and other matrix metabolism-related genes, including Col2, Aggrecan, Sox9, MMP3 and MMP13.

2023/08/25

Result Analysis: qRT-PCR.