Hardware
PVA Hydrogel
We are the first iGEM project that successfully use PVA Hydrogel for B. subtilis whole -cell biosensor construction for higher effectiveness of hydrophobic toxin detection
Potential Implementation
Our hardware design, PVA Hydrogel, can help organisms grow according to experimental parameters and execute their engineered function.
Future iGEM teams could construct PVA Hydrogel as a new option for whole cell biosensors, especially if they are testing hydrophobic toxins.
Dry Lab
Modelling
Proposed method of using 2-step fit: GA and lsqcurvefit in MatLab for both global parameters space searching and local optimization.
Cereulide production model: give
Visualisation code for turning 2D to 3D matrix
Image thresholding and cropping
Simplified version of rotation matrix function
Visualisation using tinker
Circuit
The promoter PsinI reporter is tested by exposing the transformed B. subtilis to different concentrations of cereulide and its structurally similar ionophore, valinomycin
Characterization of PsinI-RFP reporter system (BBa_K4725012)
The behavior of the PsinI-RFP reporter system upon the addition of valinomycin and cereulide is characterized.
[https://2023.igem.wiki/hkust/experiments_circuit] [https://2023.igem.wiki/hkust/engineering]Characterization of PsinI-SpoVG-lacI-SpoVG-(Ec)SspB-SpoVG-RFP-Double Terminator - Phyperspank-SpoVG-His6(GSlinker)-GFP-(Ec)ssrA-(ALGG)+4-Double Terminator biosensor system (BBa_K4725015)
The behavior of the cereulide biosensor system upon the addition of valinomycin is characterized.
[https://2023.igem.wiki/hkust/experiments_circuit] [https://2023.igem.wiki/hkust/engineering]Seventh International InterLaboratory Measurement Study
All interlab 2023 protocol is performed to contribute to 2023 Interlab.